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Overexpression of ENOD40 in Arabidopsis thaliana plants reveals a role of the gene in cell wall turnover and glucosinolate accumulation.

ABSTRACT: The transcriptional and metabolic profiles of rosettes of three independent Arabidopsis thaliana ecotype Wassiliewska lines overexpressing the soybean early noduline GmENOD40 gene were compared to those of wt, and a biomarker analysis was performed. ENOD40 overexpression resulted in 382 modulated genes, 142 of which upregulated and 165 downregulated in all the three transformed lines. Cell wall, membrane, hydrolase activity on glycosidic bonds, defense response and response to stimulus gene categories were overrepresented in the modulated genes compared to TAIR complete database. Metabolomics analysis (through liquid chromatography-mass spectrometry) allowed to putatively identify 13 different glucosinolates, some flavonoids, sinapic and ferulic acid derivatives, hydroxybenzoic acid derivatives, non aromatic organic acids, amino acids. Positive genes biomarkers of the transformed plants were mainly involved in cell wall turnover, whereas positive metabolite biomarkers were the methylthioalkyl-glucosinolates.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE37867 | GEO | 2015/05/08

SECONDARY ACCESSION(S): PRJNA164675

REPOSITORIES: GEO

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Overexpression of ENOD40 in Arabidopsis thaliana plants reveals a role of the gene in cell wall turnover and glucosinolate accumulation.

Project description:The transcriptional and metabolic profiles of rosettes of three independent Arabidopsis thaliana ecotype Wassiliewska lines overexpressing the soybean early noduline GmENOD40 gene were compared to those of wt, and a biomarker analysis was performed. ENOD40 overexpression resulted in 382 modulated genes, 142 of which upregulated and 165 downregulated in all the three transformed lines. Cell wall, membrane, hydrolase activity on glycosidic bonds, defense response and response to stimulus gene categories were overrepresented in the modulated genes compared to TAIR complete database. Metabolomics analysis (through liquid chromatography-mass spectrometry) allowed to putatively identify 13 different glucosinolates, some flavonoids, sinapic and ferulic acid derivatives, hydroxybenzoic acid derivatives, non aromatic organic acids, amino acids. Positive genes biomarkers of the transformed plants were mainly involved in cell wall turnover, whereas positive metabolite biomarkers were the methylthioalkyl-glucosinolates. The transcriptional profile of rosettes of three independent Arabidopsis thaliana ecotype Wassiliewska lines overexpressing the soybean early noduline GmENOD40 gene were compared to those of wt, and a biomarker analysis was performed.

2015-05-08 | E-GEOD-37867 | biostudies-arrayexpress

Project description:Ferulic and para-hydroxybenzoic acids have different effects on cucumber seedling rhizosphere fungal communities

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Project description:4-Hydroxybenzoic acid (4-HB) is the natural precursor for CoQ biosynthesis. Hydroxybenzoic acids derivatives (HBAs) have been recently used for the experimental treatment of primary CoQ deficiency. However, the mechanisms of these therapeutic effects remain obscure. In this study, we performed an analysis in plasma to evaluate the effect of supplementation with-resorcylic acid (-RA) or vanillic acid (VA), two HBAs potentially useful for the treatment of Coenzyme Q (CoQ) deficiencies, in an animal model of mitochondrial encephalopathy due to CoQ deficiency. The results help in the identification of potential biomarkers and/or endocrine factors that contribute to the pathophysiologic characteristics of the disease and its response to treatments, supporting important scientific and medical impacts for the use of -RA or VA in the treatment of CoQ deficiency.

2022-07-15 | PXD030303 | Pride

Partial deficiency of isoleucine impairs alters transcript levels of the genes involved in branched-chain amino acid and glucosinolate metabolism in Arabidopsis

2012-11-07 | GSE42060 | GEO

Membrane proteomics of Arabidopsis glucosinolate mutants cyp79B2/B3 and myb28/29

Project description:in our previous work we studied the molecular networks cotrolling their metabolism in Arabidopsis thaliana on the level of soluble proteome and metebolome using myb28/29 and cyp79B2/B3 as mutants for aliphilic and indollic glucosinolates production respectively.we go deeper to study the changes on the level of membrane and membrane associated proteins in both mutants.

