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BSO-treated blastocysts vs CTL-treated blastocysts

ABSTRACT: In order to understand how in vitro culture affects embryonic quality, we analyzed survival and global gene expression in bovine blastocysts after exposure to increased oxidative stress conditions of IVC. A pro-oxidant agents that act intra-cellularly by inhibiting GSH synthesis (0.4 mM buthionine sulfoximine [BSO]) was added from days 3 to 7, and transcriptomic analysis was then performed in resulting blastocysts. Precisely, after in vitro maturation and fertilization, bovine zygotes were culture in vitro in normal condition, then at day 3, embryos were allocated into culture in control or supplemented with BSO (0.4 mM) until day 7. At this time, blastocysts were harvested and analyzed. Our hypothesis was that BSO treatment will affect blastocyst survival and gene expression associated with low embryo quality

ORGANISM(S): Bos taurus

PROVIDER: GSE42280 | GEO | 2012/11/14

SECONDARY ACCESSION(S): PRJNA179526

REPOSITORIES: GEO

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BSO-treated blastocysts vs CTL-treated blastocysts

Project description:In order to understand how in vitro culture affects embryonic quality, we analyzed survival and global gene expression in bovine blastocysts after exposure to increased oxidative stress conditions of IVC. A pro-oxidant agents that act intra-cellularly by inhibiting GSH synthesis (0.4 mM buthionine sulfoximine [BSO]) was added from days 3 to 7, and transcriptomic analysis was then performed in resulting blastocysts. Precisely, after in vitro maturation and fertilization, bovine zygotes were culture in vitro in normal condition, then at day 3, embryos were allocated into culture in control or supplemented with BSO (0.4 mM) until day 7. At this time, blastocysts were harvested and analyzed. Our hypothesis was that BSO treatment will affect blastocyst survival and gene expression associated with low embryo quality 4 replicates of 10 blast/rep was produced for BSO and control treatment. According to a dye swap design with 2 colors, 4 arrays were used to compare BSO (green) against CTL (red) and 4 arrays were used to compared BSO (red) againt CTL (green).

2012-11-14 | E-GEOD-42280 | biostudies-arrayexpress

AAPH-treated blastocysts vs CTL-treated blastocysts

Project description:In order to understand how in vitro culture affects embryonic quality, we analyzed survival and global gene expression in bovine blastocysts after exposure to increased oxidative stress conditions of IVC. A pro-oxidant agents that act extra-cellularly by promoting ROS production (0.01 mM 2,2'-azobis (2-amidinopropane) dihydrochloride [AAPH]) was added from days 3 to 7, and transcriptomic analysis was then performed in resulting blastocysts. Precisely, after in vitro maturation and fertilization, bovine zygotes were culture in vitro in normal condition, then at day 3, embryos were allocated into culture in control or supplemented with AAPH (0.01 mM) until day 7. At this time, blastocysts were harvested and analyzed. Our hypothesis was that AAPH treatment will affect blastocyst survival and gene expression associated with low embryo quality

2012-11-14 | GSE42279 | GEO

Proteomic shift in mouse embryonic fibroblasts Pfa1 during L-Buthionine-sulfoximine (BSO) induced ferroptosis

Project description:This report provides data on ferroptosis induced proteomic response in immortalized mouse embryonic fibroblasts which are 4-OH-TAM-inducible Gpx4−/− (referred to as Pfa1 cells). Pfa1 cells are a valuable, widespread and well characterized model for ferroptosis research. We used L-Buthionine-sulfoximine (BSO) to induce ferroptosis and collected samples at 0h, 24h and 48 h after treatment. Untreated cells were used as a control. Pfa1 cells (kind gift from Marcus Conrad, Munich) were cultured in RPMI-1640 media (2 g/L glucose, 10% FBS, 2 mM Gibco GlutaMAX Supplement, 1% pen/strep) at 37C with 5% CO2 in a humidified incubator. Cells (300k) were seeded on Corning 100 mm tissue-culture treated culture dishes and incubated overnight. On the next day Pfa1 cells were treated with or without 30 µM BSO.

