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Stable and inducible expression of Fbxo25 in Flp-In-293 cells: control vs. tetracycline treatment

ABSTRACT: Expression profile of human Flp-In-293-WT-FBXO25 or Flp-In-293-∆F-FBXO25 cells comparing non treated cells vs. tetraciclyne treated for 24 and 48 hours. Flp-In-293-∆F-FBXO25 cells when induced express the non functional protein (without F-box domain). The objective of the study was to identify genes up or down-regulated when Fbxo25 wild-type or Fbxo25 lacking F-box domain is superexpressed.

ORGANISM(S): Homo sapiens

PROVIDER: GSE37380 | GEO | 2012/05/15

SECONDARY ACCESSION(S): PRJNA159905

REPOSITORIES: GEO

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Stable and inducible expression of Fbxo25 in Flp-In-293 cells: control vs. tetracycline treatment

Project description:Expression profile of human Flp-In-293-WT-FBXO25 or Flp-In-293-M-bM-^HM-^FF-FBXO25 cells comparing non treated cells vs. tetraciclyne treated for 24 and 48 hours. Flp-In-293-M-bM-^HM-^FF-FBXO25 cells when induced express the non functional protein (without F-box domain). The objective of the study was to identify genes up or down-regulated when Fbxo25 wild-type or Fbxo25 lacking F-box domain is superexpressed. Flp-In-293 WT and M-bM-^HM-^FF cells with and without tetracycline treatment. Two timepoints, two replicates each.

2012-05-14 | E-GEOD-37380 | biostudies-arrayexpress

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2022-05-06 | E-MTAB-10770 | biostudies-arrayexpress

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Effects of inducible HIV-1 Vpr protein on cellular gene expression

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2006-02-10 | GSE2296 | GEO

microRNA (miRNA) expression from human 293 cells

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2010-05-22 | E-GEOD-16442 | biostudies-arrayexpress

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RNA-Seq of WT Flp-In T-REx 293, UPF3B knockout and UPF3A-UPF3B double knockout cell lines with additional siRNA-mediated control or UPF3B knockdowns

Project description:UPF3A and UPF3B are paralogous genes in human cells that are involved in the nonsense-mediated decay (NMD) pathway. NMD is a cellular quality control mechanism that monitors mRNAs during translation. Aberrant translation due to features such as the presence of a premature stop codon downstream on an exon-exon junction activates NMD and leads to the degradation of the mRNA. To investigate the role of UPF3B and UPF3A in NMD, we have generated UPF3B knockout (KO) and UPF3A-UPF3B double KO (dKO) human Flp-In T-REx 293 cells using CRISPR-Cas9. We generated RNA-Sequencing data for wildtype, UPF3B KO and UPF3A-UPF3B dKO cells with additional siRNA-mediated knockdown of Luciferase (Luc) as control or UPF3B.

2022-04-25 | E-MTAB-10716 | biostudies-arrayexpress

AP-MS analysis using Flp-In-293 cells

Project description:AP-MS analysis using Flp-In-293 cells used as a control

2020-04-01 | MSV000085212 | MassIVE

RNA-Seq of WT Flp-In T-REx 293, UPF3A knockout and UPF3A overexpression cell lines in part with additional siRNA-mediated control or UPF3B knockdowns

Project description:UPF3A and UPF3B are paralogous genes in human cells that are involved in the nonsense-mediated decay (NMD) pathway. NMD is a cellular quality control mechanism that monitors mRNAs during translation. Aberrant translation due to features such as the presence of a premature stop codon downstream on an exon-exon junction activates NMD and leads to the degradation of the mRNA. To investigate the role of UPF3B and UPF3A in NMD, we have generated UPF3A knockout (KO) human Flp-In T-REx 293 cells using CRISPR-Cas9, as well as FLAG-tagged UPF3A overexpressing cells using the PiggyBac transposon system. We generated RNA-Sequencing data for wildtype, UPF3A KO and UPF3A overexpressing (OE) cells, in part with additional siRNA-mediated knockdown of Luciferase (Luc) as control or UPF3B.

2022-08-31 | E-MTAB-10718 | biostudies-arrayexpress

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