Project description:Profiling of miRNA expression in mouse SHAM kidneys 9–12 weeks old male C57BL/6 mice were purchased from Charles River. Mice underwent anesthesia by intraperitoneal injection of pentobarbital (50 mg/kg body weight) and were subjected to Unilateral Ureteral Obstruction (UUO). After standard laparotomy, the left proximal ureter was exposed and ligated with 4-0 silk at two points. The sham operation consisted of a similar identification of the left ureter without ligation. Treated (S numbers) and non-treated (C numbers) mice miRNA expressions were then compared.
Project description:RNA-sequencing of mouse left kidneys for which the left ureter was obstructed (UUO) or not obstructed (sham) animals, isolated 7 days after the surgery in normal mice and mice in which the Kdm6a gene was deleted from tubule epithelial cells
Project description:RNA-sequencing of mouse left kidneys where the left ureter was obstructed (UUO) or not obstructed (sham), isolated 14 days after the surgery in C57 mice treated with either istradefylline or vehicle.
Project description:RNA-sequencing of mouse left kidneys for which the left ureter was obstructed (UUO) or not obstructed (sham) animals, isolated 14 days after the surgery