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Expression data of CD44loCD8+ T cells from sanroque versus wild-type mice

ABSTRACT: We used microarrays to detect the primary changes caused by the 'san' mutation in Roquin gene by comparing the gene expression profiles of naive (CD44lo) CD8+ T cell population.

ORGANISM(S): Mus musculus

PROVIDER: GSE37319 | GEO | 2012/04/17

SECONDARY ACCESSION(S): PRJNA159571

REPOSITORIES: GEO

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Expression data from CD44loCD8+ T cells of sanroque versus wild-type mice

Project description:We used microarrays to detect the primary changes caused by the 'san' mutation in Roquin gene by comparing the gene expression profiles of naive (CD44lo) CD8+ T cell population. Sorted CD44loCD8+ T cells of age-matched sanroque (Roquinsan/san) and wild-type (Roquin+/+) mice in C57BL/6 background were subjected to RNA extraction and hybridization on Affymetrix microarrays.

2012-04-16 | E-GEOD-37319 | biostudies-arrayexpress

MiRNAs sequence of macrophages derived exosoms in WT group and Roquin-1 san:san group.

Project description:We reported the function of Roquin-1 in the miRNA-sorting of macrophages derived exosomes. At first, we used the supernatant of 929 cells to culture the bone marrow derived macrophages (BMDM) from bone marrow cells of WT and Roquin-1 san:san mice. Then, we isolated the macrophages derived exosomes by ultracentrifugation. At last, we performed Next-generation sequencing to detect the differences of miRNA-sorting between WT and Roquin-1 macrophages derived exosomes.

2021-03-20 | GSE169215 | GEO

Project description:Define,(a) intrinsic differences and (b) changes occuring upon TCR activation in genetic profiles of naive CD4+ and CD8+ T cells from young and old animals, to identify candidate genes altered in old T cells involved in impairement of immune response in order to restore immune function in the old. Activation dependant time series on sorted naive (CD62Lhi/CD44lo) CD4 and CD8 T cells from young and old animals-Each time point contains RNA pooled from 4 independent sortings

2011-03-24 | E-GEOD-28165 | biostudies-arrayexpress

Longitudinal expression data from CD8+ T cells responding to LCMV-Armstrong or LCMV-Clone 13

Project description:During acute viral infections, naïve CD8+ T cells differentiate into effector CD8+ T cells and, after viral control, into memory CD8+ T cells. Memory CD8+ T cells are highly functional, proliferate rapidly upon reinfection and persist long-term without antigen. In contrast, during chronic infections, CD8+ T cells become “exhausted” and have poor effector function, express multiple inhibitory receptors, possess low proliferative capacity, and cannot persist without antigen. To compare the development of functional memory T cells with poorly functional exhausted T cells, we generated longitudinal transcriptional profiles for each. Naive CD44Lo CD8+ T cells were isolated and sorted from uninfected C57BL/6 mice and H2-Db GP33-specific CD8+ T cells were sorted using MHC-I tetramers at d6, 8, 15, and 30 p.i. with either LCMV Arm or LCMV clone 13. RNA from these CD8+ T cells was processed, amplified, labeled, and hybridized to Affymetrix GeneChip MoGene 1.0 st microarrays

2012-11-23 | E-GEOD-41867 | biostudies-arrayexpress

Comparison of mouse naïve (CD44lo) CD8+ T cells sorted into the highest and lowest 20% with respect to CD5

Project description:Lymphocytes from spleen and lymph nodes of unimmunized adult C57BL/6 mice were isolated, stained with antibodies for flow cytometry, and sorted into the CD8+ CD44lo CD5hi and CD5lo pool Lymphocytes from spleen and lymph nodes of unimmunized adult C57BL/6 mice were isolated, stained with antibodies for flow cytometry, and sorted into the CD8+ CD44lo CD5hi and CD5lo pool

2014-10-08 | E-GEOD-62142 | biostudies-arrayexpress

Comparison of mouse naïve (CD44lo) CD8+ T cells sorted into the highest and lowest 20% with respect to CD5

2014-10-08 | GSE62142 | GEO

Genome-wide fitness gene identification reveals Roquin as a potent suppressor of CD8 T cell expansion and anti-tumor immunity

Project description:Robust expansion of adoptively transferred T cells is a prerequisite for effective cancer immunotherapy, but how many genes in the genome modulate T cell expansion remains unknown. Here, we performed in vivo and in vitro CRISPR screens to systematically identify genes influencing CD8 T cell expansion. In the mouse genome, ~ 2,600 and ~ 1,500 genes were required for optimal CD8 T cell expansion in vivo and in vitro, respectively. In vivo-specific CD8 T cell essential genes were enriched in metabolic pathways including mitochondrial metabolism. The strongest repressor of CD8 T cell expansion was Roquin, ablation of which drastically boosted T cell proliferation by enhancing cell cycle progression and upregulation of IRF4. Roquin-deficiency or IRF4 overexpression potently enhanced anti-tumor immunity. These data provide a functional catalog of CD8 T cell fitness genes, and suggest targeting Roquin-IRF4 axis is an effective strategy to enhance efficacy of adoptive transfer therapy for cancer.

2021-10-27 | GSE186284 | GEO

Analysis of CD8+CD45RClow Tregs from CD40Ig allograft recipients versus naive

Project description:This experiment compare the transcriptional profile of CD8+CD45RClow Tregs from naive untreated rats compared to CD40Ig-treated tolerant allograft recipients.

2015-11-30 | E-MTAB-3535 | biostudies-arrayexpress

Gene profiling of naive and Listeria monocytogenes-induced memory CD8 T lymphocytes in homeostatic condition and after stimulation.

Project description:Transcriptome analysis comparing naive and Listeria monocytogenes-induced spleen memory CD8 T lymphocytes were conducted to identify key functions associated with memory CD8-mediated immune protection. Gene expression analysis was performed on quiescent and re-stimulated CD8 T cells.

2016-09-09 | GSE86601 | GEO

Gene expression analysis of Ox40-cre/ROSA-YFP CD4+ T cell subsets

Project description:An Ox40-cre allele was used for lineage marking of CD4 T cells. Naive T cells that had previously expressed OX40 demonstrated a partially activated phenotype that was distinct from that of the majority of naive T cells. These results are consistent with a minor subpopulation of naive T cells being dependent on strong signaling responses to thymic self ligands. Keywords: T cell population comparison. Characterization of a novel subpopulation of naive T cells. Four types of T cells were sorted from mice (naive, CD44lo, CD44hi and Treg).

2010-04-03 | E-GEOD-13502 | biostudies-arrayexpress

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