Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression profile in peritoneal macrophage stimulated with B-DNA or CpG-B

ABSTRACT: Toll-like receptor and RIG-I-like receptor classs may evoke or instruct a distinct type of response that is more reflective of the pathogen encountered. Although this issue may be critical to a better understanding of the regulation of immune responses to microbial infections, it has not been addressed through a direct, side-by-side comparison of the two receptor classes. To address this, we performed microarray analysis of mRNAs from peritoneal macrophages stimulated with a synthetic B-form DNA, poly(dA-dT)・poly(dT-dA) ( B-DNA) or a CpG-B oligonucleotide, which respectively activates RLRs or TLR9, and identified B-DNA inducible genes and CpG-B inducible genes.

ORGANISM(S): Mus musculus

PROVIDER: GSE37854 | GEO | 2012/05/20

SECONDARY ACCESSION(S): PRJNA163893

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Gene expression profile in peritoneal macrophage stimulated with B-DNA or CpG-B

Project description:Toll-like receptor and RIG-I-like receptor classs may evoke or instruct a distinct type of response that is more reflective of the pathogen encountered. Although this issue may be critical to a better understanding of the regulation of immune responses to microbial infections, it has not been addressed through a direct, side-by-side comparison of the two receptor classes. To address this, we performed microarray analysis of mRNAs from peritoneal macrophages stimulated with a synthetic B-form DNA, poly(dA-dT)M-cM-^CM-;poly(dT-dA) ( B-DNA) or a CpG-B oligonucleotide, which respectively activates RLRs or TLR9, and identified B-DNA inducible genes and CpG-B inducible genes. Peritoneal macrophages from B6 mice were stimulated with B-DNA (10M-NM-<g/ml) or CpG-B(3M-NM-<M) for 4 hous, and then subjected to microarray analysis.

2012-05-19 | E-GEOD-37854 | biostudies-arrayexpress

Repli-seq profile from resting (G0) and cycling (S) phase untreated B cells

Project description:In this study, we map sites of replication initiation and breakage in primary cells at high resolution under conditions of replication stress. We show that replication initiates between transcribed genes within nucleosome-depleted structures established by long asymmetrical poly(dA:dT) tracts flanking the initiation site. Paradoxically, large (>20 bp) homopolymeric (dA/dT) tracts are also preferential sites of polar replication fork stalling and collapse. We propose that the evolutionary expansion of poly(dA:dT) tracts in eukaryotic genomes serves to promote replication initiation, but at the cost of increasing chromosome fragility.

2018-08-01 | GSE116318 | GEO

Nascent RNA profile in B, and T cells untreated and upon HU treatment

2018-08-01 | GSE116317 | GEO

EdU-seq profile in B,untreated and upon HU treatment

2018-08-01 | GSE116316 | GEO

END-seq profile in B,T, and HCT116 untreated and upon HU treatment

2018-08-01 | GSE116315 | GEO

ChIP-seq profile of RPA in B upon HU treatment

2018-08-01 | GSE116320 | GEO

OK-seq profile from cycling (S) phase untreated B cells

2018-08-01 | GSE116319 | GEO

Transcription profiling of mouse wild-type, TBK1, IKKi(Ikbke), or TBK1 IKKi doubly deficient embryonic fibroblats to elucidate how TBK1 and/or IKKi mediates B-DNA-mediated innate immune responses

Project description:B-DNA-induced gene expression profile in wild-type, TBK1, IKKi(Ikbke), or TBK1 IKKi doubly deficient embryonic fibroblats; to elucidate how TBK1 and/or IKKi mediates B-DNA-mediated innate immune responses. Experiment Overall Design: Total RNA was extracted from embryonic fibroblats transfected for 4 h with or without poly(dA-dT)-poly(dT-dA), after which cRNA was synthesized. Preparation of cRNA, hybridization and scanning of the microarray were done according to the manufacturer's instructions (Affymetrix). A microarray (MG U74A version 2; Affymetrix) was used with Microarray Suite software (version 5.0; Affymetrix) and GeneSpring software (Silicon Genetics).

2007-12-07 | E-GEOD-4171 | biostudies-arrayexpress

Real-time quantitative PCR analysis of mouse RAW264.7 macrophages

Project description:Mouse RAW264.7 macrophages were treated with LPS, IFNb, poly(rI:rC), poly(dA:dT), VSV, HSV, Sendai virus. Genes identified by Human Innate Immunity Interactome for type I Interferon (HI5) were examined for expression.

2011-09-08 | GSE31790 | GEO

Dual Roles of Poly(dA:dT) Tracts in Replication Initiation and Fork Collapse

Project description:Dual Roles of Poly(dA:dT) Tracts in Replication Initiation and Fork Collapse

| PRJNA478131 | ENA