Mouse expression data of epididymal white adipose tissue at Zeitgeber time (ZT) 18 comparison control vs. Sleep restriction
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ABSTRACT:
ORGANISM(S): Mus musculus
PROVIDER: GSE38921 | GEO | 2015/06/15
SECONDARY ACCESSION(S): PRJNA169324
REPOSITORIES: GEO
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Project description:We hybridzed cRNA from epididymal white adipose tissue collected at ZT18 of control animals and TSR animals (TSR: these mice were sleep restricted for 6 hours every day by gentle handling for 5 consecutive days and killed on the last day at ZT18) mice used in this study were C57BL/6 control mice were compared to timed sleep restriction mice (TSR: these mice were sleep restricted for 6 hours every day by gentle handling for 5 consecutive days and killed on the last day at ZT18)
2015-06-15 | E-GEOD-38921 | biostudies-arrayexpress
Project description:The cone-rod homeobox gene (Crx) encodes Crx, a transcription factor selectively expressed in two cell types, retinal photoreceptors and the melatonin secreting pinealocytes of the pineal gland. In this report the role of Crx in regulating gene expression in the mammalian pineal gland was extended using Affymetrix GeneChip technology. Deletion of Crx results in broad modulation of the mouse pineal transcriptome, including a >2-fold downregulation of 543 genes and a >2-fold upregulation of 745 genes. In addition to Crx, there was a >10-fold downregulation of 13 other genes. Of special interest was the discovery of a link between Crx and the homeobox gene Hoxc4, which was upregulated ~20-fold in the Crx-/- pineal gland. Analysis of night and day expression of genes indicated that a set of 51 genes exhibited differential expression in control animals. Of these genes, only eight were also differentially expressed in Crx-/- animals. This group included Aanat, which encodes the enzyme that controls the daily rhythm in melatonin synthesis in the vertebrate pineal gland. Accordingly, Crx appears to be essential for the 24-hour rhythmic component of expression of some genes in the pineal gland. In the Crx-/- mouse pineal gland, 41 genes exhibited differential night/day expression that was not seen in control animals, suggesting that Crx may function to modulate rhythmic expression of these genes as well. Together, the results of this investigation indicate that Crx broadly modulates the pineal transcriptome, perhaps in part through suppressive effects on expression of the homeobox gene Hoxc4.
2012-01-01 | GSE24625 | GEO
Project description:The cone-rod homeobox gene (Crx) encodes Crx, a transcription factor selectively expressed in two cell types, retinal photoreceptors and the melatonin secreting pinealocytes of the pineal gland. In this report the role of Crx in regulating gene expression in the mammalian pineal gland was extended using Affymetrix GeneChip technology. Deletion of Crx results in broad modulation of the mouse pineal transcriptome, including a >2-fold downregulation of 543 genes and a >2-fold upregulation of 745 genes. In addition to Crx, there was a >10-fold downregulation of 13 other genes. Of special interest was the discovery of a link between Crx and the homeobox gene Hoxc4, which was upregulated ~20-fold in the Crx-/- pineal gland. Analysis of night and day expression of genes indicated that a set of 51 genes exhibited differential expression in control animals. Of these genes, only eight were also differentially expressed in Crx-/- animals. This group included Aanat, which encodes the enzyme that controls the daily rhythm in melatonin synthesis in the vertebrate pineal gland. Accordingly, Crx appears to be essential for the 24-hour rhythmic component of expression of some genes in the pineal gland. In the Crx-/- mouse pineal gland, 41 genes exhibited differential night/day expression that was not seen in control animals, suggesting that Crx may function to modulate rhythmic expression of these genes as well. Together, the results of this investigation indicate that Crx broadly modulates the pineal transcriptome, perhaps in part through suppressive effects on expression of the homeobox gene Hoxc4. Pineal glands from control (129sv) mice and Crx-/- mice were collected at ZT6 and ZT20 for RNA extraction and hybridization on Affymetrix mouse 430_2 chip. Each condition were performed as triplicats. SUPPLEMENTARY FILES: The GCOS signal intensity data were analyzed using ChipInspector (Genomatix) version 2.1. FDR= 0, p-value <0.05, cut off=1, region size = 300 bp and 4 and 5 significant probes. Log(2) fold change independently of time of day. Up regulated genes: genes which are up regulated in Crx-/- compared to the control; down regulated genes: genes which are down regulated in Crx-/- compared to the control.
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Project description:Gene expression in forebrain structures change during day and night depending on circadian and rest-activity cycles. Clock genes have been shown to be involved in the control of circadian and sleep-wake control. In this study, we aimed at studying the consequences of loss of SHARP1 (DEC2/BHLHE40) and SHARP2 (DEC1/BHLHE41) on cortical gene expression at rest (ZT4) and activity (ZT16) phases. We harvested cortex tissue from individual mice from WT and SHARP1/2 double null mutant mice at ZT4 and ZT16, prepared total RNA and subjected the corresponding samples (n=2 biological replicates per timepoint and genotype) to Affymetrix genechip analysis to assay the changes of gene expression.
2014-11-21 | E-GEOD-59941 | biostudies-arrayexpress
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2016-09-07 | E-GEOD-78215 | biostudies-arrayexpress
Project description:Molecular profiles in sleep and sleep deprivation in peripheral tissues using microarrays Time point study. Mice were sacrificed by cervical dislocaton following 3, 6, 9, and 12 h of total sleep deprivation (n = 8 or 9 at each time point). Deprivation was initiated at lights-on and performed through gentle handling.
2012-11-17 | E-GEOD-42324 | biostudies-arrayexpress
Project description:Molecular profiles in sleep and sleep deprivation in peripheral tissues using microarrays Time point study. Mice were sacrificed by cervical dislocaton following 3, 6, 9, and 12 h of total sleep deprivation (n = 8 or 9 at each time point). Deprivation was initiated at lights-on and performed through gentle handling.
2012-11-17 | E-GEOD-42323 | biostudies-arrayexpress
Project description:Galanin neurons at ventrolateral preoptic nuleus (VLPO) have been hypothesized to be sleep-active neurons. With the use of laser capture microdissection (LCM), we specifically isolated single galanin neurons, and our goal was to assess expression changes in VLPO galanin cells between sleeping mice and mice kept awake using gentle handling.
2020-08-27 | GSE132308 | GEO