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Molecular profiling of activated neurons by phosphorylated ribosome capture [Illumina BeadArray]

ABSTRACT: We report that phosphorylated ribosomes can be immunoprecipitated from mouse brain homogenates, enriching for mRNAs that were selectively expressed in activated neurons

ORGANISM(S): Mus musculus

PROVIDER: GSE40994 | GEO | 2012/09/20

SECONDARY ACCESSION(S): PRJNA175511

REPOSITORIES: GEO

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Molecular profiling of activated neurons by phosphorylated ribosome capture [Illumina BeadArray]

Project description:We report that phosphorylated ribosomes can be immunoprecipitated from mouse brain homogenates, enriching for mRNAs that were selectively expressed in activated neurons Mice were given an injection of concentrated salt solution (2M NaCl, 325 uL) or PBS, and then sacrificed 2h later and hypothalami dissected. Tissue homogenates were prepared from hypothalamus and phosphorylated ribosomes immunoprecipitated using pS6 244/247 antibodies. The immunoprecipitated RNA and total RNA from both control and salt treated animals were then purified and analyzed by Illumina Microarray.

2012-09-20 | E-GEOD-40994 | biostudies-arrayexpress

Molecular profiling of activated neurons by phosphorylated ribosome capture [RNA-Seq]

Project description:We report that phosphorylated ribosomes can be immunoprecipitated from mouse brain homogenates, resulting in enrichment of transcripts expressed in activated neurons. Mice were either injected with a concentrated salt solution or vehicle, hypothalami dissected, and phosphorylated ribosomes immunoprecipitated. RNA was sequenced from the input and IP for each condition (4 samples total).

2012-09-20 | E-GEOD-40969 | biostudies-arrayexpress

Molecular profiling of activated neurons by phosphorylated ribosome capture [RNA-Seq]

Project description:We report that phosphorylated ribosomes can be immunoprecipitated from mouse brain homogenates, resulting in enrichment of transcripts expressed in activated neurons.

2012-09-20 | GSE40969 | GEO

Molecular profiling of activated neurons by phosphorylated ribosome capture

Project description:This SuperSeries is composed of the following subset Series: GSE40969: Molecular profiling of activated neurons by phosphorylated ribosome capture [RNA-Seq] GSE40994: Molecular profiling of activated neurons by phosphorylated ribosome capture [Illumina BeadArray] Refer to individual Series

2012-09-20 | E-GEOD-40995 | biostudies-arrayexpress