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Kc167_Inter-slide_Variability_Test

ABSTRACT: A large batch of Drosophila Kc167 total RNA was prepared and was used for 3 homotypic hybridizations in order to evaluate the inter-slide variability using the CDMC_Drosophila_7k2 platform. See Neal et al. 2003 for results and discussion. Keywords = Kc167 Keywords = Drosophila Keywords = Platform Variability Keywords: other

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE418 | GEO | 2003/05/27

SECONDARY ACCESSION(S): PRJNA84975

REPOSITORIES: GEO

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Kc167_Inter-slide_Variability_Test

Project description:A large batch of Drosophila Kc167 total RNA was prepared and was used for 3 homotypic hybridizations in order to evaluate the inter-slide variability using the CDMC_Drosophila_7k2 platform. See Neal et al. 2003 for results and discussion. Keywords = Kc167 Keywords = Drosophila Keywords = Platform Variability

2010-06-05 | E-GEOD-418 | biostudies-arrayexpress

Kc167_RNA_Quant_Eval

Project description:Three different amounts, 20 ug (GSM6155), 40 ug (GSM6157) and 80ug (GSM6158), of total Kc167 RNA were used in homotypic labelings to determine the optimal amount of RNA necesary to produce the most useable data using the CDMC_Drosophila_7k2 array. See Neal et al. 2003 for results and discussion. Keywords = Kc167 Keywords = Drosophila Keywords = RNA amount Keywords: other

2003-05-27 | GSE417 | GEO

SL2_vs_Kc167_Dif_Expr

Project description:The expression profiles of Drosophila Kc167 and SL2 cells were compared on the CDMC_Drosophila_7k2 array platform. Three independent RNA samples from each cell line were isolated and compared in a pairwise fashion. Dye-flip experiments were also performed. See Neal et al. 2003 for results and discussion. Keywords = Kc167 Keywords = SL2 Keywords = Drosophila Keywords: other

2003-05-27 | GSE419 | GEO

SL2_vs_Kc167_Dif_Expr

2010-06-05 | E-GEOD-419 | biostudies-arrayexpress

Detection of genes in different carbohydrates isolated by NSH using single channel slide based hybrdizations

Project description:Isolation of genes from Aspergillus nidulans grown in different complex carbohydrates by NSH and the NSH method was evaluated by using single channel non competitive slide based hybridizations Keywords = NSH Keywords = subtraction Keywords = aspergillus Keywords = polysaccharide Keywords = exopolygalacturonase Keywords: repeat sample

2004-03-14 | GSE783 | GEO

Parallel Hybridizations of six organs

Project description:Labeled organ cDNA samples from six organs were hybridized on one glass slide with identical conditions. The hybridizations were repeated for 19 times with 4 biological replications each with 5 technical replications. Keywords: repeat sample

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Transcriptional comparison of Drosophila cell lines using Kc167 as standard

Project description:A series of Drosophila melanogaster cell lines were each compared directly to the cell line Kc167. Keywords: Cell line comparison

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Time course of response of Kc cells to ecdysone

Project description:The changes in transcript titers are described for Drosophila Kc167 cells following 5-48 hr treatment with 20-hydroxyecdysone. Keywords: hormone response

2009-03-20 | GSE10926 | GEO

Deletions_TETs_WTs

Project description:Analysis of noncoding RNA processing phenotypes in mutant yeast strains, with isogenic wildtype strains as controls two-color microarrays, total RNA labeled directly with Alexa Ulysis 546 and 648 dyes, dye-swap, eight replicates of each oligo per slide, Lowess smoother, Hedge et al. 2002 protocols, see Peng, Robinson et al., 2003. Keywords: other

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Genomic maps of homotypic H2Av, heterotypic H2Av, input and 80mM salt soluble chromatin from Drosophila S2 cells

Project description:Nucleosomes that contain the histone variant H2A.Z are enriched around transcriptional start sites in many organisms. A single octameric nucleosome can contain two H2A.Z histones (homotypic) or one H2A.Z and one canonical H2A (heterotype). We generated high-resolution maps of homotypic and heterotypic Drosophila H2A.Z (H2Av) nucleosomes. Although homotypic and heterotypic H2Av nucleosomes map throughout most of the genome, homotypic nucleosomes are enriched and heterotypic nucleosomes are depleted downstream of active promoters and intron/exon boundaries. The distribution of homotypic H2A.Z nucleosomes resembles that of salt-soluble nucleosomes and shows evidence of displacement during transcriptional elongation. Homotypic nucleosomes are also depleted downstream of paused polymerases, where salt-soluble nucleosomes are conspicuously depleted. Our results suggest a model whereby H2A.Z enrichment patterns result from different structural interactions within the core of heterotypic and homotypic nucleosomes following disruption during transcriptional elongation. We analyzed two replicates for input, heterotypic and homotypic purifications. We sequenced one library for each of the single H2Av pulldown and 80mM salt soluble samples.

2010-11-01 | E-GEOD-21615 | biostudies-arrayexpress

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