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Genome wide-DNA methylation analysis of articular chondrocytes reveals a cluster of osteoarthritic patients

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Homo sapiens

PROVIDER: GSE43270 | GEO | 2013/04/01

SECONDARY ACCESSION(S): PRJNA185266

REPOSITORIES: GEO

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Genome wide-DNA methylation analysis of articular chondrocytes reveals a cluster of osteoarthritic patients

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

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Project description:The aim of this study is to identify, for the first time, the genome-wide DNA methylation profiles of human articular chondrocytes from OA and healtly cartilage samples. Genome wide DNA methylation profiling of normal and osteoarthritic samples. The Illumina Infinium 27k Human DNA methylation Beadchip v1.2 was used to obtain DNA methylation profiles across approximately 27,000 CpGs in cartilage knee samples. Samples included 18 healthy controls and 23 OA patients.

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Project description:The aim of this study is to identify, for the first time, the genome-wide DNA methylation profiles of human articular chondrocytes from OA and healtly cartilage samples. A subsequent validation of methylation profiles were performed in an idependent cohort of OA samples with Affymetrix Hugene 1.1 st array This represents the gene expression component of the study only

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Genome-wide mapping of DNA hydroxymethylation in osteoarthritic chondrocytes.

Project description: Not available

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Genome-wide mapping of DNA hydroxymethylation in osteoarthritic chondrocytes [methylation]

Project description:Examination of the genome-wide distribution of 5hmC in osteoarthritic chondrocytes compared to normal chondrocytes in order to elucidate the effect on OA-specific gene expression. 5hmC-sequencing was performed and data was compared with microarray gene expression data to identify genes with differential expression between normal and OA chondrocytes that are potentially under epigenetic regulation.

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