Project description:RNAseq experiments to identify gene expression changes following gamma irradiation Adult male Schistosoma mansoni (strain: NMRI) were subjected to 200 Gy of gamma irradiation and cultured in vitro for 48hr hours. After 48hr germ line tissues were removed and RNA was extracted from remaining somatic tissues. Samples were processed for RNAseq using Illumina procedures.
Project description:Schistosomes are parasitic flatworms that cause one of the most prevalent infectious diseases from which millions of people are currently suffering. Here, we construct a cell type atlas of juvenile schistosomes to capture germline stem cells (GSCs) in the course of de novo gonadal development.