Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Effects of ploidy and sex-locus genotype on gene expression patterns in the fire ant Solenopsis invicta [pupa]

ABSTRACT: In Hymenoptera, sex is determined by a single-locus complementary sex determining system (sl-CSD). Males are normally haploid (hemizygous at the sl-CSD locus) but if the genotype of the sl-CSD locus is homozygous, they develop into diploid males. Here, we study the effects of ploidy and the sl-CSD-locus genotype by comparing gene expression differences between haploid males, diploid males and virgin queens at three developmental stages, pupae, 1 day and 11 days after eclosion. Keywords: diploid males, sl-CSD, ploidy-spcific gene, sex-specific gene, doublesex, sex-biased gene

ORGANISM(S): Solenopsis invicta

PROVIDER: GSE42494 | GEO | 2012/12/06

SECONDARY ACCESSION(S): PRJNA183132

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Effects of ploidy and sex-locus genotype on gene expression patterns in the fire ant Solenopsis invicta [11d]

2012-12-06 | GSE42785 | GEO

Effects of ploidy and sex-locus genotype on gene expression patterns in the fire ant Solenopsis invicta [1d]

2012-12-06 | GSE42779 | GEO

Effects of ploidy and sex-locus genotype on gene expression patterns in the fire ant Solenopsis invicta [pupa]

Project description:In Hymenoptera, sex is determined by a single-locus complementary sex determining system (sl-CSD). Males are normally haploid (hemizygous at the sl-CSD locus) but if the genotype of the sl-CSD locus is homozygous, they develop into diploid males. Here, we study the effects of ploidy and the sl-CSD-locus genotype by comparing gene expression differences between haploid males, diploid males and virgin queens at three developmental stages, pupae, 1 day and 11 days after eclosion. Keywords: diploid males, sl-CSD, ploidy-spcific gene, sex-specific gene, doublesex, sex-biased gene Six-condition experiment: haploid male pupae, diploid male pupae, queen pupae, 1-day-old haploid male adults, 1-day-old diploid male adults, 1-day-old queen adults (accession number GSM1031732 - GSM1031734, GSM1031737 - GSM1031738, GSM1031740 - GSM1031742, GSM1031745 - GSM1031746; reprocessed for this study), 11-day-old haploid male adults, 11-day-old diploid male adults, 11-day-old queen adults (accession number GSM1031748 - GSM1031750, GSM1031753 - GSM1031754, GSM1031756 - GSM1031758, GSM1031761 - GSM1031762; reprocessed for this study). Biological replicates: 5 colonies (x 2 genotypes for each colony). Samples were labeled with Cy3 and were compared to the same common reference RNA labeled with Cy5.

2012-12-06 | E-GEOD-42494 | biostudies-arrayexpress

Effects of ploidy and sex-locus genotype on gene expression patterns in the fire ant Solenopsis invicta [11d]

2012-12-06 | E-GEOD-42785 | biostudies-arrayexpress

Effects of ploidy and sex-locus genotype on gene expression patterns in the fire ant Solenopsis invicta [1d]

2012-12-06 | E-GEOD-42779 | biostudies-arrayexpress

Nasonia Tiling Microarray Experiments for Expression Profiling Sexual Development (Pupa)

Project description:A key feature of Nasonia are their form of sex determination, called haplodiploidy. Females are diploid and develop from fertilized eggs, whereas males are haploid and develop parthenogenetically from unfertilized eggs. This form of sex determination is exploited by this experiment, by knowing the sex of the organisms from the earliest stages of development, so to trace the transcriptomes of sexual development of an insect. We conducted three replicates each using RNA from independent biological extractions of male and female early embryo (0-10 hrs), late embryo (18-30 hrs), 1st instar larvae, and pupae. Additional experiments were performed comparing transcription in adult males, adult females, testis and the female reproductive tract.

2018-04-15 | GSE46081 | GEO

Ploidy influences the cellular responses of gross chromosomal rearrangements in Saccharomyces cerevisiae

Project description:We investigated the effects of the ploidy on cellular response in strains carrying various types of gross chromosomal rearrangements. Fourteen mutated strains (6 haploid strains and 8 diploid strains) were compared to their associated parental strain (haploid or diploid parental strain). For each comparison, 2 microarray experiments implying biological replicates were performed.

2011-06-17 | E-GEOD-28285 | biostudies-arrayexpress

Scaling of cellular processes with ploidy

Project description:While most eukaryotic cells are diploid, with two chromosome sets, variances in ploidy are common. Despite the relative prevalence of ploidy changes and their relevance for pathology and evolution, a complete picture of consequences of altered ploidy is missing. We analyzed transcriptome and proteome changes in budding yeast Saccharomyces cerevisiae from haploid to tetraploid and found that the mRNA and protein abundance increases linearly with ploidy, but does not double with doubling the DNA content. Besides this linear increase, we found that pathways related to mitochondria and to cytoplasmic ribosomes and translation are differentially regulated. Indeed, with increasing ploidy the cells reduce mitochondrial content and this effect can be rescued by antioxidants. Moreover, cells of higher ploidy reduce their ribosome content while maintaining constant translational output. We show that this is an active process regulated via the Tor1 and Sch9 kinases and a transcriptional corepressor of rDNA transcription, Tup1. Similarly, human tetraploid cells downregulate their ribosome content via Tle1, a Tup1 homolog, demonstrating that the proteome remodeling is a conserved response to increased ploidy.

2022-08-15 | PXD022605 | Pride

Nasonia Tiling Microarray Experiments for Expression Profiling Sexual Development (Larva)

2018-04-15 | GSE46080 | GEO

Nasonia Tiling Microarray Experiments for Expression Profiling Sexual Development (Embryo18)

2018-04-15 | GSE46079 | GEO