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Androgen arrays for Resveratrol-Induced Gene Expression Profiles in Human Prostate Cancer

ABSTRACT: Arrays for Expression levels of androgen-responsive genes in LNCaP cells exposed to dihydrotestosterone, R1881. Gene-expression changes in LNCaP treated with 1 nmol/L R1881 at 7, 9, 18, 24, 50, and 72 hours; 10 nmol/L dihydrotestosterone at 18 and 50 hours; 100 nmol/L dihydrotestosterone and 1 5mol/L dihydrotestosterone at 24 hours; and androgen deprivation at 46 and 70 hours. This set corresponds to Figure 2 of the publication. An all pairs experiment design type is where all labeled extracts are compared to every other labeled extract. Keywords: all_pairs

ORGANISM(S): Homo sapiens

PROVIDER: GSE4300 | GEO | 2006/02/25

SECONDARY ACCESSION(S): PRJNA104663

REPOSITORIES: GEO

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Androgen arrays for Resveratrol-Induced Gene Expression Profiles in Human Prostate Cancer

Project description:Arrays for Expression levels of androgen-responsive genes in LNCaP cells exposed to dihydrotestosterone, R1881. Gene-expression changes in LNCaP treated with 1 nmol/L R1881 at 7, 9, 18, 24, 50, and 72 hours; 10 nmol/L dihydrotestosterone at 18 and 50 hours; 100 nmol/L dihydrotestosterone and 1 5mol/L dihydrotestosterone at 24 hours; and androgen deprivation at 46 and 70 hours. This set corresponds to Figure 2 of the publication. An all pairs experiment design type is where all labeled extracts are compared to every other labeled extract. Computed

2006-02-24 | E-GEOD-4300 | biostudies-arrayexpress

Transcription profiling of human prostate cancer cells treated with resveratrol

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Androgen receptor-coregulators hormone responsiveness LNCaP / GSF

Project description:The text description is: LNCaP are androgen receptor expressing prostate carcinoma cells. Genital skin fibroblasts also express the androgen receptor. Cells were either proliferating or they were G0-arrested. Treatment of cells was performed with either the androgen DHT (dihydrotestosterone), the androgen analogue R1881 (methyltrienolone), or the solvent ethanol. Some hybridizations were performed in the type 1 design. In these cases, the hormone treated sample was hybridized against the ethanol treated sample on the same microarray. Hormone mediated induction or repression of gene transcription can directly be deduced from R/G normalized ratios on arrays. For other experiments, the ethanol treated control and the hormone treated experiment were hybridized on separate microarrays against a common reference of RNA. This reference was composed out of common reference batch CRG (50%) and a fibroblast RNA (50%). This reference was called mixed reference. For all experiments, the same batch of mixed reference was used. A compound treatment design type is where the response to administration of a compound or chemical (including biological compounds such as hormones) is assayed. Compound Based Treatment: Ethanol, R1881 (methyltrienolone), or DHT (dihydrotestosterone) Cell Line: LNCaP (prostate cancer) or foreskin fibroblasts Culture Synchrony: proliferation / Go-arrest Keywords: compound_treatment_design

2006-10-12 | GSE4454 | GEO

Androgen receptor-coregulators hormone responsiveness LNCaP / GSF

2010-07-01 | E-GEOD-4454 | biostudies-arrayexpress

Transcription profiling of human androgen receptor expressing prostate carcinoma cell line LNCaP and normal human foreskin fibroblasts expressing the androgen receptor treated with dihydrotestosterone (DHT), ethanol, or untreated vs. a common reference, see E-SMDB-3636

Project description:The text description is: LNCaP are androgen receptor expressing prostate carcinoma cells. Genital skin fibroblasts also express the androgen receptor. Cells were either proliferating or they were G0-arrested. Treatment of cells was performed with either the androgen DHT (dihydrotestosterone), the androgen analogue R1881 (methyltrienolone), or the solvent ethanol. Hybridizations were performed in the type 1 design for arrays # 1-8. In these cases, the hormone treated sample was hybridized against the ethanol treated sample on the same microarray. Hormone mediated induction or repression of gene transcription can directly be deduced from R/G normalized ratios on arrays. For other experiments (#9-24), the ethanol treated control and the hormone treated experiment were hybridized on separate microarrays against a common reference of RNA. This reference was composed out of common reference batch CRG (50%) and a fibroblast RNA (50%). This reference was called mixed reference. For all experiments, the same batch of mixed reference was used.

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Gene expression of LNCaP cells grown in 2D cultures and polyethlene glycol-based hydrogels with the presence of synthetic androgen, R1881

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Gene expression of LNCaP cells grown in polyethlene glycol-based hydrogels alone (monocultures) and grown with human osteoblasts as tissue engineered bone (co-culture) with the presence of synthetic androgen, R1881

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Prostate cancer cell line LNCaP treated for 2, 4, 6, 8 h with the synthetic androgen R1881 (1nM)

Project description:Time series of the prostate cancer cell line LNCaP, treated for 2, 4, 6 and 8 hours with the synthetic androgen R1881. As control, the cells were cultured for 2, 4, 6 and 8 hours in the presence of the same concentration of solvent (ethanol). With this short treatment time, we aimed to identify mainly direct targets of the androgen receptor. LNCaP has a very low growth rate in steroid stripped medium and resumes growth on addition of androgens. Keywords: Time course

2006-04-01 | GSE4027 | GEO

Hormone-Independence of Prostate Cancer Cells is Supported by the Androgen Receptor without Binding to Classical Response Elements

Project description:Treatment of late passage (LP50) LNCaP cells with R1881 (androgen) and AR shRNA identified a gene program controlled by androgen receptor in the absence of androgen. Gene expression in late passage (LP50) LNCaP cells that had enhanced androgen-independent growth was determined in androgen-depleted medium in response to R1881 or AR knock down via AR shRNA

2011-01-28 | E-GEOD-22483 | biostudies-arrayexpress

Hormone-Independence of Prostate Cancer Cells is Supported by the Androgen Receptor without Binding to Classical Response Elements

Project description:Treatment of late passage (LP50) LNCaP cells with R1881 (androgen) and AR shRNA identified a gene program controlled by androgen receptor in the absence of androgen.

2011-01-28 | GSE22483 | GEO

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