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Comparative expression profiling of miRNA during anther development in genetic male sterile and wild type cotton [small RNA]

ABSTRACT: Genetic male sterility (GMS) in cotton (Gossypium hirsutum) plays an important role in the utilization of hybrid vigor. However, the molecular mechanism of the GMS is still unclear. While numerous studies have demonstrated that microRNAs (miRNA) regulate flower and anther development, whether different small RNA regulations exist in GMS and its wild type is unclear. To investigate the global expression and complexity of small RNAs during cotton anther development, three small RNA libraries were constructed from the anthers of three development stages each from fertile wild type (WT) and its GMS mutant cotton.

ORGANISM(S): Gossypium hirsutum

PROVIDER: GSE43531 | GEO | 2013/07/28

SECONDARY ACCESSION(S): PRJNA186926

REPOSITORIES: GEO

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Comparative expression profiling of miRNA during anther development in genetic male sterile and wild type cotton [small RNA]

Project description:Genetic male sterility (GMS) in cotton (Gossypium hirsutum) plays an important role in the utilization of hybrid vigor. However, the molecular mechanism of the GMS is still unclear. While numerous studies have demonstrated that microRNAs (miRNA) regulate flower and anther development, whether different small RNA regulations exist in GMS and its wild type is unclear. To investigate the global expression and complexity of small RNAs during cotton anther development, three small RNA libraries were constructed from the anthers of three development stages each from fertile wild type (WT) and its GMS mutant cotton. Examination of different miRNA profiles in 2 lines.

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Identification of anther expressing genes in cotton (Gossypium hirsutum)

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Genome-wide profiling of miRNAs and other small non-coding RNAs in the Verticillium dahliae inoculated cotton roots

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