2023-04-12 | PXD041415 | Pride

AAPH-treated blastocysts vs CTL-treated blastocysts

Project description:In order to understand how in vitro culture affects embryonic quality, we analyzed survival and global gene expression in bovine blastocysts after exposure to increased oxidative stress conditions of IVC. A pro-oxidant agents that act extra-cellularly by promoting ROS production (0.01 mM 2,2'-azobis (2-amidinopropane) dihydrochloride [AAPH]) was added from days 3 to 7, and transcriptomic analysis was then performed in resulting blastocysts. Precisely, after in vitro maturation and fertilization, bovine zygotes were culture in vitro in normal condition, then at day 3, embryos were allocated into culture in control or supplemented with AAPH (0.01 mM) until day 7. At this time, blastocysts were harvested and analyzed. Our hypothesis was that AAPH treatment will affect blastocyst survival and gene expression associated with low embryo quality 4 replicates of 10 blast/rep was produced for AAPH and control treatment. According to a dye swap design with 2 colors, 4 arrays were used to compare AAPH (green) against CTL (red) and 4 arrays were used to compared AAPH (red) againt CTL (green).

2012-11-14 | E-GEOD-42279 | biostudies-arrayexpress

Effect of LPS and glutathione depletion on gene expression profiling in mouse macrophage-like cells

Project description:By microarray gene expression profiling, we investigated whether glutathione (GSH) has a signaling role in the response of RAW 264.7 cells to lipopolysaccharide (LPS). GSH was depleted by prior incubation with 120 µM buthionine sulfoximine (BSO) for 24 h, then cells were treated with 10 ng/ml LPS for 2 or 6 h. DNA microarray analysis was performed in triplicate samples in control and GSH-depleted cells, in the presence or absence of LPS.

2016-03-19 | GSE79397 | GEO

Comparison of the DNA methylation of in vitro cultured bovine SAM-treated blastocysts and non-treated blastocysts

Project description:Epigenetics (DNA methylation) profiling of bovine in vitro cultured expanded blastocysts (EB) comparing control non-treated expanded blastocysts with SAM-treated expanded blastocysts. S-Adenosyl methionine (SAM) is the global methyl donor providing methyl

2016-02-02 | GSE74081 | GEO

Transcriptomic signature in bovine blastocysts resulting from oxidative stress exposure at the time of embryonic genome activation

Project description:This SuperSeries is composed of the following subset Series: GSE42279: AAPH-treated blastocysts vs CTL-treated blastocysts GSE42280: BSO-treated blastocysts vs CTL-treated blastocysts Refer to individual Series

2012-11-14 | E-GEOD-42281 | biostudies-arrayexpress

Comparison of the transcription (coding and lncRNAs) of in vitro cultured bovine SAM-treated blastocysts and non-treated blastocysts

Project description:Transcriptome (total RNA) profiling of bovine in vitro cultured expanded blastocysts (EB) comparing control non-treated expanded blastocysts with SAM-treated expanded blastocysts. S-Adenosyl methionine (SAM) is the global methyl donor providing methyl group for variety of biomolecules such as DNA , histone, RNA, lipids and etc. Two-condition experiment, bovine non-treated expanded blastocysts (pools of 10) vs bovine SAM-treated expanded blastocysts. Four biological replicates of each tissue were hybridized to four two-color arrays in a dye-balanced design.

2016-02-29 | E-GEOD-75430 | biostudies-arrayexpress

Comparison of the transcription (coding and lncRNAs) of in vitro cultured bovine SAM-treated blastocysts and non-treated blastocysts

2016-02-29 | GSE75430 | GEO

Transcriptome Analysis Of Bovine Expanded Blastocysts Produced In Vivo Vs In Vitro Sof Opu

Project description:Comparison of gene expression from expanded bovine blastocysts collected 7 days after fertilization and produced in vivo vs in vitro-SOF-OPU Two kinds of 7 days post fertilization bovine embryos, in-vivo 7 days blastocysts vs. In vitro 7 days blastocysts, Biological replicates: 4 in-vitro, 4 in-vivo, protocol,extract and semen shared. Dye swap.

2012-03-31 | E-GEOD-35025 | biostudies-arrayexpress

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