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Human primary airway epithelial cell cultures infected with human- and swine-origin influenza viruses

ABSTRACT: Cultures of primary human airway epithelial cells (HAE cells) were exposed to an MDCK equivalent MOI of 0.01 of several swine- and human-origin influenza viruses and RNA was extracted at the 12, 16, and 24 hours post infection.

ORGANISM(S): Homo sapiens

PROVIDER: GSE41475 | GEO | 2012/10/11

SECONDARY ACCESSION(S): PRJNA177121

REPOSITORIES: GEO

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Human primary airway epithelial cell cultures infected with human- and swine-origin influenza viruses

Project description:Cultures of primary human airway epithelial cells (HAE cells) were exposed to an MDCK equivalent MOI of 0.01 of several swine- and human-origin influenza viruses and RNA was extracted at the 12, 16, and 24 hours post infection. 64 samples consiting of 5 influenza strains, 3 time points and 2 or 3 replicates at each time point. Expression of mock-infected samples measured at each time point.

2012-10-11 | E-GEOD-41475 | biostudies-arrayexpress

Host responses that correlate with respiratory droplet transmission of swine-origin influenza viruses in ferrets.

Project description:The determinants of influenza transmission remain poorly understood. Swine influenza viruses preferentially attach to receptors found in the upper airways; however, most swine influenza viruses fail to transmit efficiently from swine to humans, and from human-to-human. The pandemic 2009 H1N1 (H1N1pdm) virus was a rare exception of a swine virus that acquired efficient transmissibility from human-to-human, and is reflected in efficient respiratory droplet transmission in ferrets. We hypothesize that virus-induced host responses in the upper airways correlate with airborne transmission in ferrets. To address this question, we used the H1N1pdm virus and swine influenza A/swine/Hong Kong/201/2010 (HK201) virus that has comparable titre in the ferret nasopharynx, but it exhibits differential transmissibility in ferrets via respiratory droplet route. We performed a transcriptomic analysis of tissues from the upper and lower respiratory tract from ferrets infected with either H1N1pdm or HK201 viruses using ferret-specific Agilent oligonucleotide arrays. We found differences in the kinetics of the innate immune response elicited by these two viruses that varied across tissues.

2017-03-31 | GSE79282 | GEO

Influenza A Viruses of Human Origin in Swine, Brazil.

Project description: Not available

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Divergent Human-Origin Influenza Viruses Detected in Australian Swine Populations.

Project description: Not available

| S-EPMC6069171 | biostudies-literature

Human-Origin Influenza A(H3N2) Reassortant Viruses in Swine, Southeast Mexico.

Project description: Not available

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Secretome profiling of human airway epithelial cultures infected with respiratory syncytial virus

Project description:The respiratory epithelium is a polarised layer at the interface between the outside environment and deeper lung structures, overlaid by the epithelial lining fluid (ELF). This provides a mechanical and immunological barrier to inhaled particulates, such as viruses. Human respiratory syncytial virus (hRSV) is a major cause of disease in humans, and targets the respiratory epithelium. However, little is known of the disruption of the ELF proteome in the context of virus-driven respiratory illnesses. To address this, a proteomics approach was combined with an ex-vivo human airway epithelial model (HAE) to investigate the apical and basolateral secretome in hRSV-infected cultures. This demonstrated that several apically- and basolaterally-restricted proteins were subsequently secreted in both directions upon infection, while a number of proteins saw their apical/basolateral abundance ratios significantly altered. Furthermore, another 35 proteins were uniquely identified after hRSV treatment. Importantly, some of these changes were correlated in nasal aspirates (NA) from children with and without hRSV. This study showed that hRSV could affect airway secretions, and disrupted the directionality of the respiratory epithelium.

2022-03-01 | PXD004430 | Pride

Transcriptome analysis of human airway epithelium infected by Human Bocavirus 1

Project description:Human Bocavirus 1(HBoV1), which belongs to the genus Bocaparvovirus of the family Parvoviridae, infects well differentiated human airway epithelium which is at mitotically quiescent state. To systematicaly investigate the interaction between HBoV1 and primary human airway epithelium cultured at an air-liquid interface (HAE-ALI), RNA-seq was applied to study the transcriptome profile of HAE-ALI infected by HBoV1.

2017-10-22 | GSE102392 | GEO

Small RNA-seq analysis of human airway epithelium infected by Human Bocavirus 1

Project description:Human Bocavirus 1(HBoV1), which belongs to the genus Bocaparvovirus of the family Parvoviridae, infects well differentiated human airway epithelium which is at mitotically quiescent state. To systematicaly investigate the host and viral small RNA expression after HBoV1 infection of primary human airway epithelium cultured at an air-liquid interface (HAE-ALI), small RNA-seq was applied to study the small RNA transcriptome profile of HAE-ALI infected by HBoV1.

2019-01-16 | GSE123253 | GEO

Characterization of swine-origin H1N1 canine influenza viruses.

Project description: Not available

| S-EPMC7011970 | biostudies-literature

Gene expression data of human airway epithelium following human parechoviruses infection

Project description:Human parechoviruses (HPeVs), a poorly studied genus within the Picornaviridae family, are classified into 19 genotypes of which HPeV1 and HPeV3 are the most often detected. HPeVs pathogenesis is poorly understood as there are no animal models and the previous studies have only been conducted in immortalized monolayer cell cultures which do not adequately represent the characteristics of human tissues. To bridge this gap, we determined the polarity of infection, replication kinetics, and cell tropism of HPeV1 and HPeV3 in the well-differentiated human airway epithelial (HAE) model. We found the HAE cultures to be permissive for HPeVs. We speculated that differences in the airway epithelium host response may contribute to the distinct clinical outcomes and performed transcriptome analyses to compare the HAE gene expression profiles induced by HPeV1 and HPeV3 infection. Transcriptional profiling suggested that HPeV3 infection induced stronger immune activation than HPeV1. The polarized entry and genotype-specific host responses may contribute to the differences into the pathogenesis and clinical outcomes associated with HPeV1 and HPeV3.

2018-07-17 | GSE117183 | GEO

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