Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

IM005 - Mouse infection with PB2-627E: A/Vietnam/1203-CIP048_RG2/2004 (H5N1)

ABSTRACT: To determine the host response (C57BL/6 mouse model) to the PB2-627E: A/Vietnam/1203-CIP048_RG2/2004 (H5N1) virus at 10^4 PFU.

ORGANISM(S): Mus musculus

PROVIDER: GSE43301 | GEO | 2013/01/05

SECONDARY ACCESSION(S): PRJNA185329

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

IM005 - Mouse infection with PB2-627E: A/Vietnam/1203-CIP048_RG2/2004 (H5N1)

Project description:To determine the host response (C57BL/6 mouse model) to the PB2-627E: A/Vietnam/1203-CIP048_RG2/2004 (H5N1) virus at 10^4 PFU. Groups of 20-week-old female C57BL/6 mice were infected with the PB2-627E: A/Vietnam/1203-CIP048_RG2/2004 (H5N1) virus at 10^4 PFU or time-matched mock infected. Time points were 1, 2, 4 and 7 d.p.i. There were 5 mice/time point for infections and 3 mice/time point for time-matched mocks. Lung samples were collected for virus load, transcriptional analysis and proteomics analysis. Weight loss and animal survival were also monitored.

2013-01-05 | E-GEOD-43301 | biostudies-arrayexpress

ICL0011 - Host response in human Calu-3 cells to infection with A/Vietnam/1203-CIP048_RG3/2004 (H5N1) (PB1-F2 deletion), A/Vietnam/1203-CIP048_RG3/2004 (H5N1) (PB2-627E mutant) or WT: A/Vietnam/1203/2004 (H5N1)

Project description:Differential expression was determined in Calu-3 cells between mock infected and infection with one of 3 Influenza viruses (wild-type VN1203, VN1203 mutant PB1-F2del, VN1203 mutant PB2-627E) at different times post infection. Purpose: To obtain samples for transcriptional analysis in triplicate using the VN1203 pathogenicity mutants PB1-F2del and PB2-627E. Overview of Experiment: . Time Points: 0, 3, 7, 12, 18 and 24 hrs post infection. . There are two time points for wild type VN1203. . Done in triplicate. . Triplicates are defined as 3 different wells, plated at the same time using the same cell stock for all replicates. . Time-matched mocks were done in triplicate from the same cell stock as the rest of the samples. . Culture medium (the same as what the virus stock is in) was used for the mock infections. Calu-3 cells were infected with A/Vietnam/1203-CIP048_RG3/2004 (H5N1) (PB1-F2 deletion), A/Vietnam/1203-CIP048_RG3/2004 (H5N1) (PB2-627E mutant) or mock infected and samples were collected at 0, 3, 7, 12, 18 and 24 hpi. Calu-3 cells were infected with WT: A/Vietnam/1203/2004 (H5N1) and samples were collected at 7 and 24 hpi. There are 3 mock and 3 infected replicates for each time point. Expression profiles were determined.

2013-01-05 | E-GEOD-43203 | biostudies-arrayexpress

IM006B - Mouse infection with A/Vietnam/1203-CIP048_RG3/2004 (H5N1)

2013-02-21 | GSE44441 | GEO

IM007 - Mouse infection with A/Vietnam/1203-CIP048_RG4/2004 (H5N1)

2013-02-21 | GSE44445 | GEO

IM007 - Mouse infection with A/Vietnam/1203-CIP048_RG4/2004 (H5N1)

Project description:A/Vietnam/1203-CIP048_RG4/2004 (H5N1) is an NS1trunc124 mutation in the wild type A/Vietman/1203/2004 (H5N1). The goal of this study was to determine the host response (C57BL/6 mouse model) to the NS1 truncation at both 10^3 PFU and 10^4 PFU. Groups of 20-week-old C57BL/6 mice were infected with the A/Vietnam/1203-CIP048_RG4/2004 (H5N1). This is an NS1trunc124 mutation in the wild type A/Vietnam/1203/2004 (H5N1) virus. Infections were done at 10^3 PFU or 10^4 PFU or time-matched mock infected. Time points were 1, 2, 4 and 7 d.p.i. There were 3-5 animals/dose/time point. Lung samples were collected for virus load, transcriptional and proteomics analysis. Weight loss and animal survival were also monitored.

