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Agilent data from Arabidopsis thaliana enriched guard cell tissue of ost1-2,slac 1-5, gl1-1 and landsberg erecta

ABSTRACT: To identify genes of the guard cell transcriptome of Arabidopsis thaliana enriched guard cell samples of ost1-2 and slac1-5 mutants were compared to their wildtype.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE42470 | GEO | 2013/11/20

SECONDARY ACCESSION(S): PRJNA182965

REPOSITORIES: GEO

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Agilent data from Arabidopsis thaliana enriched guard cell tissue and leafs.

2013-06-04 | GSE38639 | GEO

Agilent data from Arabidopsis thaliana enriched guard cell tissue and leafs.

Project description:To identify genes of the guard cell transkriptome of Arabidopsis thaliana enriched guard cell samples were compared with total leaf tissue. Genes of the abscisic acid and humidity response of Arabidopsis thaliana guard cells were identified by treatment with ABA-Spray and low humidity. Ost1-2 and slac1-3 mutants were compared to their wildtype. total samples analysed are 35: 4 biolocigal independent replicates of: total leaf (COL-0) vs. enriched guard cells (COL-0); ABA-sprayed enriched guard cells (gl1-1) vs. control-sprayed enriched guard cells (gl1-1); enriched guard cells (slac1-3) vs. enriched guard cells (gl1-1);guard cells (ost1-2) vs. guard cells (ler);low humidity(20%rh) treated enriched guard cells (COL-0) vs. high humidity(80%) treated enriched guard cells (COL0)

2013-06-04 | E-GEOD-38639 | biostudies-arrayexpress

Agilent data from Arabidopsis thaliana enriched guard cell tissue and leafs.

2013-01-14 | GSE41054 | GEO

Agilent data from Arabidopsis thaliana enriched guard cell tissue of ost1-2,slac 1-5, gl1-1 and landsberg erecta

Project description:To identify genes of the guard cell transcriptome of Arabidopsis thaliana enriched guard cell samples of ost1-2 and slac1-5 mutants were compared to their wildtype. Total samples analysed are 11: biological independent replicates of: enriched guard cells (slac1-5) vs. enriched guard cells (gl1-1); enriched guard cells (ost1-2) vs. guard cells (ler);

2013-11-20 | E-GEOD-42470 | biostudies-arrayexpress

Agilent data from Arabidopsis thaliana enriched guard cell tissue and leafs.

Project description:To identify genes of the guard cell transcriptome of Arabidopsis thaliana enriched guard cell samples were compared with total leaf tissue. Genes of the abscisic acid and humidity response of Arabidopsis thaliana guard cells were identified by treatment with ABA-Spray and low humidity. total samples analysed are 24: 4 biological independent replicates of: total leaf (COL-0) vs. enriched guard cells (COL-0); ABA-sprayed enriched guard cells (gl1-1) vs. control-sprayed enriched guard cells (gl1-1); low humidity (20%rh) treated enriched guard cells (COL-0) vs. high humidity (80%) treated enriched guard cells (COL-0)

2013-01-14 | E-GEOD-41054 | biostudies-arrayexpress

Agilent data from Arabidopsis thaliana enriched guard cell tissues (GC) treated with high CO2 and darkness

Project description:Genes of the of Arabidopsis thaliana guard cells transcriptome that respond to high CO2 and darkness were identified and compared to the ABA- and low humidity treated samples of Experiment GSE41054 in Arabidopsis thaliana enriched guard cell samples.

2019-07-01 | GSE118520 | GEO

Whole genome transcriptome profiling of Arabidopsis thaliana guard cells under saline growth conditions

Project description:The overall objective was to document transcriptomic changes in the guard cells of Arabidopsis thaliana under short (1x) - and long-term (3x) saline growth conditions. Guard cells of Arabidopsis responded also to the intensity of the salt management by the microarray datasets clearly clustering in (-) salt, 1x salt and 3x salt. Similarly, more GO terms were significantly enriched in differentially expressed guard cell genes of 1 x salt than 3x salt treated plants.

2021-03-20 | GSE169230 | GEO

Sucrose modulation of the Arabidopsis thaliana guard-cell transcriptome

2012-11-26 | GSE37408 | GEO

Sucrose modulation of the Arabidopsis thaliana guard-cell transcriptome

Project description:Microarrays were used to evaluate the effect of sucrose on gene expression in guard cells. Strips of Arabidopsis leaves were incubated with sucrose or mannitol or no sugars, then the leaves were freeze dried and guard cells were dissected from the leaf strips and analyzed. RNA was extracted from guard cells dissected from leaf strips that had been treated with sucrose or with mannitol or no sugars as controls. Triplicate biological replicates were prepared for the treatments and controls. The RNA was amplified twice with T7 RNA polymerase and hybridized to Affymetrix ATH1 arrays.

2012-11-26 | E-GEOD-37408 | biostudies-arrayexpress

Microarray analysis of scap1 mutant and wild type in Arabidopsis thaliana guard cell protoplast

Project description:Stomata are highly specialized organs which consist of pairs of guard cells and regulate gas and water vapor exchange in plants. While early stages of guard cell differentiation have been described and were interpreted in analogy to processes of cell type differentiation in animals, the downstream development of functional stomatal guard cells remains poorly understood. We have isolated an Arabidopsis mutant, scap1 (stomatal carpenter 1), that develops irregularly shaped guard cells and lacks the ability to control stomatal aperture, including CO2-induced stomatal closing and light-induced stomatal opening. SCAP1 was identified as a plant-specific Dof-type transcription factor expressed in maturing guard cells but not in guard mother cells. SCAP1 regulates the expression of genes encoding key elements of stomatal functioning and morphogenesis, such as a K+ channel protein, MYB60 transcription factor, and pectin methyl esterase. Consequently, ion homeostasis was disturbed in scap1 guard cells, and esterification of extracellular pectins was impaired so that the cell walls lining the pores did not mature normally. We conclude that SCAP1 regulates essential processes of stomatal guard cell maturation and functions as a key transcription factor regulating the final stages of guard cell differentiation.

2013-02-28 | GSE43964 | GEO

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