Transcriptomics,Genomics

Dataset Information

38

The overall response of Paenibacillus larvae to bodily fluids of honeybee larvae (Apis mellifera).


ABSTRACT: Paenibacillus larvae, the causal agent of American Foulbrood disease (AFB), affects honeybee health worldwide. The present study investigates the transcriptional response of this Gram-positive, endospore-forming bacterium to bodily fluids from honeybee larvae. Four different conditions were evaluated with a loop design: sampling of in vitro grown P. larvae cultures one or four hours after addition of larval fluids or BHIT-broth (C1, T1, C4, T4). Overall design: total of 16 samples, 4 independent samples per group, 4 groups (C1, C4, T1 and T4), loopdesign, dye swap

INSTRUMENT(S): Agilent-036300 Custom Paenibacillus larvae Array (Ag-P.Larvae)

SUBMITTER: Wouter Van Delm  

PROVIDER: GSE37481 | GEO | 2014-12-22

SECONDARY ACCESSION(S): PRJNA161015

REPOSITORIES: GEO

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Publications

Effect of bodily fluids from honey bee (Apis mellifera) larvae on growth and genome-wide transcriptional response of the causal agent of American Foulbrood disease (Paenibacillus larvae).

De Smet Lina L   De Koker Dieter D   Hawley Alyse K AK   Foster Leonard J LJ   De Vos Paul P   de Graaf Dirk C DC  

PloS one 20140220 2


Paenibacillus larvae, the causal agent of American Foulbrood disease (AFB), affects honey bee health worldwide. The present study investigates the effect of bodily fluids from honey bee larvae on growth velocity and transcription for this Gram-positive, endospore-forming bacterium. It was observed that larval fluids accelerate the growth and lead to higher bacterial densities during stationary phase. The genome-wide transcriptional response of in vitro cultures of P. larvae to larval fluids was  ...[more]

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