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Directional RNA sequencing of mRNA from Sinorhizobium meliloti cells grown with limiting phosphate and with excess phosphate.

ABSTRACT: Analysis of the RNA-seq reads confirmed that transcription of many of the previously identified Pho-regulon genes of Sinorhizobium meliloti was regulated by the availability of inorganic phosphate in the media. The transcriptional start sites of many of the Pho-regulon genes as determined by RNA-seq reads were found to correspond to those determined from primer extension analysis.

ORGANISM(S): Sinorhizobium meliloti

PROVIDER: GSE43558 | GEO | 2013/06/01

SECONDARY ACCESSION(S): PRJNA186705

REPOSITORIES: GEO

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Directional RNA sequencing of mRNA from Sinorhizobium meliloti cells grown with limiting phosphate and with excess phosphate.

Project description:Analysis of the RNA-seq reads confirmed that transcription of many of the previously identified Pho-regulon genes of Sinorhizobium meliloti was regulated by the availability of inorganic phosphate in the media. The transcriptional start sites of many of the Pho-regulon genes as determined by RNA-seq reads were found to correspond to those determined from primer extension analysis. mRNA from S. meliloti RmP110 grown in MOPS + 2 mM Pi and MOPS + 20 M-BM-5M Pi M-bM-^@M-^S directional RNA-seq via Illumina sequencing

2013-06-01 | E-GEOD-43558 | biostudies-arrayexpress

A Sinorhizobium meliloti IAA-overproducing strain improves phosphate solubilization and Medicago plant yield

Project description:This SuperSeries is composed of the following subset Series: GSE21735: Sinorhizobium meliloti cells: untreated 1021 strain vs. 1021 treated with 2,4-dichlorophenoxyacetic acid (2,4-D) GSE21737: Sinorhizobium meliloti cells: untreated 1021 strain vs. 1021 treated with indole-3-acetic acid (IAA) GSE21740: Sinorhizobium meliloti cells: untreated 1021 strain vs. 1021 treated with indole-3-carboxylic acid (ICA) GSE21742: Sinorhizobium meliloti cells: untreated 1021 strain vs. 1021 treated with indole (IND) GSE21743: Sinorhizobium meliloti cells: untreated 1021 strain vs. 1021 treated with tryptophan (Trp) GSE21744: Sinorhizobium meliloti cells: untreated 1021 wild type strain vs. RD64 strain Refer to individual Series

2010-05-20 | E-GEOD-21745 | biostudies-arrayexpress

Transcription profiling by array of Sinorhizobium meliloti genes regulated by RpoE4 in the presence of thiosulfate

Project description:The Sinorhizobium meliloti rpoE4 regulon was determined by comparing the wildtype and rpoE4 mutant transcriptomes in the presence of 20mM sodium thiosulfate, an RpoE4 activating condition.

2012-11-01 | E-MEXP-3475 | biostudies-arrayexpress

Sinorhizobium meliloti as a model system to study Ca. Liberibacter asiaticus regulators. [CtrA]

Project description:We characterized transcriptomes of a Sinorhizobium meliloti wild type strain (CL150) expressing either Ca. Liberibacter asiaticus ctrA or Sinorhizobium meliloti ctrA

2019-08-01 | GSE124977 | GEO

Sinorhizobium meliloti as a model system to study Ca. Liberibacter asiaticus regulators. [RpoH]

Project description:We characterized transcriptomes of a Sinorhizobium meliloti rpoH1rpoH2 deletion mutant (RFF231; Lang et al. 2018, mSphere 3:e00454-18) expressing either Ca. Liberibacter asiaticus rpoH or Sinorhizobium meliloti rpoH1

2019-08-01 | GSE124982 | GEO

Novel genes and regulators for cell surface of Sinorhizobium meliloti.

Project description:We characterized the transcriptome of a Sinorhizobium meliloti emmA insertion mutant strain.

2017-11-30 | GSE100707 | GEO

The small proteome of the nitrogen-fixing plant symbiont Sinorhizobium meliloti

Project description:The Alphaproteobacterium Sinorhizobium meliloti lives in soil and is capable of fixing molecular nitrogen in symbiosis with legume plants. In this work, the small proteome of S. meliloti strain 2011 was studied to uncover translation of both annotated and novel small open reading frame (sORF)-encoded proteins (SEPs).

2023-03-13 | PXD034931 | Pride

Expression analysis of Sinorhizobium meliloti 1021rpoH1 rpoH2 mutant during heat shock

Project description:Investigation of whole genome gene expression level changes in a Sinorhizobium meliloti 1021 rpoH1 rpoH2 double mutant, compared to the wild-type strain. The mutations engineered into this strain render it deficient in symbiotic nitrogen fixation. The mutants analyzed in this study are further described in Mitsui, H, T. Sato, Y. Sato, and K. Minamisawa. 2004. Sinorhizobium meliloti RpoH1 is required for effective nitrogen-fixing symbiosis with alfalfa. Mol Gen Genomics 271:416-425.

2014-11-01 | GSE62900 | GEO

Overexpression of Sinorhizobium meliloti ECF sigma factors.

Project description:We characterized transcriptomes for strains overexpressing each of the Sinorhizobium meliloti ECF sigma factors the via a plasmid-borne, melibiose-inducible promoter plasmid (PmelA; pCAP11: Pinedo et al. 2008 J Bacteriol 190:2947-2956) compared to control strains carrying the empty vector.

2018-09-05 | GSE116665 | GEO

Identification of RpoE2 target genes in Sinorhizobium meliloti

Project description:Sinorhizobium meliloti lives as a soil saprophyte, and engages in a nitrogen fixing symbiosis with plant roots. To succeed in such diverse environments, the bacteria must continually adjust gene expression. Transcriptional plasticity in eubacteria is often mediated by alternative sigma factors interacting with core RNA polymerase. The S. meliloti genome encodes 14 of these alternative sigmas, including 11 extracytoplasmic function (ECF) sigmas. We used custom Affymetrix Symbiosis Chips to characterize the global transcriptional response of S. meliloti overexpressing the ECF sigma factor, RpoE2. Our work identifies over 200 genes whose expression is dependent on RpoE2.

2013-07-22 | GSE40391 | GEO

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