Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Mouse fetal liver cells: treated with nanosilver particles (nAg) vs control.

ABSTRACT: To investigate the differently expressed genes upon the nanosilver particle (nAg) exposure.

ORGANISM(S): Mus musculus

PROVIDER: GSE43331 | GEO | 2016/01/31

SECONDARY ACCESSION(S): PRJNA185439

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Impact of exposure to environmentally-relevant concentrations of ionic silver and nanosilver on thyroid hormone-dependent postembryonic development of the North American bullfrog (Rana catesbeiana)

Project description:Nanoparticles (<100 nm) are engineered to have unique physico-chemical properties compared to their larger counterparts. Nanosilver (nAg) is the most prevalent nanoparticle in consumer products due to its strong antimicrobial action. While nAg toxicity at high concentrations has been well described, the potential for sublethal effects at or below regulatory guidelines is relatively unknown. Amphibian metamorphosis is mediated by thyroid hormone (TH) and can be precociously induced by the addition of exogenous hormone. Low concentrations of nAg have been shown to disrupt TH-dependent responses in cultured premetamorphic Rana catesbeiana tadpole tail fin. The present study examined the effects of exposure to an environmentally relevant concentration (6 µg/L) of either nAg or ionic silver (iAg) on premetamorphic tadpoles in the absence and presence of exogenous TH. Disruption of thyroid hormone-mediated signalling in brain and liver transcriptomes was evaluated using the MAGEX cDNA microarray.

2017-03-31 | GSE56523 | GEO

The physicochemistry of capped nanosilver predicts it biological activity in brain endothelial (RBEC4) cells

Project description:This study uses the RBEC4 cell model to examine if nanosilver of different sizes (10nm and 75nm) and cappings (citrate and polyvinylpyrrolidone(PVP)) affect its cellular permeability and genomic response. RBEC4 cells (95% confluent) were screeened for changes in gene expression after 18 hour exposure to 1.0 ppm of PVP or citrate coated nanosilver particles at 10 or 75 nanometer sizes. Analysis indicated that the cellular transcription profile alter by nanosilver 10nm+PVP was distinct from it 75nm counterpart and both sizes of citrate-coated particles.

2013-05-31 | E-GEOD-47543 | biostudies-arrayexpress

The physicochemistry of capped nanosilver predicts it biological activity in brain endothelial (RBEC4) cells

2013-05-31 | GSE47543 | GEO

Chronic sublethal exposure to silver nanoparticles disrupts thyroid hormone signaling during Xenopus laevis metamorphosis

Project description:Nanoparticles (NPs) are engineered in the nanoscale (<100nm) to have unique physico-chemical properties from their bulk counterparts. Nanosilver (nAg) is the most prevalent nanoparticle in consumer products due to its strong antimicrobial action. While nAg toxicity at high concentrations has been well described, the sublethal effects at or below regulatory guidelines are relatively unknown. Amphibian metamorphosis is mediated by thyroid hormone (TH), and initial studies indicate that low concentrations of nAg disrupt TH-dependent responses in precociously induced premetamorphic bullfrog (Rana catesbeiana) tadpoles. The present study examined the effects of environmentally-relevant nAg concentrations (LoAg, 0.018 µg/L; MedAg, 0.18 µg/L; HiAg, 1.8 µg/L) on naturally metamorphosing Xenopus laevis tadpoles in two 28-day chronic exposures beginning with pre- and prometamorphic stages, respectively. nAg was found to significantly bioaccumulate in tadpoles after 28 days. While nAg didn’t alter metamorphic timing, it increased hindlimb length during early premetamorphosis and in post-metamorphic juvenile tadpoles. Using MAGEX microarray and QPCR transcript analyses, 7 markers of nAg exposure were discovered and validated, 5 of which showed nAg-induced disruption of their TH-response. The increased mRNA abundance of two peroxidase genes suggests that nAg could generate reactive oxygen species (ROS) even at low, environmental concentrations. Furthermore, differential responsiveness to nAg was observed between developmental stages. Therefore, low concentrations of nAg had endocrine disruptive effects at both the physiological and molecular level, indicating that regulatory guidelines for silver may need revision.

2016-12-23 | GSE56012 | GEO

Maize microbiome under nanosilver exposure

Project description:Metatranscriptome analysis of maize rhizosphere microbiome under nanosilver exposure

| PRJEB22046 | ENA

Proteomic and morphological effect of nanosilver on soybean under flooding stress

Project description:Flooding stress has a negative impact on the soybean cultivation in early growth stages. In order to understand the effect of nanosilver on the soybean growth under flooding stress, quantitative proteomics technique was used.

2016-12-14 | PXD001579 | Pride

Transcription profiling of mouse Apoe knockouts treated with SRM2975 and C57Bl/6 mice treated with exhaust particles reveals Serum amyloid and serum amyloid P are not induced in response to diesel exhaust particles or carbon black

Project description:W evaluated an experimental particle exposure model, including markers of the acute phase system assessed as the hepatic mRNA expression of Sap, the murine homologue of CRP, and Saa1 and Saa3. Experiment Overall Design: 12-13 weeks old Apoe knockout mice (C57BL/6-Apoe tm1) mice were injected intraperitoneally with varying doses of SRM 2975. In a parallel experiment C57BL mice were exposed by inhalation for 90 minutes to four consecutive doses of 20mg/m3 of filtered air, NIST 2975 or Ptintex 90. Livers and aortic tissue were collected.

2009-05-02 | E-GEOD-11346 | biostudies-arrayexpress

Pathways identified by toxicogenomics analysis reveal the size and dose independency of silica particles-induced toxicity in mice.

Project description:Understanding the interactions of nanostructures with biological systems is essential to nanotoxicological research. Using a microarray-based toxicogenomics approach at early stage, this study investigated the relationship between particle size and toxicity of silica particles (SP) with diameters of 30, 70, and 300 nm (SP30, SP70, and SP300) as well as the mechanism of injury in mice. Two experiments with SiO2 particles of different sizes were considered in mice. One was aimed to investigate the dose-response relationship of SP70 toxicity at a dose of 10, 20, or 40 mg/kg (experiment 1), and the other set to study the size-response relationship of SP-induced toxicity using SP30, SP70, or SP300 (experiment 2). In experiment 2, two dosages each of SP30, SP70, and SP300 were performed. One was 10 mg/kg at three particle sizes, and the other was toxic doses of the three particle sizes, i.e., 10 mg/kg for SP30, 40 mg/kg for SP70, and 200 mg/kg for SP300. The toxic doses of the three particle sizes of SP were decided on the basis of the results of histopathological examinations and serum biochemical analysis in our previous study.n = 5

2011-07-31 | E-GEOD-30861 | biostudies-arrayexpress

Bifidobacterium infantis utilizes N-acetylglucosamine nitrogen

Project description:15N-labeled N-acetylglucosamine (NAG) fed to B. infantis cells are incorporated into its proteome. This indicates that NAG, an amino sugar residue of human milk oligosaccharides (HMO) and other biopolymers, is used as a nitrogen source. Transcriptomics while subsisting on NAG nitrogen are consistent with the proteomics results. This further indicates that B. infantis utilizes NAG nitrogen in and shunts it towards a fundamental cellular processes.

2023-05-18 | PXD035809 | Pride

Human breast cancer cells: control vs treated with breast cancer patients' sera

Project description:To investigate the significant differently expressed genes upon the stimulation of breast cancer patients' sera.

2012-08-15 | GSE40108 | GEO