Project description:The transcriptome pattern in blastocyst that developed from cumulus oocyte complexes matured in coculture with porcine luteal cells was investigated.
Project description:Comparative trascriptomic analysis between porcine early-blastocyst and Hatched blastocyst collected around day 5-6 direct comparison with dye-swap; two different arrays with 6 Samples each
Project description:<p>Melatonin (MT), a neurohormone synthesized and secreted primarily by the pineal gland, is of vital function to animal reproduction. However, the effect of MT treatment on porcine immature Sertoli cells (iSCs) remains unclear. Here, MT treatment (10 nM, 36 h) could elevate mitochondrial function and reduce oxidative stress, therefore inhibit apoptosis of porcine iSCs. Transcriptome profiling identified 39 differentially expressed genes (DEGs) (33 known and 6 novel) (MT vs Control), mainly involved in GO terms of steroid metabolic process, glutamine metabolic process, oxidoreductase activity and G protein coupled receptor binding, and KEGG pathways of steroid biogenesis, pyruvate metabolism, NF-kappa B and AMPK signal pathways. RT-qPCR validated 6 DEGs (Phgdh, Scd, Hmgcs1, Cytb, Pck2 and Sqle) induced by MT to be with the similar change trend to RNA-seq results. The protein level of HMGCS1 and the estradiol level were confirmed to be significantly decreased by MT (10 nM, 36 h) treatment of porcine iSCs. Inhibition of HMGCS1 in porcine iSCs could significantly reduce the level of estradiol. The levels of cholesterol content within cells and lactate in culture medium were unchanged by MT (10 nM, 36 h). 14 metabolites were significantly altered by MT (10 nM, 36 h) treatment of porcine iSCs. Which were involved in multiple vital metabolic pathways. Collectively, MT treatment of porcine iSCs could promote functions of porcine iSCs via modulating gene expression and metabolism.</p>
Project description:Early embryonic development is highly sensitive to both developmental stage and culture environment, and in vitro fertilization (IVF) is known to induce transcriptional and epigenetic alterations that may compromise embryo quality and developmental competence. To systematically characterize stage-specific and origin-dependent transcriptional differences, we performed RNA sequencing of porcine embryos derived in vivo and by IVF at key preimplantation stages. Transcriptomes were generated from in vivo–derived blastocysts and hatched blastocysts, IVF-derived blastocysts and hatched blastocysts, as well as IVF-derived 4-cell embryos. Comparative analyses enable the dissection of transcriptional programs associated with early cleavage, blastocyst formation, hatching, and culture-induced perturbations. This dataset provides a comprehensive resource for investigating developmental stage transitions, IVF-associated transcriptional dysregulation, and molecular determinants of embryo quality during porcine preimplantation development.