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Replicative senescence in human fibroblasts

ABSTRACT: Transcriptional abundance in each of senescent populations from three cell lines (WS1, WI38 and BJ) is compared with abundance in isogenic early passage proliferating cells and also with abundance in early passage quiescent cells. A strain or line experiment design type assays differences between multiple strains, cultivars, serovars, isolates, lines from organisms of a single species. Keywords: strain_or_line_design

ORGANISM(S): Homo sapiens

PROVIDER: GSE3730 | GEO | 2005/12/03

SECONDARY ACCESSION(S): PRJNA104845

REPOSITORIES: GEO

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Replicative senescence in human fibroblasts

Project description:Transcriptional abundance in each of senescent populations from three cell lines (WS1, WI38 and BJ) is compared with abundance in isogenic early passage proliferating cells and also with abundance in early passage quiescent cells. A strain or line experiment design type assays differences between multiple strains, cultivars, serovars, isolates, lines from organisms of a single species. Computed

2005-12-02 | E-GEOD-3730 | biostudies-arrayexpress

Transcription profiling of human fibroblasts, senescent populations from three cell lines (WS1, WI38 and BJ) is compared with abundance in isogenic early passage proliferating cells and also with abundance in early passage quiescent cells

2005-10-07 | E-SMDB-1951 | biostudies-arrayexpress

Replicative senescence in post-selection HMECs

Project description:Transcriptional abundance in each of senescent populations from two post-selection HMEC lines (48R and 184) is compared with abundance in isogenic early passage proliferating cells and also with abundance in early passage quiescent cells. A strain or line experiment design type assays differences between multiple strains, cultivars, serovars, isolates, lines from organisms of a single species. Keywords: strain_or_line_design

2005-12-03 | GSE3731 | GEO

Replicative senescence in post-selection HMECs

2005-12-02 | E-GEOD-3731 | biostudies-arrayexpress

Transcription profiling of replicative senescence in post-selection HMECs, senescent populations from two post-selection HMEC lines (48R and 184) is compared with abundance in isogenic early passage proliferating cells and also with abundance in early passage quiescent cells

2005-10-07 | E-SMDB-1952 | biostudies-arrayexpress

miRNA Expression in Senescent and Immortalized BJ fibroblasts

Project description:Limited data exists regarding changes of microRNA (miRNA) expression during senescence in human cells and no reports correlate telomerase expression with regulation of senescence-related miRNAs. We used miRNA microarrays to provide a detailed account of miRNA profiles for early passage and senescent human foreskin (BJ) fibroblasts as well as early and late passage immortalized fibroblasts (BJ-hTERT) that stably express the human telomerase reverse transcriptase subunit hTERT. The revelation that miRNA expression changes with extended passaging in BJ-hTERT cells will contribute to a comprehensive understanding of the connections between telomerase expression, senescence and processes of cellular aging.

2010-06-05 | GSE22134 | GEO

p53-repressed miRNAs are Involved with E2F in a Feed Forward Loop Promoting Proliferation

Project description:miRNA expression profiles of WI38 primary human fibroblasts with an active or inactive p53. Cells were compared under normal untreated conditions (young and proliferating cells), after DNA damage with Doxorubicin, and upon entry into replicative senescence. Keywords: miRNA, WI-38, p53, GSE56, Senescence, Doxorubicin, Cancer, DNA-damage, fibroblasts. 6 samples of WI38 cells were analyzed on 12 Exiqon miRcurry LNA arrays in biological duplicates (2 different cell culture plates for each experimental condition). The six conditions included: 1. [Con_Young] - Primary Young WI38 cells (passage 20) with a control retroviral vector (pLXSN-NEO). Untreated. 2. [GSE_Young] -Primary Young WI38 cells (passage 20) with a retroviral vector encoding for the p53-inactivating peptide GSE56 (pLXSN-NEO-GSE56).Untreated. 3. [Con_Dox] -Primary Young WI38 cells (passage 20) with a control retroviral vector (pLXSN-NEO). Treated with Doxorubicin (0.2 micrograms/ml) for 24 hours). 4. [GSE_Dox] Primary Young WI38 cells (passage 20) with a retroviral vector encoding for the p53-inactivating peptide GSE56 (pLXSN-NEO-GSE56). Treated with Doxorubicin (0.2 micrograms/ml) for 24 hours). 5. [Con_Old] - Sesescent WI38 cells (passage 30) with a control retroviral vector (pLXSN-NEO). Untreated. 6. [GSE_Old] - Senescent WI38 cells (passage 26) with a retroviral vector encoding for the p53-inactivating peptide GSE56 (pLXSN-NEO-GSE56).Untreated. RNA was extracted with TRI-Reagent and sent for labeling and hybridization in Exiqon laboratories (In Denamark). Samples were labeled with Cy5. Reference sample (Cy3) was an RNA mix of all samples. Log2 for Ratio(Cy5/Cy3) was used for further analysis.

2010-05-19 | E-GEOD-12450 | biostudies-arrayexpress

Gene expression profile of replicative senescence in normal human diploid fibroblasts.

Project description:Transcriptional profiling of normal human diploid fibroblasts(TIG-3) comparing late passage with early passage.

2014-04-10 | GSE49860 | GEO

Replicative Senescence in Primary Fibroblasts

Project description:Senescent passage 26 fibroblasts compared to proliferating passage 8 fibroblasts Keywords: Senescent vs. Proliferating

2007-01-24 | GSE6762 | GEO

The proteomic study of serially passaged human skin fibroblast cells uncovers down-regulation of SMC4 involved in replicative senescence

Project description:In this study, a label-free quantitative proteomic approach was employed to analyze the serial passaged human skin fibroblast （CCD-1079Sk） cells, within 3305 proteins quantified. Of which, 372 proteins were significantly changed in early passage(P6), middle passage (P12) and later passage (P21), with a time-dependent decrease or increase tendency. Then, of which, SMC4 was selected for further biological validation. The results confirmed that the expression of SMC4 was significantly down-regulated in a time-dependent manner in the subculture of human skin fibroblasts (HSFb) with Western Blot experiment.

2018-10-22 | PXD007732 | Pride

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