Genomics

Dataset Information

0

Mice Exposed in situ to Urban Air Pollution Exhibit Pulmonary Alterations in Lipid Droplet Synthesis Pathways


ABSTRACT: In the present study, we investigate pulmonary transcriptional responses in mice following exposure in situ to ambient air in a heavily polluted urban environment. Mature C57BL/CBA male mice were caged in sheds located in an urban area near two working steel mills and a major highway in Hamilton, Ontario, Canada. Control mice were housed in the same environment but received only high-efficiency particle-filtered air. Whole lung tissues were collected from mice exposed for 3 weeks, 10 weeks or for 10 weeks followed by 6 weeks in the laboratory (16 weeks total). DNA microarrays were used to explore changes in pulmonary gene expression in mice breathing ambient air versus HEPA-filtered air. Transcriptional profiling revealed changes in the expression of genes implicated in the lipid droplet synthesis pathway (plin, dgat2, lpl, s3-12, agpat2), antioxidants (ucp1). We postulate that exposure to particulate matter adsorbed with polycyclic aromatic hydrocarbons triggers lipid droplet synthesis (holding depots for lipids and malformed/excess proteins tagged for degradation) in the lungs, which act to sequester particulates adsorbed with toxic chemicals. Increased lipid droplet synthesis could potentially lead to endogenous/stressor-induced synthesis of reactive oxygen species and activation of antioxidant mechanisms. Further investigation into the stimulation of lipid droplet synthesis in the lung in response to air pollution is warranted in order to better understand these mechanistic changes and the resulting health implications.

ORGANISM(S): Mus musculus

PROVIDER: GSE41698 | GEO | 2013/09/30

SECONDARY ACCESSION(S): PRJNA178025

REPOSITORIES: GEO

Similar Datasets

| E-GEOD-41698 | biostudies-arrayexpress
| PRJNA178025 | ENA
2020-11-05 | GSE145840 | GEO
2010-04-25 | GSE15894 | GEO
2023-07-27 | GSE173545 | GEO
| E-GEOD-36810 | biostudies-arrayexpress
| E-GEOD-45166 | biostudies-arrayexpress
| E-GEOD-28082 | biostudies-arrayexpress
| E-GEOD-49963 | biostudies-arrayexpress
| PRJNA454321 | ENA