Transcriptomics,Genomics

Dataset Information

41

Microarray analysis of gene expression profiles in human neuroblastoma cells exposed to Amyloid beta-Zn and Amyloid beta-Cu complexes


ABSTRACT: Abnormal metal accumulation is associated with Alzheimer’s disease (AD) and has a relevant role in affecting amyloid beta-peptide (Abeta) aggregation and neurotoxicity.In the present study, employing a microarray analysis of 35,129 genes, we analyzed gene expression profile changes due to exposure to Abeta-Zn or Abeta-Cu complexes in neuronal-like cells (SH-SY5Y). Microarray data indicated that Abeta-Zn or Abeta-Cu complexes selectively alter expression of genes mainly related to cell death, inflammatory responses, and apoptosis.Taken together these findings indicate that Abeta-Zn or Abeta-Cu show some commonalities in affecting AD-related target functions. The overall modulatory activity on these genes supports the idea of a possible net result leading to mechanisms that counteract toxic effects of Abeta-Zn or Abeta-Cu. Overall design: Analysis used a neuronal cell lines, the SH-SY5Y, exposed to either AB, the AB-Cu or AB-Zn complex or Cu or Zn alone. The SH-SY5Y without treatment were used that a control. For the exposure to AB, six replicates were performed (three dye swap and three non-dye swap), while for exposure to AB-Cu, AB-Zn, Zn and Cu two replicates each were made (one dye swap and one non-dye swap).

REANALYSIS of: GSE23000

INSTRUMENT(S): Human 35K OpArrays DNA microarray

SUBMITTER: Karina Fincati 

PROVIDER: GSE37661 | GEO | 2012-05-01

SECONDARY ACCESSION(S): PRJNA162645

REPOSITORIES: GEO

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Publications

Microarray analysis on human neuroblastoma cells exposed to aluminum, β(1-42)-amyloid or the β(1-42)-amyloid aluminum complex.

Gatta Valentina V   Drago Denise D   Fincati Karina K   Valenti Maria Teresa MT   Dalle Carbonare Luca L   Sensi Stefano L SL   Zatta Paolo P  

PloS one 20110127 1


BACKGROUND: A typical pathological feature of Alzheimer's disease (AD) is the appearance in the brain of senile plaques made up of β-amyloid (Aβ) and neurofibrillary tangles. AD is also associated with an abnormal accumulation of some metal ions, and we have recently shown that one of these, aluminum (Al), plays a relevant role in affecting Aβ aggregation and neurotoxicity. METHODOLOGY: In this study, employing a microarray analysis of 35,129 genes, we investigated the effects induced by the exp  ...[more]

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