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Action of microgravity on root development-Action of microgravity on root development

ABSTRACT: au11-03_gravite - action of microgravity on root development - Action of microgravity on root development - Arabidopsis were grown on horizontal or vertical clinostat for 4, 8 or 12 days. Seedlings on horizontal clinostat were in simulated microgravity and seedlings on vertical clinostat are considered as a control. Comparison was made between plants grown on simulated microgravitry and vertical position.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE39631 | GEO | 2012/08/01

SECONDARY ACCESSION(S): PRJNA171302

REPOSITORIES: GEO

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action of microgravity on root development-Action of microgravity on root development

Project description:au11-03_gravite - action of microgravity on root development - Action of microgravity on root development - Arabidopsis were grown on horizontal or vertical clinostat for 4, 8 or 12 days. Seedlings on horizontal clinostat were in simulated microgravity and seedlings on vertical clinostat are considered as a control. Comparison was made between plants grown on simulated microgravitry and vertical position. 6 dye-swap - treated vs untreated comparison

2012-07-31 | E-GEOD-39631 | biostudies-arrayexpress

Time-resolved transcript, protein and metabolite profiling of Arabidopsis roots in simulated microgravity

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Changes in nuclear shape and gene expression in response to simulated microgravity are LINC complex-dependent

Project description:Microgravity is known to affect the organization of the cytoskeleton, cell and nuclear morphology and to elicit differential expression of genes associated with the cytoskeleton, focal adhesions and the extracellular matrix. Although the nucleus is mechanically connected to the cytoskeleton through the LInker of Nucleoskeleton and Cytoskeleton (LINC) complex, the role of this group of proteins in these responses to microgravity has yet to be defined. Therefore, we used simulated microgravity achieved by growing cells on a 3d clinostat to investigate whether the LINC complex acts to mediate responses to the microgravity environment. We show that nuclear shape and differential gene expression are both responsive to simulated microgravity in a LINC-dependent manner and that this response changes with the duration of exposure to simulated microgravity. These LINC-dependent genes likely represent elements normally regulated by the mechanical forces imposed by gravity on Earth.

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Effect of simulated microgravity on E. coli K12 MG1655 growth and gene expression

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