Genomics

Dataset Information

0

Myc correlates globally with transcription complexes rather than specific DNA elements


ABSTRACT: The Myc-Max heterodimer has been thought of as a sequence specific DNA binding protein that regulates transcription of a large number of genes. We demonstrate here that the positions of the human genome occupied by Myc-Max correlate with the RNA polymerase II (Pol II) transcription complex rather than to the canonical DNA binding sequence element CACGTG. The heterodimer is positioned slightly upstream of essentially all promoter proximal paused polymerases and is found throughout the transcribed regions of genes. Using a multi-genome analysis of promoter regions we show that the heterodimers are oriented with respect to the direction of transcription with Myc downstream of Max. In strong support of a model in which the Myc is recruited by transcription complexes rather than specific DNA sequences, we found that the difference in affinities of Myc-Max heterodimers for CACGTG versus non-specific DNA is not great enough to drive the pattern of genome occupancy exhibited.

ORGANISM(S): Homo sapiens

PROVIDER: GSE43227 | GEO | 2013/01/02

SECONDARY ACCESSION(S): PRJNA185044

REPOSITORIES: GEO

Similar Datasets

2013-01-02 | E-GEOD-43227 | biostudies-arrayexpress
2014-09-22 | GSE58570 | GEO
2014-09-22 | E-GEOD-58570 | biostudies-arrayexpress
2018-08-20 | GSE115915 | GEO
2005-04-21 | GSE2542 | GEO
2010-06-10 | E-GEOD-2542 | biostudies-arrayexpress
2019-03-14 | GSE125863 | GEO
2019-03-14 | GSE107222 | GEO
2023-04-06 | GSE198873 | GEO
2016-10-18 | GSE77328 | GEO