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To detect CENP-A enrichment on Chromosome 1,5 &7 in C. albicans centromere deleted clones

ABSTRACT: ChIP Seq revealed presence of unique CEN-proximal neocentromere peaks in CEN deleted clones. Using 10 million reads generated from ChIP-seq for each sample, chromosome wide maps for CENP-A enrichment were generated .

ORGANISM(S): Candida albicans

PROVIDER: GSE42907 | GEO | 2012/12/21

SECONDARY ACCESSION(S): PRJNA183852

REPOSITORIES: GEO

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To detect CENP-A enrichment on Chromosome 1,5 &7 in C. albicans centromere deleted clones

Project description:ChIP Seq revealed presence of unique CEN-proximal neocentromere peaks in CEN deleted clones. Using 10 million reads generated from ChIP-seq for each sample, chromosome wide maps for CENP-A enrichment were generated . Examination of CENP-A enrichment in Input/IP samples of various CEN deleted C. albicans clones

2012-12-21 | E-GEOD-42907 | biostudies-arrayexpress

Genome-wide maps of CENP-A nucleosomes in three neocentromere-containing cell lines

Project description:We used native ChIP-seq of CENP-A-containing particles from normal centromeres on alpha-satellite DNA and three naturally-occurring neocentromeres to test the proposed models for the major form of the fundamental repeating unit of centromeric chromatin. We found that the predominant form of the CENP-A particle at the centromere is an octameric nucleosome with loose terminal DNA. Additionally, we found CENP-A nucleosomes are strongly phased on the 171 bp alpha-satellite monomers of normal centromeres, and also display strong positioning and neocentromeres. Comparison of CENP-A and bulk nucleosome DNA lengths and positions in three different human neocentromere-containing cell lines

2013-05-04 | E-GEOD-44724 | biostudies-arrayexpress

Phosphorylation of CENP-A on serine 7 does not control centromere function

Project description:CENP-A is the histone H3 variant necessary to specify the location of all eukaryoticcentromeres via its CENP-A targeting domain and either one of its terminal regions. In humans, several post-translational modifications occur on CENP-A, but their role in centromere function remains controversial. One of these modifications of CENP-A, phosphorylation on serine 7,has been proposed to control centromere assembly and function. Here, using gene targeting at both endogenous CENP-A alleles and gene replacement in human cells, we demonstrate that a CENP-A variant that cannot be phosphorylated at serine 7 maintains correct CENP-C recruitment, faithful chromosome segregation and long-term cell viability. Thus, we conclude that phosphorylation of CENP-A on serine 7 is dispensable to maintain correct centromere dynamics and function.

2019-01-28 | PXD012163 | Pride

Genome-wide maps of chromatin state (heterochromatin H3K9me2 or centromeric histone H3 variant CENP-A/Cnp1) in heterozygous deletion diploid mhf2∆/+ and the meiotic haploid progeny of heterozygous deletion diploid wip1∆/+, mhf1∆/+ and mhf2∆/+.

Project description:We report the high-throughput profiling of histone modification (H3K9me2) or histone variant CNEP-A/Cnp1 in fission yeast Schizosaccharomyces pombe. By obtaining 1-10 ng immunoprecipitated DNA, we generated genome-wide H3K9me2 or CENP-A/Cnp1 maps of heterozygous deletion diploid mhf2∆/+ and the meiotic haploid progeny of heterozygous deletion diploid wip1∆/+, mhf1∆/+ and mhf2∆/+ in fission yeast. We find that centromere inactivation and neocentromere formation occur independently and postzygotically in single depletion of CENP-T-W-X-S.

2019-09-28 | GSE117954 | GEO

DNA replication-mediated error correction of ectopic CENP-A deposition maintains centromere identity

Project description:Chromatin assembled with histone H3 variant CENP-A is the heritable epigenetic determinant of human centromere identity. Using genome-wide mapping and reference models for 23 human centromeres, CENP-A is shown in early G1 to be assembled into nucleosomes within megabase, repetitive a-satellite DNAs at each centromere and onto 11,390 sites on the chromosome arms. Centromere-bound CENP-A is found to be quantitatively maintained during DNA replication by coordinated action of the MCM2 helicase, CAF1, HJURP, and the CCAN network of constitutive centromere components. CCAN serves to tether CENP-A removed by MCM2, thereby enabling local reassembly onto both daughter centromeres with identical DNA sequence preferences and nucleosome phasing as the loading in G1 and independent of CENP-B. Conversely, without CCAN-mediated tethering, DNA replication removes CENP-A from sites on the chromosome arms. Our data identify an MCM2/CAF1/HJURP- and CCAN-dependent error correction mechanism that acts in S-phase to maintain CENP-A-dependent centromere identity.

