Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression data of differentiated human erythroid cells with or without Tranylcypromine (TC) treatment

ABSTRACT: We found that LSD1 inhibition by a monoamine oxidase inhibitor, tranylcypromine (TC), could enhance fetal gamma globin expression. Global effects of TC on erythroid expession were conducted by HG-U219 array strips.

ORGANISM(S): Homo sapiens

PROVIDER: GSE42078 | GEO | 2013/02/16

SECONDARY ACCESSION(S): PRJNA179085

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Expression data of differentiated human erythroid cells with or without Tranylcypromine (TC) treatment

Project description:We found that LSD1 inhibition by a monoamine oxidase inhibitor, tranylcypromine (TC), could enhance fetal gamma globin expression. Global effects of TC on erythroid expession were conducted by HG-U219 array strips. Primary human erythroid cells, which differentiated from CD34+ cells for 8 days, were harvested before or and after TC treatment (0.5 ÂµM, 1.5 ÂµM or 5 ÂµM) for the gene expression analysis.

2013-02-16 | E-GEOD-42078 | biostudies-arrayexpress

Tranylcypromine specificity for monoamine oxidase is limited by promiscuous protein labelling and lysosomal trapping

Project description:Monoamine oxidases MAOA and MAOB catalyze important cellular functions such as the deamination of neurotransmitters. Correspondingly, MAO inhibitors are used for the treatment of severe neuropsychiatric disorders such as depression. A commonly prescribed drug against refractory depression is tranylcypromine, however, the side effects are poorly understood. In order to decipher putative off-targets, we synthesized two tranylcypromine probes equipped with either an alkyne moiety or an alkyne-diazirine minimal photocrosslinker for in situ proteome profiling. Surprisingly, LC-MS/MS analysis revealed low enrichment of MAOA and relatively promiscuous labeling of proteins. Photoprobe labeling paired with fluorescent imaging studies revealed lysosomal trapping which could be largely reverted by the addition of lysosomotropic drugs.

2020-10-20 | PXD018580 | Pride

Differentiating 3T3-L1 adipocytes, introduced with siRNA against aof2 and rfk genes, or treated with tranylcypromine

Project description:Adipogenic differentiation and metabolic adaptation are initiated through transcriptional and epigenetic reprogramming. In particular, dynamic changes in histone modifications may play central roles in the rearrangement of gene expression patterns. LSD1 (KDM1) protein, encoded by aof2 gene, is a histone demethylase, which is involved in transcriptional regulation. Since the enzymatic activity of LSD1 is FAD (flavin adenine dinucleotide)-dependent, its effects on gene expression may be influenced by FAD availability. To address the importance of histone demethylation in adipogenic differentiation and function, we performed cDNA microarray in LSD1-deficient 3T3-L1 cells as well as in the cells treated with LSD1 inhibitor tranylcypromine (TC). FAD-synthesizing enyme, riboflavin kinase (RFK) -deficient cells were also subjected to the microarray analysis. 3T3-L1 preadipocytes were transfected with aof2- or rfk- specific siRNA or control siRNA (siGL3) . 24 hours later, cells were subjected to adipogenic induction. 24 hours later, cells were harvested for total RNA extraction. For the TC treatment, TC was added to the adipogenic induction medium.

2012-04-04 | E-GEOD-18598 | biostudies-arrayexpress

Differentiating 3T3-L1 adipocytes, introduced with siRNA against aof2 and rfk genes, or treated with tranylcypromine

2012-04-05 | GSE18598 | GEO

Alicyclobacillus sp. TC strain:TC

Project description:Alicyclobacillus sp. TC strain:TC Genome sequencing and assembly

| PRJNA562382 | ENA

Escovopsis sp. TC strain:TC

Project description:Escovopsis sp. TC strain:TC Genome sequencing and assembly

| PRJNA388866 | ENA

Expression data from isolated endothelial cells from glioblastoma and normal brain cultured on Matrigel

Project description:We compared gene expressions between CD31-positive cells from glioblastoma (TECs) and endothelial cells from normal brain (NECs) In this dataset, expression data obtained from Affymetrix Human Genome U219 Array (HG-U219) are presented.

2022-05-18 | GSE137901 | GEO

Expression data from isolated endothelial cells from glioblastoma and normal brain cultured as monolayer on gelatin

2022-05-18 | GSE137900 | GEO

Transcriptome of thyroid cancer (TC) cells after coculture with TC-induced macrophages

Project description:RNA sequencing data of TPC-1 cells (TC cell line) after co-culture with TC-induced macrophages. We used an in vitro co-culture model of a human TC cell line, TPC1 (RET/PTC rearrangement), and human primary monocytes.

2020-09-21 | GSE120391 | GEO

Enterococcus sp. E843-TC isolate:E843-TC

Project description:Enterococcus sp. E843-TC isolate:E843-TC Genome sequencing

| PRJNA755459 | ENA