Genomics

Dataset Information

0

HoxA13 gain-of-function in chicken embryo PSM progenitors.


ABSTRACT: Within a given vertebrate species, the total number of vertebrae in each anatomical domain is precisely defined and shows little variation among individuals. In contrast, this number can vary tremendously between different species, ranging from as few as six vertebrae in frogs to as many as several hundred in some snakes and fish. Segmental precursors of the vertebrae, called somites are produced sequentially in the embryo from the presomitic mesoderm (PSM), until a final number characteristic of the species, is reached. Here, we show in the chicken embryo that, by controlling the rate of axis elongation, Hox genes control the total number of somites generated by the embryo. We observed that activation of the most posterior Hox genes in somite precursors of the tail bud correlates with an abrupt slowing-down of the speed of axis elongation. We show that progressively more posterior Hox genes, which are collinearly activated in somitic precursors of the epiblast, repress Wnt activity with increasing strength. This leads to a graded repression of the Brachyury/T transcription factor, reducing mesoderm ingression and slowing down the elongation process. Due to the continuation of somite formation, the PSM, which is not fed with sufficient supply of new cells posteriorly, becomes progressively exhausted, ultimately leading to an arrest of segment formation. Our data provide a conceptual framework to explain how the cross-talk between the segmentation clock and the Hox clock accounts for the diversity of vertebral formulae across animal species.

ORGANISM(S): Gallus gallus

PROVIDER: GSE38107 | GEO | 2012/05/23

SECONDARY ACCESSION(S): PRJNA167365

REPOSITORIES: GEO

Similar Datasets

| E-GEOD-38107 | biostudies-arrayexpress
| E-MTAB-12539 | biostudies-arrayexpress
2023-11-09 | GSE244768 | GEO
| E-MTAB-6155 | biostudies-arrayexpress
2017-02-01 | GSE90876 | GEO
2019-10-09 | GSE87775 | GEO
2019-10-09 | GSE87774 | GEO
2022-09-01 | GSE185136 | GEO
2022-09-01 | GSE189195 | GEO
| E-MTAB-12511 | biostudies-arrayexpress