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P107 knockout vs. wild type neurospheres

ABSTRACT: The ganglionic eminence was dissected and neurospheres were cultured from the brain of E13.5 embroys. RNA was isolated from neurospheres cultured from p107-/- embryos and their wildtype littermates. P107 knockout mice have previously been shown to have an increased number of stem cells and enhanced stem cell self renewal. With the microarray experiment we are hoping to discover the genes involved in stem cell number and self renewal along with p107. Keywords: other

ORGANISM(S): Mus musculus

PROVIDER: GSE3779 | GEO | 2005/12/09

SECONDARY ACCESSION(S): PRJNA93971

REPOSITORIES: GEO

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Transcription profiling by array of mouse neurospheres cultured from p107-/- embryos and their wildtype littermates

Project description:The ganglionic eminence was dissected and neurospheres were cultured from the brain of E13.5 embroys. RNA was isolated from neurospheres cultured from p107-/- embryos and their wildtype littermates. P107 knockout mice have previously been shown to have an increased number of stem cells and enhanced stem cell self renewal. With the microarray experiment we are hoping to discover the genes involved in stem cell number and self renewal along with p107. Experiment Overall Design: this experiment include 2 samples and 14 replicates

2007-12-01 | E-GEOD-3779 | biostudies-arrayexpress

Genomic mapping of E2F3 and E2F4 binding sites in neurospheres

Project description:Neural precursor cells from the ganglionic eminence at E14.5 were isolated and cultured as neurospheres. E2F3 and E2F4 genomic binding sites are mapped by ChIP-on-Chip on wild type or mutant cells.

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CUT&RUN Targeting c-FOS, c-JUN and SOX2 in neurospheres derived from mouse brain cells

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2021-04-01 | E-MTAB-9897 | biostudies-arrayexpress

Mouse neurospheres

Project description:Seven day old platelet-derived growth factor-generated neurospheres cultured from the embryonic ganglia Keywords: other

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Transcription profiling of CD133/Prominin positive neurospheres

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Real-Time Quantitative PCR Analyis of Neonatal Rat Aortic Neurospheres (ANS)

Project description:Progenitor/stem cell populations of the aorta have been poorly characterized and their self-renewal factors unknown. In this report, we demonstrate that sphere forming progenitor/stem cells, here within termed aortic neurospheres (ANS), can be cultivated from the aorta that are similar to skin-derived precursors (SKPs). Extensive mRNA transcript profiling revealed that ANS abundantly expressed the Inhibin βA transcript, of which the secreted protein dominantly formed the Inhibin βA-βA complex, or Activin A, in the proliferation medium. Co-localization of Activin A, Nestin and Sox2 was observed in the adventitial and peri-adventitial regions of the aorta, indicative of a progenitor/stem cell niche in vivo. Blockade of Activin A signaling, through the neutralization of the Type II Activin receptors ActRIIA and ActRIIB, resulted in the asymmetric differentiation, and epithelial-to-mesenchymal transition of the ANS, respectively. When treated with the latter neutralizing antibody, translocation of the ActRIIB receptor to the nucleus was observed in cells on the periphery of the sphere. Examination of the ActRIIB protein sequence showed the presence of a putative nuclear localization signal of the PY family that was conserved across species. The results from this study demonstrate that Activin A is a self-renewal factor required for maintaining ANS progenitor/stem cell identity and multipotency.

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Activin A is Required for the Self-Renewal of Aortic Sox2+ Neurospheres

Project description:Activin A is Required for the Self-Renewal of Aortic Sox2+ Neurospheres

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Transcriptome analysis of Chx10-Cre Rb1/p107 and Chx10-Cre Rb1/p107/Hells tumors

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