Dataset Information


Expression during deetioaltion under Rc in wt and phy mutant seedlings

ABSTRACT: Etiolated seedling response to one hour of continuous red light. Four-day-old dark-grown wild-type RLD and phyA101, phyB1 and phyAphyB mutant seedlings were transferred to Rc (680 nm, 8 mol m-2 s-1) for 1 h or retained in darkness as controls. Three different biological replicates of each treatment were grown separately and extracted, processed, and analyzed independently.RNA was extracted and subjected to expression analysis by using the Affymetrix ATH1 microarray containing approximately 22,000 Arabidopsis genes . Overall design: 3 dark and 3 1hr Rc samples ( biological replicates) were analyzed for each genotype

INSTRUMENT(S): [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array

SUBMITTER: james m tepperman  

PROVIDER: GSE3811 | GEO | 2006-05-15



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phyA dominates in transduction of red-light signals to rapidly responding genes at the initiation of Arabidopsis seedling de-etiolation.

Tepperman James M JM   Hwang Yong-Sic YS   Quail Peter H PH  

The Plant journal : for cell and molecular biology 20061031 5

Contrary to expectations based on the visible phenotypic behavior of seedlings undergoing de-etiolation in response to continuous red light (Rc), previous gene expression profiling showed that one or more of the five-membered phytochrome (phy) family of Arabidopsis, other than phyB, is predominantly responsible for transducing the Rc signals to light-responsive genes. To begin to identify which phys are involved, and to define potential primary targets of phy signaling, we have examined the geno  ...[more]

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