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Defining Gene Signatures for Protective Immunity to Tuberculosis in the Chinese Cynomolgus Macaque Model

ABSTRACT: Gene expression profiles in PPD-stimulated and unstimulated peripherhal blood mononuclear cells isolated from chinese cynomolgus macaques before and after BCG vaccination and before and after M. tuberculosis challenge were compared in animals able to control TB infection and those that developed TB disease in order to identify potential correlates of protection and/or biomarkers of disease.

ORGANISM(S): Macaca mulatta Macaca fascicularis

PROVIDER: GSE42273 | GEO | 2012/11/15

SECONDARY ACCESSION(S): PRJNA179516

REPOSITORIES: GEO

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Defining Gene Signatures for Protective Immunity to Tuberculosis in the Chinese Cynomolgus Macaque Model

Project description:Gene expression profiles in PPD-stimulated and unstimulated peripherhal blood mononuclear cells isolated from chinese cynomolgus macaques before and after BCG vaccination and before and after M. tuberculosis challenge were compared in animals able to control TB infection and those that developed TB disease in order to identify potential correlates of protection and/or biomarkers of disease. 6 macaques received BCG vaccination prior to challenge and were able to control TB infection (vaccinated controllers). 3 unvaccinated macaques were also able to control TB infection after challenge (unvaccinated controllers) and 3 other unvaccinated macaques developed TB disease and reached humane endpoint criteria (unvaccinated progressors). PBMCs isolated at 8 and 18 weeks post BCG-vaccination from the vaccinated controllers and at 6 weeks post M.tb challenge and at 2 time-points when the animals were naive in all animals were stimulated with PPD. RNA was extracted from the cells and hybridised to and Agilent rhesus macaque GE microarray in a one-colour hybridisation. Gene expression post-vaccination and/or post-challenge was compared with expression before vaccination/challenge when the animals were naive.

2012-11-15 | E-GEOD-42273 | biostudies-arrayexpress

Defining Gene Signatures for Protective Immunity to Tuberculosis in the Rhesus Macaque Model

Project description:Gene expression profiles in PPD-stimulated and unstimulated peripheral blood mononuclear cells isolated from rhesus macaques before and after M. tuberculosis challenge were compared in animals able to control TB infection and those that developed TB disease in order to identify potential correlates of protection and/or biomarkers of disease.

2018-12-16 | E-MTAB-7558 | biostudies-arrayexpress

Atlas of Cynomolgus Macaque Hematopoiesis

Project description:Self-renewal and differentiation are inherent properties of hematopoietic stem cells that are necessary to support hematopoiesis. However, the underlying mechanisms, especially in human, remain unclear. Here, using the cynomolgus macaque as a surrogate model, we develop a new gating strategy to isolate with high purity transplantable cynomolgus HSCs and generated a single-cell transcriptomic map of cynomolgus HSCs and progenitor cells, that covers the gestational period not analyzed in human. We show that hematopoietic cells from the late-1st to early-3rd trimester fetal liver and late-2nd trimester and thereafter bone marrow has repopulating potential, closely mimicking humans. Unexpectedly however, we found unlike in human, cynomolgus HSCs express CD38 but not CD33, indicating that these cellular counterparts are molecularly distinct. Our transcriptomic analysis reveals the presence of a direct differentiation pathway from HSCs to megakaryocyte lineages, lineage-primed multipotent progenitors and also identified putative HSC surface markers. Taken together, our comprehensive dataset highlights not only the utility of cynomolgus monkeys as model systems to study hematopoiesis but also their potential for translational applications.

2024-04-26 | GSE262140 | GEO

cynomolgus macaque miRNAs

Project description:Characterization of miRNAs distribution in cynomolgus macaque genome using small RNA sequencing

| PRJEB32837 | ENA

Phased cynomolgus macaque assembly

Project description:High-quality phased assembly of the cynomolgus macaque genome

| PRJEB37906 | ENA

Cynomolgus macaque model of H7N9 influenza infection

Project description:TheÂ objectiveÂ of this study is to characterize the response to newly emerged, highly pathogenic H7N9 influenza virus isolated from human patients in 2013 in China. This study examines the pathogenesis of H7N9 influenza in cynomolgus macaques.Â The study compares lung lesions to adjacent right lower lobe lung tissue in animals necropsied at days 3 and 6 post-infection (n=4 animals/timepoint).Â 3-4 lesions from each animal were collected and equal amounts of pooled RNA from lesions from individual animals at each time point were used for microarray. 8 cynomolgus macaques were infected via oral, intraocular, intranasal, and intratracheal administration of a combined total of 7x10^6 TCID50. Lungs, lung lesions, and trachea samples were collected from serial sacrifices of 4 animals each at day 3 and day 6. Infection produced a moderate-severe, self-limiting respiratory infection, and was not lethal. We performed microarray analysis (using Agilent Rhesus arrays) on all lungs, lung lesions, and trachea collected for the study.

2014-07-22 | E-GEOD-48976 | biostudies-arrayexpress

cynomolgus macaque miRNAs

Project description:Distribution characterization of conserved miRNAs in cynomolgus macaque genome using small RNA sequencing and homology searching

| PRJEB36250 | ENA

cynomolgus macaque miRNAs

Project description:Distribution characterization of conserved miRNAs in cynomolgus macaque genome using small RNA sequencing and homology searching

| PRJEB36952 | ENA

mitochondrial 12S rRNA sequences Cynomolgus macaque

Project description:mitochondrial 12S rRNA sequences Cynomolgus macaque

| PRJEB49348 | ENA

Cynomolgus macaque model of H7N9 influenza infection

Project description:The objective of this study is to characterize the response to newly emerged, highly pathogenic H7N9 influenza virus isolated from human patients in 2013 in China. This study examines the pathogenesis of H7N9 influenza in cynomolgus macaques. The study compares lung lesions to adjacent right lower lobe lung tissue in animals necropsied at days 3 and 6 post-infection (n=4 animals/timepoint). 3-4 lesions from each animal were collected and equal amounts of pooled RNA from lesions from individual animals at each time point were used for microarray.

2014-07-22 | GSE48976 | GEO

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