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Identification of GATA1 transcriptional regulation in the megakaryoblastic cell line Meg01

ABSTRACT: chIP-on-chip analysis of GATA1 in the megakaryoblastic cell line Meg01.

ORGANISM(S): Homo sapiens

PROVIDER: GSE43018 | GEO | 2013/07/01

SECONDARY ACCESSION(S): PRJNA184062

REPOSITORIES: GEO

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Identification of GATA1 transcriptional regulation in the megakaryoblastic cell line Meg01

Project description:chIP-on-chip analysis of GATA1 in the megakaryoblastic cell line Meg01. The IP was obtained with a GATA1 C-terminus antibody. The control sample was a normal goat IgG pull down done side by side with the GATA1 (C-20) antibody using the same soluble chromatin sample from non-treated Meg-01 cells. A 2-chip microarray set (Agilent 240K) was used for the chIP-on-chip analysis.

2013-07-01 | E-GEOD-43018 | biostudies-arrayexpress

Overexpression of GATA1 confers resistance to chemotherapy in acute megakaryocytic leukemia

Project description:Gene expression study of shRNA knockdown of GATA1 in the megakaryoblastic cell line Meg01.

2013-07-01 | GSE42879 | GEO

Overexpression of GATA1 confers resistance to chemotherapy in acute megakaryocytic leukemia

Project description:Gene expression study of shRNA knockdown of GATA1 in the megakaryoblastic cell line Meg01. Cell culture samples from two Meg01 clones (GATA1 4-14 and GATA1 5-13) were compared against a negative control infection sample (GATA1-neg). Two dye-swap microarrays were completed for each clone.

2013-07-01 | E-GEOD-42879 | biostudies-arrayexpress

Homo sapiens

Project description:Identification of GATA1 transcriptional regulation in the megakaryoblastic cell line Meg01

| PRJNA184062 | ENA

Identification of Acute megakaryoblastic leukemia (AMKL) deregulated genes by RNAseq

Project description:Acute megakaryoblastic leukemia (AMKL) is a heterogeneous disease generally associated with poor prognosis. Gene expression profiles indicate the existence of distinct molecular subgroups, and several genetic alterations have been characterized in the past years, including the t(1;22)(p13;q13) and the trisomy 21 associated with GATA1 mutations. However, the majority of patients do not present known mutations, and the limited access to primary patient leukemic cells impedes the efficient development of novel therapeutic strategies. In this study, using a xenotransplantation approach, we have modeled human pediatric AMKL in immunodeficient mice. Analysis of high-throughput RNA sequencing identified recurrent fusion genes defining new molecular subgroups.

2016-05-21 | E-MTAB-4697 | biostudies-arrayexpress

caArray_orkin-0038: Identification of distinct molecular phenotypes in acute megakaryoblastic leukemia by gene expression profiling

Project description:Individuals with Down syndrome (DS) are predisposed to develop acute megakaryoblastic leukemia (AMKL), characterized by expression of truncated GATA1 transcription factor protein (GATA1s) due to somatic mutation. The treatment outcome for DS-AMKL is more favorable than for AMKL in non-DS patients. To gain insight into gene expression differences in AMKL, we compared 24 DS and 39 non-DS AMKL samples. We found that non-DS-AMKL samples cluster in two groups, characterized by differences in expression of HOX/TALE family members. Both of these groups are distinct from DS-AMKL, independent of chromosome 21 gene expression. To explore alterations of the GATA1 transcriptome, we used cross-species comparison with genes regulated by GATA1 expression in murine erythroid precursors. Genes repressed after GATA1 induction in the murine system, most notably GATA-2, MYC, and KIT, show increased expression in DS-AMKL, suggesting that GATA1s fail to repress this class of genes. Only a subset of genes that are up-regulated upon GATA1 induction in the murine system show increased expression in DS-AMKL, including GATA1 and BACH1, a probable negative regulator of megakaryocytic differentiation located on chromosome 21. Surprisingly, expression of the chromosome 21 gene RUNX1, a known regulator of megakaryopoiesis, was not elevated in DS-AMKL. Our results identify relevant signatures for distinct AMKL entities and provide insight into gene expression changes associated with these related leukemias.

2016-06-17 | GSE83449 | GEO

Genome-wide maps of GATA1 occupany in induced G1E ER4 cell line.

Project description:we mapped the locations of DNA segments occupied by GATA1 using chromatin immunoprecipitation (ChIP). We have produced genome-wide GATA1 ChIP datasets after restoration and activation in G1E-ER4 cells. we employed the sequence census methodology of ChIP-seq , using Illumina GA2 technology to produce 23 million reads (36 nucleotides long) uniquely mapped to the mouse genome (mm8 assembly) for the GATA1 ChIP DNA and 15 million mapped reads for the input DNA

2009-09-19 | GSE18164 | GEO

Genome-wide maps of GATA1 occupany in induced G1E ER4 cell line.

2009-09-19 | E-GEOD-18164 | biostudies-arrayexpress

Consequences of GATA1 expression in Gata1- G1ME Murine Megakaryocyte/Erythrocyte Progenitor Cell Line

Project description:G1ME cells are GATA1-deficient murine bipotential megakaryocyte/erythrocyte progenitor cells derived from Gata1-negative murine ES cells. In order to assess the impact of GATA1 on gene regulation and cell differentiation, an expression construct was used to transiently produce high levels of GATA1. Cells transduced with this construct or a vector control were harvested at 18 and 42 hours, and gene expression was analyzed using Affymetrix MOE430 version 2 arrays.

2009-06-10 | GSE14980 | GEO

Consequences of GATA1 expression in Gata1- G1ME Murine Megakaryocyte/Erythrocyte Progenitor Cell Line

2009-06-10 | E-GEOD-14980 | biostudies-arrayexpress

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