Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Influence of ATL-type Helios overexpression or WT-Helios/Ikaros knockdown on gene expression profile in Jurkat cells

ABSTRACT: It has been known that aberrant isoforms of Ikaros family ptoteins are expressed in malignant cells. We identified that PBMC of ATL (acute T-cell leukemia) patients expresses different patterns of Helios isoforms compared with normal PBMC. In order to investigate the biological impact of ATL-type Helios isoform, we conducted complehensive gene expression analyses in Jurkat cells overexpressing ATL-type Helios. Also, we investigated the influence of WT-Helios or WT-Ikaros knockdown on the gene expression profile and compared with that of the cells with ATL-type Helios overexpression.

ORGANISM(S): Homo sapiens

PROVIDER: GSE41796 | GEO | 2013/03/31

SECONDARY ACCESSION(S): PRJNA178211

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Influence of ATL-type Helios overexpression or WT-Helios/Ikaros knockdown on gene expression profile in Jurkat cells

Project description:It has been known that aberrant isoforms of Ikaros family ptoteins are expressed in malignant cells. We identified that PBMC of ATL (acute T-cell leukemia) patients expresses different patterns of Helios isoforms compared with normal PBMC. In order to investigate the biological impact of ATL-type Helios isoform, we conducted complehensive gene expression analyses in Jurkat cells overexpressing ATL-type Helios. Also, we investigated the influence of WT-Helios or WT-Ikaros knockdown on the gene expression profile and compared with that of the cells with ATL-type Helios overexpression. Total RNA samples from Jurkat cells were subjected to Cy-3 labeling followed by human whole genome gene expression microarray analyses.

2013-03-31 | E-GEOD-41796 | biostudies-arrayexpress

ChIP-seq IKAROS (Ikzf1) and HELIOS (Ikzf2)

Project description:Here we addressed the genome-wide DNA binding profile of Ikaros transcription factor in wild-type early hematopoietic progenitor cell line (HPC7). We also reported Ikaros and Helios DNA target sites in Helios KO or Ikaros KO HPC7 respectively, by performing Chromatin Immuno-Precipitation (ChIP) assay on genomic DNA.

2026-08-31 | GSE306060 | GEO

The Role of a Non-Canonical Splice Variant of the Helios Gene in the Differentiation of Jurkat T Lymphoblasts

Project description:In this study, the ectopic overexpressions of the wild-type Helios-1, non-canonical short isoforms Helios-Δ190-1186, Helios-Δ326-1431, and control in the Jurkat cells were utilized as the routine in vitro model of the T-cell acute lymphoblastic leukemia (T-ALL). We compared the microarray data for transcriptional analysis of the Jurkat cells with stable overexpression of full-length Helios-1, non-canonical short isoforms Helios-Δ190-1186, Helios-Δ326-1431, and mock transfected control.

2016-12-15 | GSE92416 | GEO

Single-cell transcriptomic analysis of Ikaros and/or Helios deficient HSPCs

Project description:Here we addressed the single cell transcriptome of hematopoietic stem and progenitor cells (HSPCs/LSK) purified from WT (Ikzf1+/+ Ikzf2+/+ Rosa26CreERT2/+), Helios KO (Ikzf2F/F Rosa26 CreERT2/+), Ikaros KO (Ikzf1F/F Rosa26 CreERT2/+) and DKO (Ikzf1F/F Ikzf2F/F Rosa26CreERT2/+) mice, 5 days after the last tamoxifen injection used to induce the deletion of Ikaros and/or Helios. We used single cell RNA sequencing (scRNA-seq) to determine the function of Ikaros and Helios in modulating the HSPCs lineage commitment and to study how their loss affect the composition of the HSPC compartment in terms of cell subtypes.

2026-08-31 | GSE306120 | GEO

Concerted action of Helios and Ikaros balances the strength of B cell receptor signaling and controls the expression of inositol 5-phosphatase SHIP

Project description:Inositol 5-phosphatase SHIP is differentially expressed in Ikaros and Helios deficient cells. In the absense of Ikaros SHIP is upregulated where as in the absense of Helios it is downregulated. Ikaros binds to the promoter of the SHIP gene. Article provides insight into the mechanisms of action Ikaros employs to regulate BCR signaling. Two replicates of Ikaros deficient and wild type DT40 avian B cells.

2010-07-19 | E-GEOD-20946 | biostudies-arrayexpress

Transcriptome analysis of Ikaros and/or Helios deficient LT-HSCs and MPPs

Project description:Here we addressed the mRNA transcriptome of Long-Term Hematopoietic Stem Cells (LT-HSCs) and Multipotent Progenitors (MPPs) purified from WT (Ikzf1+/+ Ikzf2+/+ Rosa26CreERT2/+), Helios KO (Ikzf2F/F Rosa26 CreERT2/+), Ikaros KO (Ikzf1F/F Rosa26 CreERT2/+) and DKO (Ikzf1F/F Ikzf2F/F Rosa26CreERT2/+) mice.

2026-08-31 | GSE306061 | GEO

Concerted action of Helios and Ikaros balances the strength of B cell receptor signaling and controls the expression of inositol 5-phosphatase SHIP

2010-07-19 | GSE20946 | GEO

Homo sapiens

Project description:Influence of ATL-type Helios overexpression or WT-Helios/Ikaros knockdown on gene expression profile in Jurkat cells

| PRJNA178211 | ENA

Redirecting the neo-substrate specificity of Cereblon-targeting PROTACs to Helios

Project description:Immunomodulatory imide drugs (IMiDs), such as thalidomide and its analogues, are some of the most commonly utilized E3 ligase ligands for the development of proteolysis targeting chimeras (PROTACs). While the canonical neo-substrates of IMiDs (i.e., Ikaros and Aiolos) are often considered to be unwanted off-targets of PROTACs, maintaining the degradation of these neo-substrates also provides the opportunity to synergistically degrade multiple proteins with a single compound. Here, we report the development of ALV-07-082-03, a CDK4/CDK6/Helios triple degrader that consists of palbociclib, an FDA-approved CDK4/6 inhibitor, conjugated to DKY709, a novel IMiD-based Helios degrader. Pharmacological co-degradation of CDK4/6 and Helios resulted in potent suppression of downstream signaling and proliferation in cancer cells, as well as enhanced de-repression of IL-2 secretion. Thus, not only do we demonstrate the possibility of rationally re-directing the neo-substrate specificity of PROTACs by incorporating alternative molecular glue molecules as E3 ligase ligands, but our findings also suggest that co-targeting CDK4/6 and Helios may have synergistic effects.

2022-09-07 | PXD036170 | Pride

ChIP-seq IKAROS (Ikzf1) and HELIOS (Ikzf2)

Project description:ChIP-seq IKAROS (Ikzf1) and HELIOS (Ikzf2)

| PRJNA1309047 | ENA