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Caco-2 cells: cultured in conventional vs apical anaerobic conditions

ABSTRACT: Transcriptional profiling of Caco-2 cells comparing Caco-2 monolayers cultured in a custom built co-culture chamber, either inside a 5% CO₂ incubator (conventional cell culture environment) or an anaerobic workstation (apical anaerobic environment) for 12 hours.

ORGANISM(S): Homo sapiens

PROVIDER: GSE43049 | GEO | 2012/12/21

SECONDARY ACCESSION(S): PRJNA184193

REPOSITORIES: GEO

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Caco-2 cells: cultured in conventional vs apical anaerobic conditions

Project description:Transcriptional profiling of Caco-2 cells comparing Caco-2 monolayers cultured in a custom built co-culture chamber, either inside a 5% CO₂ incubator (conventional cell culture environment) or an anaerobic workstation (apical anaerobic environment) for 12 hours. Two-condition experiment with dye swap, A vs B, 6 biological replicates

2012-12-21 | E-GEOD-43049 | biostudies-arrayexpress

Gene expression in Caco-2 cells co-cultured with bacteria in an apical anaerobic environment

Project description:Transcriptional profiling of Caco-2 cells co-cultured with Faecalibacterium prausnitzii DSM17677, Lactobacilus rhamnosus HN001, UV-killed F. prausnitzii, or no bacteria in an apical anaerobic environment for four hours.

2013-08-19 | GSE49900 | GEO

Gene expression in Caco-2 cells co-cultured with bacteria in an apical anaerobic environment

2013-08-19 | GSE49900 | GEO

Gene expression in Caco-2 cells co-cultured with bacteria in an apical anaerobic environment

2013-08-19 | E-GEOD-49900 | biostudies-arrayexpress

Caco-2 cell gene expression following co-culture with Lactobacillus casei and Bifidobacterium breve

Project description:To characterize how symbiotic bacteria affect the lolecular and cellular mechanisms of epithelial homeostasis, human colonic Caco-2 cells were co-culture with Lactobacillus casei and Bifidobacterium breve.

2013-04-09 | GSE37369 | GEO

Caco-2 cells cultured in the presence of Lactobacillus fermentum strains AGR1485 or AGR1487

2013-05-01 | GSE46352 | GEO

Transcriptional response of S.cerevisiae to aeration after anaerobic growth

Project description:To determine the transcriptional changes that occur when yeast is shifted from anaerobic growth to an aerobic environment over a period of 120 minutes. Keywords: time course, stress response, environmental response, aerobic, anaerobic

2007-03-06 | GSE7140 | GEO

Caco-2 cell gene expression following co-culture with Lactobacillus casei and Bifidobacterium breve

2013-04-09 | E-GEOD-37369 | biostudies-arrayexpress

Caco-2 cells cultured in the presence of Lactobacillus fermentum strains AGR1485 or AGR1487

Project description:Transcriptional profiling of Caco-2 cells co-cultured with L. fermentum AGR1487 (isolated from IBD patient), Caco-2 cells co-cultured with L. fermentum AGR1485 (isolated from healthy volunteer), or Caco-2 cells alone (Control). Three-condition experiment with dye swap, A vs B, A vs C, B vs C, 8 biological replicates: A = Control, B = AGR1487, C = AGR1485

2013-05-01 | E-GEOD-46352 | biostudies-arrayexpress

Anaerobic microbial metabolism of dichloroacetate

Project description:Anaerobic degradation of dichloroacetate (DCA) in the environment is not well understood. Recently, a microbial mixed culture (RM) was derived from pristine freshwater sediment enriched with dichloromethane (CH2Cl2, DCM) as the sole energy source under anoxic conditions. RM comprises of Candidatus Dichloromethanomonas elyunquensis strain RM which cannot only utilize DCM as an energy source, but also DCA, with the transient formation of formate, H2, and carbon monoxide (CO). To understand the metabolic capabilities of Ca. Dichloromethanomonas elyunquensi, a comparative global proteomic analysis between DCA-grown (MS datasets 1,2,3 - T8,T15) and DCM-grown (MS datasets 4,5,6 - T12,T15) cells was performed. Differential protein expression analysis identified enzymes catalyzing the conversion of DCA to acetyl-coenzyme A (CoA) via glyoxylate as well as enzymes of the Wood-Ljungdahl pathway in DCA fermentation. Combined with physiological, biochemical, and genomics information, these results demonstrate the involvement of a haloacid dehalogenase and the Wood-Ljungdahl pathway in the anaerobic metabolism of DCA, which has broader implications in our understanding of DCA turnover in natural and engineered environments.

2020-11-25 | MSV000086520 | MassIVE

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