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Transcriptional profiling of hypoxic cells and identification of HEXIM1-dependent genes.

ABSTRACT: To identify primary response genes (PRGs) that were differently expressed within 1 hr of hypoxia exposure, we performed global transcriptional profiling using the Agilent Technologies SurePrint G3 Human GE Microarray . To identify Hexim1-dependent genes, we compare profiles of hypoxia PRGs in cells transfected with control or Hexim1 targeting double-strand siRNA

ORGANISM(S): Homo sapiens

PROVIDER: GSE41023 | GEO | 2012/09/21

SECONDARY ACCESSION(S): PRJNA175557

REPOSITORIES: GEO

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Transcriptional profiling of hypoxic cells and identification of HEXIM1-dependent genes.

Project description:To identify primary response genes (PRGs) that were differently expressed within 1 hr of hypoxia exposure, we performed global transcriptional profiling using the Agilent Technologies SurePrint G3 Human GE Microarray . To identify Hexim1-dependent genes, we compare profiles of hypoxia PRGs in cells transfected with control or Hexim1 targeting double-strand siRNA HeLa cells were transfected with control and HEXIM1-targeted double-strand siRNA and 48 hr after treated with IL-1beta in combination with normoxia or hypoxia (5% CO2, 0.5% O2, balanced with N2) O for 1 hr.

2012-09-21 | E-GEOD-41023 | biostudies-arrayexpress

Effect of a DP1 agonist BW245C on concanavalin A-induced hepatitis

Project description:To elucidate the effect of a prostaglandin D2 receptor DP1 agonist BW245C on concanavalin A (ConA) hepatitis, we performed DNA microarray using SurePrint G3 Mouse GE 8x60K Microarray. Mice were treated with ConA for 3 or 24 hr with vehicle or BW245C . Livers were collected, homogenized, and subjected to total RNA extraction.

2015-06-01 | GSE45413 | GEO

Gene expression analysis of stimulated primary ASM cells [mRNA]

Project description:mRNA expression was determined using the Agilent-G4851A SurePrint G3 Human GE 8x60K Microarray

2014-05-28 | GSE57764 | GEO

Expression Profiles of SUM149 cell treated by ACY1215

Project description:Expression Profiles of SUM149 cell treated by ACY1215 at 0h, 3h, 6h and 12h by Agilent SurePrint G3 Human GE v3 8x60K.

2016-08-23 | GSE72344 | GEO

Transcriptional Profiling of Hypoxic Neural Stem Cells to Identify Novel Regulators of Neural Stem Cell Biology

Project description:Neural stem cells reside in a hypoxic microenvironment within the brain. However, the crucial transcription factors that regulate neural stem cell biology under physiologic hypoxia are poorly understood. Here, we have performed microarray analysis of hypoxic versus normoxic neural stem cells with the aim of identifying pathways and transcription factors that are activated under oxygen concentrations mimicking normal brain tissue microenvironment. To identify the molecular mechanisms mediating the effect of low oxygen levels on NSC biology, we profiled the global effect of sustained, physiologic hypoxia on NSC gene expression using an unbiased approach by genome-wide microarray analysis. NSC were isolated from E13.5 mouse embryos (OF-1 strain) as previously described (Johe et al., 1996). After isolation, cells were immediately cultured at 37°C, 5% CO2 and either 5% or atmospheric (≈21%) oxygen and maintained in these conditions for 2-3 passages (minimum of 10 days). Then, total RNA was extracted, labeled and hybridized in a SurePrint G3 Mouse GE 8x60k array (Agilent Technologies). Four independent experiments were performed using different mouse donors for each experiment.

2015-07-30 | E-GEOD-68572 | biostudies-arrayexpress

Sham vs.BCAS microarray for brain

Project description:The Agilent SurePrint G3 Mouse GE V2.0 Microarray was used in this experiment to analyze data of the 6 samples. Goal was to determine the differential genes of Sham and Bilateral carotid artery stenosis (BCAS).

2023-05-10 | GSE220106 | GEO

Large scale transcriptomic characterization of mice blood inflammation during Acute Lung Injury (PBMC)

Project description:ARN sample of PBMC from mouse were obtened at 6 times points: 1h, 1h30, 3h, 4h, 18H, 24h, after injection of oleic acid or physiological serum PBMC total RNAs from mouse injected with acid oleic or physiological serum were profiled after hybridization with Agilent SurePrint G3 Mouse GE 8x60K Microarray to identify genes with differential expression

2015-01-01 | GSE52684 | GEO

Large scale transcriptomic characterization of mice brain inflammation during Acute Lung Injury (brain)

Project description:ARN sample of brain from mouse were obtened at 6 times points: 1h, 1h30, 3h, 4h, 18h, 24h, after injection of oleic acid or physiological serum Brain total RNAs from mouse injected with acid oleic or physiological serum were profiled after hybridization with Agilent SurePrint G3 Mouse GE 8x60K Microarray to identify genes with differential expression

2015-01-01 | GSE52683 | GEO

Large scale transcriptomic characterization of mice lung inflammation during Acute Lung Injury (lung)

Project description:ARN sample of lung from mouse were obtened at 6 times points: 1h, 1h30, 3h, 4h, 18H, 24h, after injection of oleic acid or physiological serum Lung total RNAs from mouse injected with acid oleic or physiological serum were profiled after hybridization with Agilent SurePrint G3 Mouse GE 8x60K Microarray to identify genes with differential expression

2015-01-01 | GSE52682 | GEO

gene expression of Fibroblasts after hypoxia

Project description:to detect the gene expression of Fibroblasts after hypoxia, we used Agilent SurePrint G3 Human Gene Expression v3 for the transcriptional sequencing of fibroblasts with and without hypoxia to detect differentially expressed genes (DEGs) in hypoxia.

2022-01-25 | GSE194296 | GEO

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