Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Stress response of Saccharomyces cerevisiae to exposure to natural product Azinomycin B.

ABSTRACT: Stress responses to DNA damaging agents show characteristic responses. The effect of Azinomycin B upon cultured yeast cells in growth conditions appears to be significant. We used microarrays to detail the global programme of gene expression induced in the stress response to Azinomycin B. Keywords: stress response

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE4311 | GEO | 2006/05/30

SECONDARY ACCESSION(S): PRJNA94849

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Transcription profiling of effect of Azinomycin B on cultured Saccharomyces cerevisiae cells

Project description:Stress responses to DNA damaging agents show characteristic responses. The effect of Azinomycin B upon cultured yeast cells in growth conditions appears to be significant. We used microarrays to detail the global programme of gene expression induced in the stress response to Azinomycin B. Experiment Overall Design: Saccharomyces cerevisiae cells were exposed to selected doses of Azinomycin B for a period of 6 hours incubation for RNA extraction and hybridization on Affymetrix microarrays. We sought to compare stress response states of 100 and 10 ug/ml to control growth.

2007-09-21 | E-GEOD-4311 | biostudies-arrayexpress

Stress response of Saccharomyces cerevisiae to exposure to natural product pyocyanin.

2007-01-22 | GSE6185 | GEO

Transcription profiling of S. cerevisiae stress response to exposure of the natural product pyocyanin.

Project description:Pyocyanin has been shown to engage in redox transfer of electrons from NADPH to oxygen to generate superoxide radicals. Transcriptional response to oxygen stress has been characterized in yeast and should be observable upon exposure to pyocyanin if this is the true mode of action. We used microarrays to detail the global programme of gene expression induced in the stress response to pyocyanin. Experiment Overall Design: Saccharomyces cerevisiae cells were exposed to 25 and 250 ug/ml of pyocyanin for a period of 6 hours incubation for RNA extraction and hybridization on Affymetrix microarrays. Results were compared to transcription levels in untreated cells under the same incubations conditions. The data indicated that transcriptional changes were consistent with an oxidative stress response, as observed by other researchers investigating the mode of action of pyocyanin toxicity.

2008-06-14 | E-GEOD-6185 | biostudies-arrayexpress

Saccharomyces cerevisiae

Project description:Yeast response to the DNA damaging beta-lapachone

| PRJNA117087 | ENA

Saccharomyces cerevisiae

Project description:Yeast Stress Response

| PRJNA84543 | ENA

Expression data for UV time course in Saccharomyces cerevisiae wild-type and mutant strains

Project description:Treatment of cells with DNA damaging agents leads to large-scale gene expression changes. Proper transcriptional regulation is important for cells to arrest, repair damage and adjust cellular processes such as metabolism in order to survive the damaging assault. Damage-induced transcription is a highly regulated response. This study establishes a novel role for two factors, Snf1 and Rad23, in regulation of the UV-induced transcriptional response.

2009-08-18 | GSE16799 | GEO

Leveraging natural variation in the Saccharomyces cerevisiae stress response to understand lignocellulosic hydrolysate toxicity

Project description:Here, we explored natural variation in stress tolerance and in transcriptomic responses to synthetic hydrolysate, mimicking chemically pretreated plant material, to dissect the physiological effects hydrolysate components. Using six different Saccharomyces cerevisiae strains that together maximized phenotypic and genetic diversity, we explored transcriptomic differences between resistant and sensitive strains. We identified both common and strain-specific responses. Comparing responses of resistant and sensitive strains provided insights about primary cellular targets of hydrolysate toxins, implicating cell wall structure, protein and DNA stability, energy stores and redox balance. Importantly, we uncovered lower expression of thiamine genes while in the presence of toxins, which we argue are most likely an indirect effect that increases sensitivity. We also demonstrate synergistic interactions between the nutrient composition, osmolarity, pH, and classes of hydrolysate toxins. Together, this work provides a platform for further dissecting hydrolysate toxins and strain responses.

2016-04-01 | GSE77505 | GEO

Saccharomyces cerevisiae

Project description:Yeast response to acid stress

| PRJNA141227 | ENA