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Differential methylation in discrete developmental stages of the parasitic nematode Trichinella spiralis

ABSTRACT: We present the first study to confirm the existence of DNA methylation in the parasitic nematode Trichinella spiralis, and we characterize the methylomes of the three life-cycle stages of this food-borne infectious human pathogen. We observe a drastic increase in DNA methylation during the transition from the new born to mature stage, and we further identify parasitism-related genes that show changes in DNA methylation status between life cycle stages. We also evaluated differential gene expression among the three life stages using Illumina HiSeq 2000 RNA-seq technology.

ORGANISM(S): Trichinella spiralis

PROVIDER: GSE39328 | GEO | 2012/09/28

SECONDARY ACCESSION(S): PRJNA170655

REPOSITORIES: GEO

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Differential methylation in discrete developmental stages of the parasitic nematode Trichinella spiralis

Project description:We describe the first comprehensive study confirming the existence of DNA methylation, characterising the methylomes of three life stages of the food-borne agent of human trichinellosis, Trichinella spiralis. We further identify sets of genes where the DNA methylation status varied between thedevelopmental stages that are closely related to the parasitism of the organism. Examination of DNA methylation status in three life stages (Adult, muscle larve, new born larve) of Trchinella Spiralis using MethylC-seq.

2012-09-28 | E-GEOD-39328 | biostudies-arrayexpress

Global gene expression analysis of the zoonotic parasite Trichinella spiralis revealed novel genes in host parasite interaction

Project description:Trichinellosis of human and other mammals was caused through the ingestion of the parasite，Trichinella spiralis，contaminated meat. It is a typical zoonotic disease that affects more than 10 million people world-wide. Parasites of Trichinella genus are unique intracellular pathogens. Adult Trichinella parasites directly release newborn larvae which invade striated muscle cells and causes diseases. In this study, we profiled the global transcriptome in the three developmental stages of T. spiralis. The transcriptomic analysis revealed the global gene expression patterns from newborn larval stage through muscle larval stage to adults. Thousands of genes with stage-specific transcriptional patterns were described and novel genes involving host-parasite interaction were identified. More than 45% of the protein-coding genes showed evidence of transcription from both sense and antisense strands which suggests the importance of RNA-mediated gene regulation in the parasite. This study presents a first deep analysis of the transcriptome of T. spiralis, providing insight information of the parasite biology.

2013-06-18 | GSE39151 | GEO

Trichinella spiralis

Project description:Differential methylation in discrete developmental stages of the parasitic nematode Trichinella spiralis

| PRJNA170655 | ENA

Global gene expression analysis of the zoonotic parasite Trichinella spiralis revealed novel genes in host parasite interaction

Project description:Trichinellosis of human and other mammals was caused through the ingestion of the parasiteM-oM-<M-^LTrichinella spiralisM-oM-<M-^Lcontaminated meat. It is a typical zoonotic disease that affects more than 10 million people world-wide. Parasites of Trichinella genus are unique intracellular pathogens. Adult Trichinella parasites directly release newborn larvae which invade striated muscle cells and causes diseases. In this study, we profiled the global transcriptome in the three developmental stages of T. spiralis. The transcriptomic analysis revealed the global gene expression patterns from newborn larval stage through muscle larval stage to adults. Thousands of genes with stage-specific transcriptional patterns were described and novel genes involving host-parasite interaction were identified. More than 45% of the protein-coding genes showed evidence of transcription from both sense and antisense strands which suggests the importance of RNA-mediated gene regulation in the parasite. This study presents a first deep analysis of the transcriptome of T. spiralis, providing insight information of the parasite biology. Messenger RNA from three developmental stages of T. spiralis was selectively purified from total RNA using oligo-(dT) conjugated magnetic beads. Complementary DNA (cDNA) was synthesized guided by oligo-(dT) as a primer.

2013-06-18 | E-GEOD-39151 | biostudies-arrayexpress

Expression data from mouse intestinal epithelial cells infected with Trichinella spiralis

Project description:Trichinella spiralis is a highly destructive parasitic nematode that invades and destroys intestinal epithelial cells, injures many different tissues during its migratory phase, and occupies and transforms myotubes during the final phase of its life cycle. Mice deficient in the IL-1 family receptor for the DAMP, IL-33 (called ST2), display reduced intestinal Th2 responses and impaired mast cell activation. IL-33 was constitutively expressed in intestinal epithelial cells, where it became concentrated in nuclei within 2 days of infection. Nuclear localization was an innate response to infection that occurred in intestinal regions where worms were actively migrating. We isolated intestinal epithelial cells from uninfected mice (cytoplasmic IL-33) and mice at 2 days post-infection (nuclear IL-33) to compare global expression profiles. We used microarrays to characterize the global gene expression that occurs in intestinal epithelial cells following T. spiralis-induced nuclear translocation of IL-33.

2015-03-28 | GSE67365 | GEO

Expression data from mouse intestinal epithelial cells infected with Trichinella spiralis

2015-03-28 | E-GEOD-67365 | biostudies-arrayexpress

Trichinella spiralis

Project description:Transcriptome sequencing of Trichinella spiralis larvae

| PRJNA510020 | ENA

12 single-base resolution methylomes of three life stages using WGBS

Project description:As the most studied type of epigenetic modifications found in many taxa, DNA methylation has been confirmed to play a crucial role in transposon silencing, transcriptional regulation and thus phenotypic variation, as well as rapid adaption to changing environments. To fully understand the methylome variation in Trichinella, here, we report 12 single-base resolution methylomes of three life stages using WGBS. By comparative epigenomics, we observe that the methylome variation in Trichinella is significantly divergent and host-related. By comparative epigenomics, we observe that the methylome variation in Trichinella is significantly divergent and host-related. By comparing DNA methylation patterns between different host classes of species, we found a fraction of parasitism-related genes under epigenetic regulation, such as G-protein-coupled receptor, DNaseII and ligand-gated chloride channel. Moreover, we also reveal associations between methylation divergence and genetic basis, including nucleotide variant and structural variation.

2021-06-01 | GSE139486 | GEO

Trichinella spiralis strain:ISS 195

Project description:Trichinella spiralis genome sequencing project

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WGS data to conduct phylogenetic analysis in Trichinella

Project description:To reveal the correlation between epigenomic diversity and genomic SNVs, we first identified SNPs between T4 with other eleven Trichinella species. In total, we obtained 5,395,250 common SNVs across the twelve Trichinella species.

2021-06-01 | GSE169404 | GEO

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