2017-06-19 | PXD005781 | Pride

Mouse mitochondrial proteome in the kidney and the brain

Project description:Hydroxybenzoic acids derivatives (HBAs) are natural phenolic compounds that are being used for the experimental treatment of primary CoQ deficiency. Some of these compounds are also able to reduce the animals body weight. However, the mechanisms of these therapeutic effects remain obscure. In this study, we used an analysis of the mitochondrial proteome to evaluate the effect of a high dose of-resorcylic acid (-RA), an HBA potentially useful for the treatment of Coenzyme Q (CoQ) deficiencies, in wild-type animals. The results highlight novel molecular mechanisms of HBAs and provide a translational perspective for the use the -RA in the treatment of CoQ deficiencies, overweight and obesity.

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Depolymerization of plant cell wall glycans by symbiotic human gut bacteria (Bacteroides ovatus)

Project description:Symbiotic bacteria inhabiting the distal human gut have evolved under intense pressure to utilize complex carbohydrates, predominantly plant cell wall glycans abundant in our diets. These substrates are recalcitrant to depolymerization by digestive enzymes encoded in the human genome, but are efficiently targeted by some of the ~103-104 bacterial species that inhabit this niche. These species augment our comparatively narrow carbohydrate digestive capacity by unlocking otherwise unusable sugars and fermenting them into host-absorbable forms, such as short-chain fatty acids. We used phenotype profiling, whole-genome transcriptional analysis and molecular genetic approaches to investigate complex glycan utilization by two fully sequenced and closely related human gut symbionts: Bacteroides thetaiotaomicron and Bacteroides ovatus. Together these species target all of the common glycosidic linkages found in the plant cell wall, as well as host polysaccharides, but each species exhibits a unique ‘glycan niche’: in vitro B. thetaiotaomicron targets plant cell wall pectins in addition to linkages contained in host N- and O-glycans; B. ovatus uniquely targets hemicellulosic polysaccharides along with several pectins, but is deficient in host glycan utilization.

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Depolymerization of plant cell wall glycans by symbiotic human gut bacteria (Bacteroides thetaiotaomicron)

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Metabolic signatures induced by real and simulated herbivory in black mustard (Brassica nigra)

Project description:LC-TOF-MS analysis of black mustard leaves exposed to methyl-jasmonate and caterpillar herbivory by Pieris brassicae. Metabolites: glucosinolates, phenylpropanoids (sinapic acid derivatives, and flavonol glucosides).

2019-08-08 | MSV000084183 | MassIVE

Partial deficiency of isoleucine impairs alters transcript levels of the genes involved in branched-chain amino acid and glucosinolate metabolism in Arabidopsis

Project description:Isoleucine (Ile) is one of the branched-chain amino acids (BCAAs) that are essential substrates for protein synthesis in all organisms. In this study, we characterized an Arabidopsis mutant, lib (low isoleucine biosynthesis), that has defects in both cell proliferation and cell expansion processes during root development. This mutant carries a mutation in OMR1 gene that encodes a threonine deaminase/dehydratase (TD) which results in a defect in isoleucine production. Microarray analysis indicated that the partial deficiency of Ile in the lib mutant triggers a decrease in transcript levels of the genes encoding the major enzymes involved in the BCAA degradation pathway; the analysis also indicated that many genes involved in the biosynthesis of methionine-derived glucosinolates are up-regulated. Total RNAs were isolated from three replicate samples of the root tissues of 8-day-old wild type (w) and lib mutant (m)seedlings grown on 1/2 MS medium and hybridized on Affymetrix ATH1 microarrays. The changes of gene expression in the lib mutant (m) was compared with the wild type (w). The corrected P value of <0.05 was used to select the genes for comparison, and only those genes whose expression was two-fold higher or lower in lib than in the WT were used for analysis.

2012-11-07 | E-GEOD-42060 | biostudies-arrayexpress

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