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Gene expression profiles in juvenile hormone biosynthetic organ compared to other tissues in Bombyx mori

ABSTRACT: Juvenile hormone (JH) is produced and secreted by corpora allata (CA) during larval stages. JH biosynthesis from acetyl-CoA includes 13 enzymatic steps. Genes for 10 of those enzymes have been identified from Bombyx mori. It was also reported that they are basically expressed selectively in CA. Genes for the other 3 enzymes were recently reported from other insects, i.e., farnesyl diphosphate pyrophosphatase (FPPP) from Drosophila and farnesol dehydrogenase (FolD) and farnesal dehydrogenase (FalD) from Aedes. In order to reveal the expression profiles of these genes in Bombyx CA and to find other candidate genes involved in JH biosynthesis, we performed a transcriptomic analysis in corpora cardiaca-CA (CC-CA) complexes using a custom-made DNA microarray on which sequences from CC-CA EST libraries and genome-widely predicted genes are loaded. CC-CA complexes vigorously expressed JH-biosynthetic genes identified in Bombyx as well as genes involved in methyl-group metabolism and ribosomal protein genes; this is suitable for JH production. Bombyx counterparts of Drosophila FPPP and Aedes FolD genes, however, showed quite low level of expression. On the other hand, some other genes probably encoding oxidoreductases were expressed highly and selectively in CC-CA complex. Their products might function as FolD and/or FalD in Bombyx.

ORGANISM(S): Bombyx mori

PROVIDER: GSE41805 | GEO | 2014/12/31

SECONDARY ACCESSION(S): PRJNA178220

REPOSITORIES: GEO

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Project description:Microarray analyses identified 713 genes whose expression increased or decrease by 1.5-fold with a P value <0.01 in response to exposure to JH for 3-12 hr. 497 were up-regulated, and 228 were down-regulated. 12 genes showed differentially regulated pattern that depended on the time of exposure to JH. 73 of these genes (57 genes up-regulated and 17 genes down-regulated) showed the presence of DR4 element in their promoter regions. We also screened the promoter regions of genes whose products are distributed in different cellular locations and various functional groups and found that the DR4 element is present in 275 out of 2850 genes screened. The distribution of DR4 element containing genes varied depending on both cellular location and gene function. The genes whose products are localized to the nucleus or cytoplasm showed higher proportion of DR4 element containing genes when compared to the genes coding for products localized to the membrane. Genes belonging to immune response and ligand activated receptor functional groups showed higher number of genes that contained DR4 elements when compared to the genes belonging to protein kinase or transmembrane receptor functional groups. The DR4 elements identified in the promoter regions of D. melanogaster genes bound to the nuclear proteins isolated from JH III-treated Drosophila L57 cells. In addition, the expression of DR4 element containing genes identified from various functional groups was induced by JH III in Drosophila mbn2 cells grown in the medium containing 1 µM JH III. Presence of DR4 element in JH-responsive genes is conserved across species. This study also demonstrates the feasibility of using genome-wide analysis for identification of genes regulated by various hormones and other signaling molecules. Keywords: Time course We performed microarray analysis using RNA isolated form L57 cells that were exposed to 1 µM JH III for 0, 3, 6 and 12 hr and identified 497 genes that are induced by JH and 228 genes that are down-regulated by JH. Then, we searched the promoter regions of these genes and found that 57 out of these 497 JH induced genes, and 17 out of these 228 JH down-regulated genes had conserved DR4 element present in their promoters.

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Gene expression profiles in juvenile hormone biosynthetic organ compared to other tissues in Bombyx mori

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