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Comparison between V. cholerae MO10 delta PTS mutant and wild type strain grown as planktonic or surface-associated cells

ABSTRACT: The PTS system is a central regulatory cascade in bacteria. Here, Vibrio cholerae PTS role was investagated during biofilm formation

ORGANISM(S): Vibrio cholerae Vibrio cholerae MO10

PROVIDER: GSE42674 | GEO | 2012/12/10

SECONDARY ACCESSION(S): PRJNA182714

REPOSITORIES: GEO

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Comparison between V. cholerae MO10 delta PTS mutant and wild type strain grown as planktonic or surface-associated cells

Project description:The PTS system is a central regulatory cascade in bacteria. Here, Vibrio cholerae PTS role was investagated during biofilm formation Analysis used wild type MO10 cells as control samples for comparison to the delta PTS strain. Strains were grown as planktonic (sample 1) or surface attached cells (sample 2). Experiment was done in triplicate with dye swap, which represent 6 independent microarray hybridizations.

2012-12-10 | E-GEOD-42674 | biostudies-arrayexpress

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GNPS Vibrio cholerae in planktonic and biofilm growth modes exposed to Ag nanoparticles

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Gene expression profile of Vibrio cholerae N16961 grown in various conditions of culture

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Outer membrane vesicles and the outer membrane protein OmpU govern Vibrio cholerae biofilm matrix assembly

Project description:Biofilms are matrix-encased microbial communities that increase the environmental fitness and infectivity of many human pathogens including Vibrio cholerae. Biofilm matrix assembly is essential for biofilm formation and function. Known components of the V. cholerae biofilm matrix are the polysaccharide VPS, matrix proteins RbmA, RbmC, Bap1, and extracellular DNA, but the majority of the protein composition is uncharacterized. This study comprehensively analyzed the biofilm matrix proteome and revealed the presence of outer membrane proteins (OMPs). Outer membrane vesicles (OMVs) were also present in the V. cholerae biofilm matrix and were associated with OMPs and many biofilm matrix proteins suggesting that they participate in biofilm matrix assembly. Consistent with this, OMVs had the capability to alter biofilm structural properties depending on their composition. OmpU was the most prevalent OMP in the matrix, and its absence altered biofilm architecture by increasing VPS production. Using single-cell force spectroscopy, we further showed that OmpU, the matrix proteins RbmA, RbmC, and Bap1, and VPS contribute to cell-surface adhesion forces, which are critical for biofilm formation. Our findings provide new insights into the molecular mechanisms underlying biofilm matrix assembly in V. cholerae, which may open up new opportunities to develop inhibitors that specifically alter biofilm matrix properties and, thus, affect either the environmental survival or pathogenesis of Vibrio cholerae.

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