2019-03-20 | GSE111381 | GEO

Swr1 mediated H2A.ZPht1 incorporation designates centromere DNA for de novo CENP-ACnp1 assembly

Project description:The centromere is the location on each chromosome that directs the assembly of the kinetochore. The underlying hallmark of centromeres in most eukaryotes is the presence of specialised nucleosomes in which canonical histone H3 is replaced by the histone H3 variant CENP-A. The molecular events that mediate a programme to install CENP-A in place of histone H3, at centromeres and how the centromeric chromatin is reorganised for CENP-A assembly during cell cycle remain poorly characterised. Histone H2A variant, H2A.Z is linked to transcriptional competence, in maintaining heterochromatin silencing and enriched in CENP-A chromatin. Our analyses demonstrate that H2A.ZPht1 and the Swr1 complex are associated with CENP-ACnp1 chromatin in fission yeast. Swr1 and Msc1 regulate H2A.ZPht1 deposition at centromeres and along with H2A.ZPht1 maintain CENP-ACnp1 chromatin integrity. Additionally, they coordinate the deposition of CENP-ACnp1 through the cell cycle, coupled with eviction of histone H3 from centromeres. Based on our results, we propose that the centromere is programmed to the widespread incorporation of H2A.ZPht1 via Swr1, and that H2A.ZPht1 dynamics likely play a role in regulating centromeric chromatin by influencing CENP-A incorporation.

2020-12-01 | GSE106585 | GEO

Centromere maintenance during DNA replication by retention of centromeric CENP-A coupled to removal of non-centromeric CENP-A

Project description:Mass spectrometry was used to determine the components which associate with CENP-ATAP during replication in late S phase, following affinity purification of CENP-A nucleosomes. CENP-ATAP was immunoprecipitated from the chromatin fraction of randomly cycling cells or late S synchronized cells. Mass spectrometry was performed on Velos Orbitrap.

2019-03-18 | MSV000083592 | MassIVE

Genome-wide maps of histone H3 variant CENP-A/Cnp1 in the meiotic haploid progeny of wip1∆/+, mhf1∆/+ and mhf2∆/+ and genome-wide maps of histone H3 variant CENP-A/Cnp1 in mhf2+ (or mhf2∆) carrying the inactivated Centromere 1 or Centromere 2.

Project description:We report the high-throughput profiling of histone variant CNEP-A/Cnp1 in fission yeast Schizosaccharomyces pombe. By obtaining 1-10 ng immunoprecipitated DNA, we generated genome-wide CENP-A/Cnp1 maps of the wild type meiotic haploid progeny of heterozygous deletion diploid wip1∆/+, mhf1∆/+ and mhf2∆/+ and CENP-A/Cnp1 maps of mhf2+ carrying the inactivated Centromere 1 or Centromere 2. We find that CENP-A/Cnp1 spreading into the pericentromeric regions in wild type meiotic progeny of heterozygous deletion diploid wip1∆/+, mhf1∆/+ and mhf2∆/+.

2019-09-28 | GSE131223 | GEO

Genome-wide maps of chromatin state (centromeric histone H3 variant CENP-A/Cnp1) in clr4∆ and the transformants from the deletion of clr4 in mhf2∆ (cen2_inactive) or mhf2+ (cen1_inactive).

Project description:We report the high-throughput profiling of histone variant CNEP-A/Cnp1 in fission yeast Schizosaccharomyces pombe. By obtaining 1-10 ng immunoprecipitated DNA, we generated genome-wide CENP-A/Cnp1 maps of clr4∆ and the transformants from the deletion of clr4 in mhf2∆ (cen2_inactive) or mhf2+ (cen1_inactive) in fission yeast. We find that heterochromatin is not required for the induction of centromere inactivation in mhf2∆ and the maintenance of neocentromere in mhf2+.

2019-09-28 | GSE131226 | GEO

Genome-wide maps of chromatin state (heterochromatin H3K9me2 or centromeric histone H3 variant CENP-A/Cnp1) in both mhf2∆ and mhf2+ carrying the inactivated Centromere 1 or Centromere 2.

Project description:We report the high-throughput profiling of histone modification (H3K9me2) or histone variant CNEP-A/Cnp1 in fission yeast Schizosaccharomyces pombe. By obtaining 1-10 ng immunoprecipitated DNA, we generated genome-wide H3K9me2 or CENP-A/Cnp1 maps of both mhf2∆ and mhf2+ carrying the inactivated Centromere 1 or Centromere 2 in fission yeast. We find that neocemtromeres are formed preferably at pericentromeric regions in single depletion of CENP-T-W-X-S.

2019-09-28 | GSE117955 | GEO

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