Genomics

Dataset Information

42

MicroRNA-449a Enhances Radiosensitivity in Lung Adenocarcinoma Cells


ABSTRACT: Twenty-five miRNAs were identified as having differential expression post-irradiation in CL1-0 or CL1-5 cells. Among these miRNAs, miR-449a, which was down-regulated in CL1-0 cells at 24 h after irradiation, was chosen for further investigation. Overexpression of miR-449a in CL1-0 cells effectively increased irradiation-induced DNA damage and apoptosis, altered the cell cycle distribution and eventually led to sensitization of CL1-0 to irradiation. MiR-449a might be a novel radiosensitizer for clinical applications. Overall design: Two lung adenocarcinoma cell lines (CL1-0 and CL1-5) with different metastatic ability and radiosensitivity were used. In order to understand the regulatory mechanisms of differential radiosensitivity in these isogenic tumor cells, both CL1-0 and CL1-5 were treated with 10 Gy radiation, and were harvested respectively at 0, 1, 4, and 24 h after radiation exposure. The changes in expression of miRNA upon irradiation were examined using Illumina Human microRNA BeadChips.

INSTRUMENT(S): Illumina Human v2 MicroRNA expression beadchip

ORGANISM(S): Homo sapiens  

SUBMITTER: Mong-Hsun Tsai  

PROVIDER: GSE40602 | GEO |

SECONDARY ACCESSION(S): PRJNA174365

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
GSE40602_RAW.tar Raw
GSE40602_non-normalized.txt.gz Txt
filelist.txt Txt
Items per page:
1 - 3 of 3

Similar Datasets

2013-01-01 | S-EPMC3629161 | BioStudies
2012-12-30 | E-GEOD-40602 | ArrayExpress
2016-01-01 | S-EPMC4890029 | BioStudies
2017-01-01 | S-EPMC6966029 | BioStudies
2015-01-01 | S-EPMC4741479 | BioStudies
2020-01-01 | S-EPMC7532503 | BioStudies
1000-01-01 | S-EPMC5833763 | BioStudies
2019-01-01 | S-EPMC6770036 | BioStudies
1000-01-01 | S-EPMC4435581 | BioStudies
1000-01-01 | S-EPMC4252025 | BioStudies