2013-02-21 | E-GEOD-44445 | biostudies-arrayexpress

IM006B - Mouse infection with A/Vietnam/1203-CIP048_RG3/2004 (H5N1)

Project description:A/Vietnam/1203-CIP048_RG3/2004 (H5N1) is a PB1-F2 deletion in wild type A/Vietnam/1203/2004 (H5N1). The goal of this study was to determine the host response (C57BL/6 mouse model) to the PB1-F2 mutation at a 10^4 PFU dose. Groups of 20-week-old female C57BL/6 mice were infected with A/Vietnam/1203-CIP048_RG3/2004 (H5N1). This is a PB1-F2 deletion in wild type A/Vietnam/1203/2004 (H5N1). Infections were with 10^4 PFU or time-matched mock infections. Time points were 1, 2, 4 and 7 d.p.i. There were 1-5 animals/dose/time point. Lung samples were collected for virus load, transcriptional analysis and proteomic analysis. Weight loss and animal survival were also monitored.

2013-02-21 | E-GEOD-44441 | biostudies-arrayexpress

ICL0012 - Host response in human Calu-3 cells to infection with NS1trunc124: A/Vietnam/1203-CIP048_RG4/2004 (H5N1) or WT: A/Vietnam/1203/2004 (H5N1)

Project description:Differential expression was determined in Calu-3 cells between mock infected and infection with NS1trunc124: A/Vietnam/1203-CIP048_RG4/2004 (H5N1) or WT: A/Vietnam/1203/2004 (H5N1) at different times post infection.

2013-01-05 | GSE43204 | GEO

IM006A - Mouse infection with PB1-F2del: A/Vietnam/1203-CIP048_RG3/2004 (H5N1) at 10^3 PFU

Project description:To determine the host response (C57BL/6 mouse model) to the PB1-F2del: A/Vietnam/1203-CIP048_RG3/2004 (H5N1) virus at 10^3 PFU.

2013-01-19 | GSE43302 | GEO

IM013 - Mouse infection with A/Vietnam/1203/2004 (H5N1)

Project description:To determine the host response (C57BL/6 mouse model) to A/Vietnam/1203/2004 (H5N1) virus at 10^3 and 10^4PFU. Groups of 22-week-old C57BL/6 mice were infected with A/Vietnam/1203/2004 (H5N1) virus at 10^3 or 10^4 PFU or time-matched mock infected. Time points were 1, 2, 4 and 7 d.p.i. There were 3-4 animals/dose/time point. Lung samples were collected for virus load, transcriptional and proteomic analysis. Weight loss and animal survival were also monitored.

2014-04-02 | E-GEOD-56433 | biostudies-arrayexpress

Mouse lung tissue transcriptome response to H1N1, H5N1, and mutants [miRNA]

Project description:The purpose is to obtain samples for mRNA, miRNA, proteomics, lipidomics, metabolomics, and histopathology analysis in mouse lung infected with WT A/Vietnam/1203/2004 (H5N1; 'VN1203'), VN12013-PB2-627E, VN1203-NS1trunc124, and WT A/California/04/2009 (H1N1; 'CA04'). Groups of 22-week-old C57BL/6 mice were infected with WT A/Vietnam/1203/2004 (H5N1; 'VN1203'), VN12013-PB2-627E, VN1203-NS1trunc124, and WT A/California/04/2009 (H1N1; 'CA04').Infections were done at 176 PFU/mouse for VN1203, 70 PFU/mouse for VN1203-PB2-627E, 1100 PFU/mouse for VN1203-NS1trunc124, and 10^4 PFU/mouse for CA04. Time points were 1, 2, 4, and 7 d.p.i. There were 8 mice for all infection groups and 5 mice for all mock groups. Lung samples were collected for virus load, transcriptional and proteomics analysis. Weight loss and animal survival were also monitored.

2015-06-18 | E-GEOD-69944 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data