Transcriptomics

Dataset Information

20

Contribution of unique genes by individual cell lines to Universal Mouse Reference RNA


ABSTRACT: RNA from 11 individual mouse cell lines were reverse-transcribed to cDNA, labeled with Cy5 and cohybridized with Cy3-labeled UMRR onto 7,500-spot mouse oligo microarrays (UNC). The data was analyzed using GeneTraffic software. 300-1000 spots out of 8,000 were flagged on each microarray and excluded from further analysis. Spots with hybridization signals in Cy5 channel higher than 1000 and with Cy5/Cy3 ratio greater than 2 were collected and the number of spots with these characteristics on only one microarray was determined. A reference experiment design type is where all samples are compared to a common reference. Keywords: reference_design Overall design: Computed

INSTRUMENT(S): CGEN:Mm010706

ORGANISM(S): Mus musculus  

SUBMITTER: Stanford Microarray Database   

PROVIDER: GSE3974 | GEO | 2006-01-05

SECONDARY ACCESSION(S): PRJNA104879

REPOSITORIES: GEO

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