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The interactome of POU5F1 upstream enhancer in mouse embryonic stem cells revealed by 4C-Seq

ABSTRACT: We report the application of 4C-Seq technique for exploring POU5F1 enhancer interactome in mouse embryonic stem cells. A statistical model was built to identify enriched interacting regions from raw 4C data. The biological replicate data were compared to identify reproducible interacting regions. The interacting sites in the reproducible regions are enriched with active histone marks as well as transcription factors Oct4, Klf4, Esrrb, Tcfcp2i1 and Zfx that are critical for reprogramming and pluripotency.

ORGANISM(S): Mus musculus

PROVIDER: GSE43776 | GEO | 2013/04/28

SECONDARY ACCESSION(S): PRJNA187390

REPOSITORIES: GEO

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The interactome of POU5F1 upstream enhancer in mouse embryonic stem cells revealed by 4C-Seq

Project description:We report the application of 4C-Seq technique for exploring POU5F1 enhancer interactome in mouse embryonic stem cells. A statistical model was built to identify enriched interacting regions from raw 4C data. The biological replicate data were compared to identify reproducible interacting regions. The interacting sites in the reproducible regions are enriched with active histone marks as well as transcription factors Oct4, Klf4, Esrrb, Tcfcp2i1 and Zfx that are critical for reprogramming and pluripotency. Generation of Illumina HiSeq2000 sequencing data using 4C-Seq protocol

2013-04-28 | E-GEOD-43776 | biostudies-arrayexpress

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The interactome of Pou5f1 enhancer in mouse embryonic stem cells

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Zygotic gene activatory network in zebrafish is globally controlled by Pou5f1 SoxB1 enhancers

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Zygotic gene activatory network in zebrafish is globally controlled by Pou5f1 SoxB1 enhancers

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Project description:Our objective is to clarify the function of EWS-POU5F1 chimera. Specifially, GBS6 cells were established from an undifferentiated bone sarcoma carrying translocation t(6;22)(p21;q12). The translocation resulted in a gene fusion between EWS and POU5F1. Gene expression analysis of t(6;22) undifferentiated sarcoma cell line GBS6 transfected with POU5F1 specific siRNA to investigate the function of EWS-POU5F1.

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Differential gene expression in GBS6 cells after EWS-POU5F1 knockdown

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Experiment 2: Pou5f1 and Sox2 overexpression experiments with or without protein synthesis inhibition

Project description:Pou5f1 and Sox2 overexpression experiments with and without protein synthesis inhibitor were performed to investigate direct transcriptional targets of Pou5f1 and Sox2

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