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Transcriptional profiling of gastric epithelial cells infected with wild type or arginase-deficient Helicobacter pylori

ABSTRACT: We studied the differential gene expression in the gastric cell line AGS infected with wild type (WT) or arginase gene mutant (rocF) Helicobacter pylori (Hp). We found that the presence of the gene regulates the inflammatory responses in terms of IL8 and MIP-1B. We conclude that arginase may be involved in regulating inflammatory responses which improves the chances of survival against the human immune response.

ORGANISM(S): Homo sapiens

PROVIDER: GSE39919 | GEO | 2013/06/15

SECONDARY ACCESSION(S): PRJNA172026

REPOSITORIES: GEO

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Transcriptional profiling of gastric epithelial cells infected with wild type or arginase-deficient Helicobacter pylori

Project description:We studied the differential gene expression in the gastric cell line AGS infected with wild type (WT) or arginase gene mutant (rocF) Helicobacter pylori (Hp). We found that the presence of the gene regulates the inflammatory responses in terms of IL8 and MIP-1B. We conclude that arginase may be involved in regulating inflammatory responses which improves the chances of survival against the human immune response. AGS cells were infected for 16 hours with WT, the rocF, or a complemented H. pylori strain (rocF+). The RNA was extracted with TRIzol, treated with DNAse I (both from invitrogen). cRNA was made with the Illumina TotalPrep RNA amplification Kit (ambion AMIL1791).

2013-06-15 | E-GEOD-39919 | biostudies-arrayexpress

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Project description:Persistent colonization of the gastric mucosa by Helicobacter pylori (Hp) elicits chronic inflammation and aberrant epithelial cell proliferation, which increases the risk of gastric cancer. We examined the ability of microRNAs to modulate gastric cell proliferation in response to persistent Hp infection and found that epigenetic silencing of miR-210 plays a key role in gastric disease progression. Importantly, DNA methylation of the miR-210 gene was increased in Hp-positive human gastric biopsies as compared to Hp-negative controls. Moreover silencing of miR-210 in gastric epithelial cells promoted proliferation. We identified STMN1 and DIMT1 as miR-210 target genes and demonstrated that inhibition of miR-210 expression augmented cell proliferation by activating STMN1 and DIMT1. Together, our results highlight inflammation-induced epigenetic silencing of miR-210 as a mechanism of induction of chronic gastric diseases, including cancer, during Hp infection. To identify miR-210 targets in gastric cells, whole transcriptome analysis of AGS and MKN45 cells transfected with pre-miR-210 was conducted using Affymetrix GeneChip Human Genome U133 Plus 2.0 Array.

2014-09-04 | E-GEOD-58004 | biostudies-arrayexpress

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Project description:Macrophages are important cells involved in infections responses. They are mediators of gastritis in acute Helicobacter pylori (Hp) infection. After macrophages infection with Hp one of the most up-regulated protein is CD300E. To identify effects of CD300E activation in macrophages an agonistic antibody was used to activate CD300E and effects on gene expression were monitored.

2018-02-22 | GSE98639 | GEO

The expression of miRNAs is influenced by the infection of Helicobacter Pylori

Project description:Macrophages are important cells involved in infections responses. They are mediators of gastritis in acute Helicobacter pylori (Hp) Infection and to identify how Hp affects macrophages response to infection we measured miRNA expression after macrophages Hp infection.

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Transcriptome analysis of AGS cells infected with Helicobacter pylori P12

Project description:Transcriptome analysis of AGS cells infected with Helicobacter pylori P12

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Genomic Analyses of early-stage diffuse-type gastric cancer lacking Helicobacter pylori infection

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Project description:In this study, we purified and characterized two types of H.pylori OMVs (standard strain NCTC11637 and clinical strain Hp-400) and analyzed the proteome using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS).

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