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Comparison of Control and Tim-Fbl gene expression profiles

ABSTRACT: In order to identify genes affected in our model of PANK defficiency we profiled fly heads of female flies control or expressing fumble RNAi by the use of the tim-gal4 driver

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE42037 | GEO | 2015/01/12

SECONDARY ACCESSION(S): PRJNA178959

REPOSITORIES: GEO

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Comparison of Control and Tim-Fbl gene expression profiles

Project description:In order to identify genes affected in our model of PANK defficiency we profiled fly heads of female flies control or expressing fumble RNAi by the use of the tim-gal4 driver Female fly heads of the indicated strains were collected at 2 different circadian timepoints, RNA extracted, labeled and hybridized to Affymetrix oligonucleotide microarrays.

2015-01-12 | E-GEOD-42037 | biostudies-arrayexpress

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Project description:Control (+/cbtE1-UAS-cbt RNAi) or cabut RNAi flies (Tim-gal4, UAS-cbt RNAi) were starved for 16 hours and then exposed to food containing different concentrations of sucrose: 0, 25, 50 and 100 % for 18 hours. Fly heads were collected, RNA was extracted and RNA-seq libraries were prepared as previously described (Engreitz et al., 2013) For each sucrose concentration, two samples of cabut RNAi flies and one sample of control flies were sequenced.

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The E3 Ligase CUL3 physically interacts with TANGO10 protein in vivo

Project description:List of protein scores for putative TANGO10 interactors from mass spectrometry analysis of FLAG-tagged TANGO10 using either elav-GAL4 or tim-GAL4 at both ZT10 and ZT 22, as determined using Proteome Discoverer software (ThermoFisher). Fly heads from GAL4 heterozygotes were used as controls. The hits from FLAG-tagged twenty-four (TYF) proteomics were also excluded from the list to increase TANGO10-specificity. Proteins listed are those present in at least one elav-GAL4 sample and at least one tim-GAL4 sample but no negative controls.

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Transcription profiling of Drosophila fly heads treated with CHX, dexamethasone vs. controls

Project description:CLK targets from fly heads using the TIM-GAL4; UAS-CLKGR line Experiment Overall Design: Fly heads were stimulated with vehicle + CHX or dexamethasone + CHX

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CLK targets from fly heads

Project description:CLK targets from fly heads using the TIM-GAL4; UAS-CLKGR line Keywords: CLK TARGETS

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