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High-throughput sequencing of mouse liver transcriptome in Clock mutant animals

ABSTRACT: Circadian profile of polyA RNA by RNA-Seq, collected from ClockΔ19 mouse liver at CT22, CT28, CT34, CT40. RNA from three livers pooled per time point.

ORGANISM(S): Mus musculus

PROVIDER: GSE41082 | GEO | 2015/08/05

SECONDARY ACCESSION(S): PRJNA175697

REPOSITORIES: GEO

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High-throughput sequencing of mouse liver transcriptome in Clock mutant animals

Project description:Circadian profile of polyA RNA by RNA-Seq, collected from Clock?19 mouse liver at CT22, CT28, CT34, CT40. RNA from three livers pooled per time point. 4 Clock mutant samples with no replicates

2015-08-05 | E-GEOD-41082 | biostudies-arrayexpress

High-throughput sequencing of mouse liver transcriptome.

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High-throughput sequencing of mouse liver transcriptome.

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Clock rescue in the brain restores 12- and 24- hour rhythms in the liver

Project description:Circadian profiling was performed in mouse livers from three different genotypes/treatments: Wildtype, Clock mutant, and brain-specific Clock rescue. Rescuing Clock specifically in the brain partially rescued robust circadian and harmonic rhythms. Samples were collected every 2 hours for 48 hours from 2-4 mice per time point; samples were pooled and analyzed using Affymetrix Mouse Exon arrays, analyzed at the gene level.

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Effect of APAP-induced acute liver injury on WT and Nrf2KO zebrafish liver gene expression following 12 and 24 hours [RNA-Seq]

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Liver Clock Rescue

Project description:Circadian clocks drive 24-h rhythms of physiology and behavior. The circadian clock of hepatocytes has been shown to regulate glucose metabolism, and we were interested if rescuing liver clock function can reverse metabolic impairments in hyperphagic/obese Clock-D19 mutant mice. We compared transcripomte regulation in livers (at Zeitgeber time ZT10) of wild-type (C57BL/6J) and Clock-D19 mice and Clock-D19 mice with genetic rescue of liver clock function using hydrodynamic tail vein injection of a WT-CLOCK expression plasmid

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