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Autologous pairs of cutaneous melanocyte and melanoma cell cultures

ABSTRACT: Early-passage (<10 passages) cultures of melanoma cells from metastatic lymph node lesions and normal adult melanocytes explanted in parallel from the adjacent, non-involved skin of 5 patients were compared by cDNA arrays. Differences between normal and neoplastic counterparts were then assessed upon adjustment for individual factors.

ORGANISM(S): Homo sapiens

PROVIDER: GSE38312 | GEO | 2012/09/28

SECONDARY ACCESSION(S): PRJNA169503

REPOSITORIES: GEO

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autologous pairs of cutaneous melanocyte and melanoma cell cultures

Project description:Early-passage (<10 passages) cultures of melanoma cells from metastatic lymph node lesions and normal adult melanocytes explanted in parallel from the adjacent, non-involved skin of 5 patients were compared by cDNA arrays. Differences between normal and neoplastic counterparts were then assessed upon adjustment for individual factors. cDNA expression profiles comparison between normal and neoplastic counterparts in a strictly autologous setting

2012-09-28 | E-GEOD-38312 | biostudies-arrayexpress

autologous pairs of cutaneous melanocyte and melanoma cell cultures

Project description: Not available

| S-ECPF-GEOD-38312 | biostudies-other

Large scale production of melanocyte derived from pluripotent stem cells for biotherapeutic approaches to treat skin pigmentation disorders

Project description:In this study, we successfully defined a highly efficient standardize method to derive hPSCs into a pure population of functional melanocytes. These cells can be maintain for more than 20 passages without observed any senescence. Transcriptomic anlaysis revealed a differentiation process with a logic gene expression profile following the kinetic of melanocyte embryonic development as previously described in mice. Our findings represent a fruitful ground that can serve as a database tool to decipher molecular signatures in normal and pathological conditions

2023-03-30 | GSE222639 | GEO

Expression data from human choroidal melanocyte cultures

Project description:Purpose: To determine to which extend inflammatory cytokines affect chemokine secretion by primary human choroidal melanocytes (HCMs), their capacity to attract monocytes and if HCMs are able to influence the proliferation of activated T cells. Methods: Primary cultures of HCMs were established from eyes of thirteen donors. HCMs were stimulated with IFN-γ and TNF-α or with supernatant from activated T-cells (T-cell conditioned media (TCM)). Gene expression analysis was performed using microarrays. Protein levels were quantified with ELISA or cytometric bead array. Supernatants of HCMs were assessed for the capability to attract monocytes in a trans-well plate. Proliferation of activated T-cells was assessed in a direct co-culture with HCMs by a [3H]-thymidine incorporation assay. Results: Stimulation of HCMs with TCM or IFN-γ and TNF-α resulted in increased expression and secretion of CXCL8, CXCL9, CXCL10, CXCL11, CCL2, CCL5 and intercellular adhesion molecule 1. Vascular endothelial growth factor and monocyte migration inhibitory factor were constitutively expressed without changes in response to pro-inflammatory cytokines. Supernatants derived from unstimulated cultures of ten HCM donors induced a high initial level of monocyte migration, which decreased upon stimulation with either TCM or IFN-γ and TNF-α. The supernatants from three HCM donors initially showed a low level of monocyte attraction which increased following exposure to pro-inflammatory cytokines. Direct co-culture of HCMs with T-cells resulted in inhibition of T-cell proliferation. Conclusion: These results show that normal and activated HCMs are immunologically active by secreting chemokines, and that HCMs are able to attract monocytes in addition to inhibit T-cell proliferation.

2016-12-21 | GSE70762 | GEO

iPS derived melanocyte precursor cells

Project description:We found distinct and self-renewal cell linages that could differentiate into melanocytes, named melanocyte precursor cells (MPCs) in which MPCs revealed drastically differntiation to authentic melanocytes by a small amount of GSK3β inhibitor in about seven days. Comparing MPCs ,iPSCs and melanocytes, we showed wnt sigal systems in differentiation to melanocytes.

2019-02-11 | GSE118227 | GEO

Expression data of primary melanocytes from aryl hydrocarbon deficient mice and corresponding wild-type C57BL/6 mice

Project description:[original Title] Comparison of expression data of primary murine melanocytes from aryl hydrocarbon deficient mice and corresponding wild-type C57BL/6 mice Melanin is produced exclusively by melanocytes and melanogenesis is the vital response to protect skin cells against Ultraviolet B (UVB)-induced DNA damage. The aryl hydrocarbon receptor (AhR) is a transcription factor, which may be involved in the physiological tanning response. Normal murine melanocytes express functional AhR. We tested gene expression in WT versus AhR-deficient mice primary murine melanocytes, isolated from the skin and cultivated for several passages.

2010-09-05 | GSE19411 | GEO

Gene expression data of iPS clones before melanocyte differentiation

Project description:We found distinct and self-renewal cell linages that could differentiate into melanocytes, named melanocyte precursor cells(MPCs) in which MPCs revealed drastically differntiation to authentic melanocytes by a small amount of GSK3β inhibitor in about seven days. Comparing MPCs ,iPSCs and melanocytes, we showed wnt sigal systems in differentiation to melanocytes.

2019-06-20 | GSE122981 | GEO

Expression data of primary melanocytes from aryl hydrocarbon deficient mice and corresponding wild-type C57BL/6 mice

2010-09-05 | E-GEOD-19411 | biostudies-arrayexpress

Chromatin-remodelling complex NURF is essential for differentiation of adult melanocyte stem cells

Project description:MIcrophthalmia-associated Transcription Factor (MITF) regulates melanocyte and melanoma physiology. ShRNA-mediated silencing of the NURF subunit BPTF revealed its essential role in several melanoma cell lines and in untransformed melanocytes in vitro. Comparative RNA-seq shows that MITF and BPTF co-regulate overlapping gene expression programs in cell lines in vitro. Somatic and specific inactivation of Bptf in developing murine melanoblasts in vivo shows that Bptf regulates their proliferation, migration and morphology. Once born, Bptf-mutant mice display premature greying where the second post-natal coat is white. This second coat is normally pigmented by differentiated melanocytes derived from the adult melanocyte stem cell (MSC) population that is stimulated to proliferate and differentiate at anagen. An MSC population is established and maintained throughout the life of the Bptf- mutant mice, but these MSCs are abnormal and at anagen, give rise to reduced numbers of transient amplifying cells (TACs) that do not express melanocyte markers and fail to differentiate into mature melanin producing melanocytes. MSCs display a transcriptionally repressed chromatin state and Bptf is essential for reactivation of the melanocyte gene expression program at anagen, the subsequent normal proliferation of TACs and their differentiation into mature melanocytes.

2015-10-07 | GSE72915 | GEO

Expression data from The human glioblastoma cell culture resource: validated cell models representing all molecular subtypes (transcript)

Project description:To explore the degree to which the glioma cell lines remained transcriptionally stable under diverse experimental conditions, we transplanted three different lines (U3020MG, U3047MG and U3065MG) intracranially to NOD-SCID mice; explanted the resulting tumors and cultured the cells for two passages, and then isolated RNA from the cell line prior to transplantation (U3020MG-p10, U3047MG-p7, U3065MG-p10), from the xenograft tumor and from the explanted cells.

2015-08-20 | GSE72218 | GEO

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