Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression analysis of Plasmodium falciparum 3D7 delta-PfPuf2 mutant

ABSTRACT: Investigation of whole genome gene expression level changes in Plasmodium falciparum 3D7 delta-PfPuf2 mutant, compared to the wild-type strain 3D7. The mutation engineered into this strain render tanslational control. The mutants analyzed in this study are further described in Miao J, Li J, Fan Q, Li X, Li X, Cui L.2010. The Puf-family RNA-binding protein PfPuf2 regulates sexual development and sex differentiation in the malaria parasite Plasmodium falciparum. J Cell Sci. 123(7):1039-49 (PMID 20197405).

ORGANISM(S): Plasmodium falciparum 3D7

PROVIDER: GSE39124 | GEO | 2012/07/06

SECONDARY ACCESSION(S): PRJNA170058

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Expression analysis of Plasmodium falciparum 3D7 delta-PfPuf2 mutant

Project description:Investigation of whole genome gene expression level changes in Plasmodium falciparum 3D7 delta-PfPuf2 mutant, compared to the wild-type strain 3D7. The mutation engineered into this strain render tanslational control. The mutants analyzed in this study are further described in Miao J, Li J, Fan Q, Li X, Li X, Cui L.2010. The Puf-family RNA-binding protein PfPuf2 regulates sexual development and sex differentiation in the malaria parasite Plasmodium falciparum. J Cell Sci. 123(7):1039-49 (PMID 20197405). A 12 chip study using total RNA recovered from six separate wild-type cultures of Plasmodium falciparum 3D7 at gametocyte stage III (three cultures) and stage V (three cultures) and six separate cultures of dalta PfPuf2 mutant at gametocyte stage III (three cultures) and stage V (three cultures). Each chip measures the expression level of 5,367 genes from Plasmodium falciparum 3D7 with 45-60 mer probes with two replicates on final array of 71618 probes.

2012-07-05 | E-GEOD-39124 | biostudies-arrayexpress

Next generation sequencing analysis of transcriptomes of Plasmodium falciparum male and female gametocytes

Project description:Investigation of overall expression level in Plasmodium falciparum male and female mature gametocytes, and detection of any transcriptional differences between male and female gametocytes. The Plasmodium falciparum parasite with green fluorescent protein (GFP) expression under the control of alpha tubulin II promoter facilitated the separation of male and female gametocyte. This engineered parasite strain in this study are further described in Miao J, Fan Q, Parker D, Li X, Li J, et al. (2013) Puf Mediates Translation Repression of Transmission-Blocking Vaccine Candidates in Malaria Parasites. PLoS Pathog 9(4): e1003268. doi: 10.1371/journal.ppat.1003268

2016-01-30 | GSE77398 | GEO

Expression analysis of Plasmodium falciparum male and female gametocytes

Project description:Investigation of whole genome gene expression level in Plasmodium falciparum male and female mature gametocytes, and detection of any transcriptional differences between male and female gametocytes. The Plasmodium falciparum parasite with green fluorescent protein (GFP) expression under the control of alpha tubulin II promoter facilitated the separation of male and female gametocyte. This engineered parasite strain in this study are further described in Miao J, Fan Q, Parker D, Li X, Li J, et al. (2013) Puf Mediates Translation Repression of Transmission-Blocking Vaccine Candidates in Malaria Parasites. PLoS Pathog 9(4): e1003268. doi: 10.1371/journal.ppat.1003268

2016-01-28 | GSE77316 | GEO

Expression analysis of Plasmodium falciparum with MYST overexpression

Project description:Investigation of genome-wide gene expression changes in Plasmodium falciparum overexpressing PfMYST compared to the wild-type strain 3D7. After introducing a single copy of the full-length PfMYST expression cassette into the parasite genome, parasites showed higher levels of H4-K5, -K8, and -K12 acetylation, a faster progression of intraerythrocytic developmental cycle (IDC), and shorter schizont development time (duration), which led to significantly fewer merozoites developed in mature schizonts than the control. The parasites with PfMYST overexpression substantially increased the resistance to DNA-damaging agents. These findings were published in Miao, Fan et al Mol Microbiol 2010 (PMID: 20807207). To understand the underlying mechanisms, we studied changes in the transcriptomes during the IDC by expression microarray.

2024-03-18 | GSE245596 | GEO

P.falciparum strain 3D7, SIR2 knockout

Project description:This experiment studies the effect of knocking out the transcriptional regulator SIR2 in 3D7 parasites. RNA was harvested from ring-stage parasites at 8 hours post-infection. Keywords = SIR2 Keywords = epigenetic regulation Keywords = antigenic variation Keywords = var Keywords = Plasmodium falciparum Keywords: other

2005-03-08 | GSE2369 | GEO

Time course transcriptome of Plasmodium falciparum parasite of 3D7 strain

Project description:The time course transcriptome were generated in Plasmodium falciparum parasite of 3D7 strain by collecting RNA samples every 2 hours during 48 hours of the full intraerythrocytic developmental cycle.

2020-10-20 | GSE149865 | GEO

High resolution time course transcriptome of Plasmodium falciparum parasite 3D7 strain by RNA-seq

Project description:The time course transcriptome of Plasmodium falciparum 3D7 parasite strain was generated by collecting RNA samples every 2 hours during full Intraerythrocytic Developmental Cycle.

2020-10-20 | GSE150484 | GEO

Plasmodium falciparum 3D7

Project description:Plasmodium falciparum 3D7 Genome sequencing

| PRJNA738256 | ENA

Plasmodium falciparum 3D7

Project description:Plasmodium falciparum 3D7 Genome sequencing

| PRJNA170465 | ENA

Comparative Genomic Hybridizaions of 3D7∆PfSir2a and NF54 compared to reference strain 3D7

Project description:Antigenic variation in Plasmodium falciparum is achieved by transcriptional switching amongst polymorphic var genes, enforced by epigenetic modification of chromatin. Histone-modifying ‘sirtuin’ enzymes PfSir2a and PfSir2b have been implicated in this process. We examined the effect of genetic disruption of sirtuins on var gene expression. Comparative Genomic Hybridization profile indicate that loss of PfSir2a in 3D7 resulted in strikingly rearranged chromosomes.

2014-10-02 | GSE61940 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data