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Effects of breaking up prolonged sitting on skeletal muscle gene expression

ABSTRACT: Breaking up prolonged sitting has been shown to be beneficially associated with cardio-metabolic risk markers in both observational and intervention studies. We aimed to define the acute transcriptional events induced in skeletal muscle by breaks in sedentary time. Overweight/obese adults participated in a randomized three-period, three-treatment cross-over trial in an acute setting (5 hours): seated position with no activity, seated with 2-minute bouts of light- or moderate-intensity treadmill walking every 20 minutes. Vastus lateralis biopsies were obtained in 8 of the 19 participants after each treatment and gene expression examined using microarrays validated with real-time qPCR.

ORGANISM(S): Homo sapiens

PROVIDER: GSE39576 | GEO | 2012/07/25

SECONDARY ACCESSION(S): PRJNA171152

REPOSITORIES: GEO

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Effects of breaking up prolonged sitting on skeletal muscle gene expression

Project description:Breaking up prolonged sitting has been shown to be beneficially associated with cardio-metabolic risk markers in both observational and intervention studies. We aimed to define the acute transcriptional events induced in skeletal muscle by breaks in sedentary time. Overweight/obese adults participated in a randomized three-period, three-treatment cross-over trial in an acute setting (5 hours): seated position with no activity, seated with 2-minute bouts of light- or moderate-intensity treadmill walking every 20 minutes. Vastus lateralis biopsies were obtained in 8 of the 19 participants after each treatment and gene expression examined using microarrays validated with real-time qPCR. Total RNA was extracted from vastus lateralis at the 3 different experimental condition

2012-07-24 | E-GEOD-39576 | biostudies-arrayexpress

Acute effects of active breaks during prolonged sitting on subcutaneous adipose tissue gene expression

Project description:Breaking up prolonged periods of time spent sitting has a range of beneficial impacts on cardiometabolic risk biomarkers. The molecular mechanisms include regulation of skeletal muscle gene and protein expression controlling metabolic, inflammatory and cell development pathways. An active communication network exists between adipose and muscle tissue, but the effect of active breaks in prolonged sitting on adipose tissue have yet to be investigated. This study characterised the acute transcriptional events induced in adipose tissue by regular active breaks during prolonged sitting. In a subset of 8 overweight/obese adults participating in an acute randomised three-intervention crossover trial, subcutaneous adipose tissue biopsies were obtained after each condition. The three experimental conditions were conducted in the postprandial state and included: i) prolonged uninterrupted sitting; or prolonged sitting interrupted with 2-minute bouts of ii) light- or iii) moderate-intensity treadmill walking every 20 minutes. Microarrays identified 36 differentially expressed genes between the three conditions (fold change≥0.5 in either direction; p<0.05). Pathway analysis indicated that breaking up of prolonged sitting led to differential regulation of adipose tissue metabolic networks and inflammatory pathways, increased insulin signalling, increased adipocyte turnover, and facilitated cross-talk between adipose tissue and other organs. This study provides insight into the adipose tissue regulatory systems and transcriptional processes that contribute to the physiological benefits of interrupting prolonged sitting.

2018-06-13 | GSE115645 | GEO

Transcription profiling of rat soleus during acute physical inactivity and walking

Project description:Global gene expression of the rat soleus muscle was assessed with microarrays after 12 hrs of physical inactivity and also after 12 hours of physical inactivity immediately followed by 2 hrs treadmill walking and compared to normally caged rats with voluntary standing and intermittent cage movements. Experiment Overall Design: Rats were either prevented from standing on the their hindlimbs for 12 hrs or were prevented from standing on the their hindlimbs for 12 hrs and then immediately engaged in 2 hours treadmill walking and compared to caged rats with normal standing/ambulatory activity. RNA was extracted from soleus muscles and each array represents the pooling of the RNA from the soleus of 8-10 different rats.

2008-06-13 | E-GEOD-5215 | biostudies-arrayexpress

Expression data from rat soleus during acute physical inactivity and walking

2006-07-04 | GSE5215 | GEO

Serum proteomics reveals a sexually dimorphic effect of vitamin D status correction on proteins of the blood coagulation pathway among overweight adults

Project description:Background: An important feature to vitamin D physiology is its gender dependence. The aim of this study was to examine whether vitamin D exerts a sexually dimorphic effect on the blood coagulation pathway among adults with overweight. Methods: This study compared the serum proteomic profiles of age and BMI-matched males (n=26) and pre-menopausal females (n=24) with overweight that attained vitamin D sufficiency after a 12-month intervention. Unprocessed serum was subjected to depletion-free, quantitative proteomic analysis using our previously published methodology. Results. A total of 1,841 proteins were profiled (p < 0.05). The analysed proteins vitamin-K dependent protein C, von Willebrand factor, fibrinogen gamma chain and multimerin-1 were ELISA validated to be differentially affected between genders by vitamin D status improvement. Conclusions: Vitamin D optimization exhibits a sexually dimorphic effect on the blood coagulation pathway among adults with overweight. This gender specific vitamin D effect should be taken into consideration in the design and interpretation of vitamin D observational and intervention studies.

