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Real-time quantitative PCR analysis of Human Umbilical Vein Endothelial Cells (HUVEC)

ABSTRACT: In order to identify new markers of vascular cell senescence with potential in vivo implications, primary cultured Human Umbilical Vein Endothelial Cells (HUVECs), were analysed for microRNA (miR) expression.

ORGANISM(S): Homo sapiens

PROVIDER: GSE37972 | GEO | 2012/05/16

SECONDARY ACCESSION(S): PRJNA167024

REPOSITORIES: GEO

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Real-time quantitative PCR analysis of Human Umbilical Vein Endothelial Cells (HUVEC)

Project description:In order to identify new markers of vascular cell senescence with potential in vivo implications, primary cultured Human Umbilical Vein Endothelial Cells (HUVECs), were analysed for microRNA (miR) expression. QRT-PCR microRNA expression profiling in 3 senescent (XIII passage) vs. 3 young HUVECs (II passage).

2012-05-15 | E-GEOD-37972 | biostudies-arrayexpress

HUVEC Gene Expression of Irradiated Human Umbilical Vein Endothelial Cells

Project description:Ionizing radiation has become common for diagnostic and therapeutic purpose. Since it affects the cells at genetic level, it becomes necessary to evaluate its affects especially on endothelial cells which comprises the blood vessels and thus contributes in migration of blood cells, nutrients, oxygen etc. Human Umbilical Vein Endothelial Cells (HUVECs) are major model system used in vitro to study the nature and behaviour of endothelial cells.

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Expression data from Human Umbilical Vein Endothelial Cells

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Expression profiling in Human Umbilical Vein Endothelial Cells (HUVEC)

Project description:Whole transcriptome gene expression profiling of Normal -(HUVEC) Human Umbilical Vein Endothelial Cells The HUVEC gene expression results analyzed in this study are further described in Kustermann S. et al. (2014) A real-time impedance based screening assay for drug induced vascular leakage. A NimbleGen Homo sapiens Expression Array [100718_HG18_opt_expr] study using total RNA recovered from HUVECs grown until 80% confluency. Each microarray measures the expression level of 23,611 genes using 45,033 probe sets with three 60-mer probes (PM) per probe set. Each probe set is represented once on the array.

2014-01-07 | E-GEOD-52044 | biostudies-arrayexpress

MicroRNA expression profile of human umbilical vein endothelial cells treated with Angiogenin (ANG)

Project description:We quantified differential microRNA (miRNA) expression in Human umbilical vein endothelial cells （HUVECs）response to Angiogenin (ANG) treatment.These data were used to determine which miRNAs are altered on ANG in Human umbilical vein endothelial cells.

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Effect of overexpression of miR-210-3p on gene expression of human umbilical vein endothelial cells in hypoxic conditions

Project description:To investigate machanism of miR-210-3p regulating angiogenic ability of human umbilical vein endothelial cells (HUVECs) in hypoxic conditions, we transfected miR-210-3p mimic to overexpress miR-210-3p in human umbilical vein endothelial cells. We than performed RNA sequencing of miR-210-3p mimic-transfected and control HUVECs under hypoxic conditions to evaluate the transcriptional changes in the miR-210-3p-overexpressing HUVECs.

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Hypoxia effect on human umbilical vein endothelial cells - gene and exon expression

Project description:Human umbilical vein endothelial cells (HUVECs) were incubated for 48 h under normoxic or hypoxic (1% oxygen) conditions. Changes in transcript and exon levels were analyzed.

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Expression data from Human Umbilical Vein Endothelial Cells

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DNA methylation profiles of human umbilical vein endothelial cells (HUVECs)

Project description:Replicative senescence has major impact on function and integrity of cell preparations. DNA-methylation (DNAm) changes are continuously acquired at specific CpG dinucleotides during in vitro culture. Such modifications can be used to estimate the state of cellular senescence for quality control of cell preparations. It is however unclear how senescence-associated DNAm changes are regulated and if they occur simultaneously throughout cell populations. In this study, we analyzed global DNAm profiles of human mesenchymal stem cells (MSCs) and human umbilical vein endothelial cells (HUVEC) to demonstrate that senescence-associated DNAm changes are overall similar in these different cell types. Subsequently, an Epigenetic-Senescence-Signature, based on six CpGs, was either analyzed by pyrosequencing or by barcoded bisulfite amplicon sequencing. There was a good correlation between predicted and real passage numbers in bulk populations of MSCs (R² = 0.67) and HUVECs (R² = 0.97).

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Microarray analysis of Replicative senescence and TNF-α induced senescence in Human umbilical vein endothelial cells (HUVECs)

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