2016-10-13 | PXD003663 | Pride

Short-Term Adaptations in Skeletal Muscle Mitochondrial Oxidative Capacity and Metabolic pathways to Breaking Up Sedentary Behaviors with Short-Frequent Bouts of Physical Activity Compared to a Matched Daily Single-Continuous Bout in Obese Adults

Project description:We compared the short-term (4-day) adaptations in skeletal muscle mitochondrial function and gene expression between short-frequent bouts of physical activity and time matched single-continuous physical activity.

2022-02-16 | GSE186715 | GEO

Scottish Colorectal Cancer Vitamin D Intervention Study (SCoViDS) [RNA-seq]

Project description:Controversy surrounds whether vitamin D reduces colorectal cancer (CRC) risk, and/or beneficially impacts on CRC survival. We sought to delineate the transcriptomic in vivo response of human colorectal epithelium to vitamin D supplementation, define inter-subject variability and develop a predictive biomarker of response consistent with anti-tumour effects. Methodology Blood and rectal normal mucosa (NM) were sampled (176 subjects) and correlation sought between circulating vitamin D (25-OHD) level and NM gene expression (HT12 microarray) in the observational study. Oral vitamin D (3200IU/day) supplements were administered to 50 participants in an intervention study. Blood and NM (sigmoidoscopic biopsy) were sampled after 12 weeks. Transcriptomic analysis (HT12 and RNAseq) of post-treatment biopsies assessed enrichment for candidate genes and GO processes prioritised in the observational study. We identified transcriptomic changes in rectum that are consistent with anti-tumour responses. We then sought blood biomarkers predicting this response using receiver-operator curves and calculation of C-statistic. An independent expression dataset (BEST-D trial) was then used to validate candidate blood biomarkers of vitamin D response.

2021-06-22 | GSE157982 | GEO

The proteome of acute muscle pain: Observations from acute hypertonic saline induced pain in humans

Project description:Despite widespread use of experimental acute pain models to mimic chronic pathologies, little exploration has been undertaken on the acute pain proteome in humans. We re-solved to explore molecular alterations evoked by acute bouts of hypertonic saline (HS) induced muscle pain and map the spread of evoked mechanical hyperalgesia. This study used a 2-cohort design in healthy human participants. Cohort one (n=16) underwent in-termittent blood sampling concurrent with intramuscular HS (5%) infusion to allow for the discovery of the proteomic and cytokine profile of acute muscle pain. Cohort two (n=10) underwent bilateral sensory testing during HS infusion, to map the spread of evoked mechanical hyperalgesia. Molecular analysis in cohort one revealed a diffuse ar-ray of proteins and cytokines showing altered expression in response to acute muscle pain. Particularly these alterations were linked to metabolism and immune response pathways suggestive of systemic effects of acute pain. Intriguingly, cohort two revealed a significant mechanical hyperalgesia which emerged in a diffuse pattern over the ipsilat-eral limb to HS-infusion. However, despite evidence of systemic molecular alterations, no such mechanical hypersensitivity was observed in the contralateral limb. This study demonstrates systemic molecular alterations resultant from acute bouts of HS-induced muscle pain with accompanying sensory interactions.

2026-01-05 | PXD070131 | Pride

Rattus norvegicus

Project description:Expression data from rat soleus during acute physical inactivity and walking

| PRJNA96509 | ENA

Exercise intervention during pregnancy induces promoter methylation alterations in maternal blood and cord blood

Project description:Maternal blood, as well as umbilical cord blood samples, were collected and DNA methylation levels were determined by Illumina MethylationEPIC microarray. Methods: Twenty-four subjects were chosen from a previous clinical study. Overweight/obese pregnant women (body mass index ≥24kg/m2) who had an uncomplicated pregnancy at <12+6 weeks of gestation were randomly allocated to either an exercise or a control group. Patients allocated to the exercise group performed 3 exercise bouts per week (at least 30 min/session with a rating of perceived exertion between 12-14) via a cycling program that was initiated within 3 days of randomization until 37 weeks of gestation. Patients allocated to the control group continued their usual daily activities. Maternal blood, as well as umbilical cord blood samples, were collected and DNA methylation levels were determined by Illumina MethylationEPIC microarray.

2024-04-16 | GSE172208 | GEO

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